US4511503A
Expired / Lapsed
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Purification and activity assurance of precipitated heterologous proteins
Assignee: Genentech Inc
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Filing: 1984-06-01
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Granted: 1985-04-16
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Expires: 2004-06-01
Abstract
Abstract A process for dissolving refractile proteins from their insoluble form by using a strongly denaturing solution.
Inventors
- 👤 Kenneth C. Olson
- 👤 Rong-Chang Pai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 20:26
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6478; C12N9/6481; C07; C07K; C07K1/00; C07K1/107; C07K1/113; C07K1/1133; C12N9/6424; C12N9/6456; C12N9/6462; C12Y; C12Y304/00; C12Y304/21; C12Y304/21073; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S530/00; Y10S530/806; Y10S530/808; Y10S530/82; Y10S530/825; Y10S530/826
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
68 words
A method of solubilizing heterologous protein, produced in an insoluble "refractile form in a recombinant host cell culture, which method comprises: isolating and insoluble "refractile" heterologous protein from said host cell culture, and treating said protein with a strongly denaturing solution selected from the group consisting of a guanidine salt or a salt of thiocyanate having a concentration between about 4M and 9M to solubilize said refractile protein.
Claim 2
Independent Claim
68 words
A method of solubilizing heterologous protein, produced in an insoluble "refractile form in a recombinant host cell culture, which method comprises: isolating and insoluble "refractile" heterologous protein from said host cell culture, and treating said protein with a strongly denaturing solution selected from the group consisting of a guanidine salt or a salt of thiocyanate having a concentration between about 4M and 9M to solubilize said refractile protein.
Claim 3
Dependent Claim
14 words
The process of claim 1 wherein the denaturant is guanidine hydrochloride or sodium thiocyanate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-04-15 | 1988-10-15 | 1989-04-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-04-15 | 1992-10-15 | 1993-04-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-04-15 | 1996-10-15 | 1997-04-16 | $7700.00 | Window Closed / Lapsed |