EP0057350A2
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EP
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Recombinant DNA means and method
Assignee: Oscient Pharmaceuticals Corp
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Filing: 1982-01-08
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Granted: —
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Expires: 2002-01-08
Abstract
Abstract Living cells containing genetic material derived from recombinant DNA material and capable of expressing rennin, pre-prorennin and prorennin. The rennin, pre- prorennin and prorennin are derived from cells which are themselves or have had parents thereof treated by recombinant DNA methods to allow production of the desired anzymatic proteins during growth in culture.
Inventors
- 👤 Bernadette Lavetsky Alford
- 👤 Donald Taylor Moir
- 👤 Gerald Francis Vovis
- 👤 Jen-I Mao
- 👤 Alison Taunton-Rigby
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 15:59
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6478; C12N9/6481; C07; C07K; C07K14/00; C07K14/37; C07K14/39; C12N1/00; C12N1/14; C12N1/16; C12N1/18; C12N1/185; C12N1/20; C12N1/205; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C07K2319/00; C07K2319/01; C07K2319/02; C12R; C12R2001/00; C12R2001/01; C12R2001/185; C12R2001/19; C12R2001/645; C12R2001/85; C12R2001/865
Claims Analysis
Total Claims: 66
Claim 1
Independent Claim
16 words
A living cell containing genetic material derived from recombinant DNA material and capable of expressing rennin.
Claim 2
Dependent Claim
16 words
A living cell containing genetic material derived from recombinant DNA material and capable of expressing rennin.
Claim 3
Dependent Claim
16 words
A living cell containing genetic material derived from recombinant DNA material and capable of expressing pre-prorennin.
Claim 4
Dependent Claim
16 words
A living cell containing genetic material derived from recombinant DNA material and capable of expressing pre-prorennin.
Claim 5
Dependent Claim
16 words
A living cell containing genetic material derived from recombinant DNA material and capable of expressing prorennin.
Claim 6
Dependent Claim
16 words
A living cell containing genetic material derived from recombinant DNA material and capable of expressing prorennin.
Claim 7
Dependent Claim
19 words
Rennin derived from a living cell containing genetic material obtained from recombinant DNA material and capable of expressing rennin.
Claim 8
Dependent Claim
19 words
Rennin derived from a living cell containing genetic material obtained from recombinant DNA material and capable of expressing rennin.
Claim 9
Dependent Claim
19 words
Pre-prorennin derived from a living cell containing genetic material obtained from recombinant DNA material and capable of expressing pre-prorennin.
Claim 10
Dependent Claim
19 words
Pre-prorennin derived from a living cell containing genetic material obtained from recombinant DNA material and capable of expressing pre-prorennin.
Claim 11
Dependent Claim
19 words
Prorennin derived from a living cell containing genetic material obtained from recombinant DNA material and capable of expressing prorennin.
Claim 12
Dependent Claim
19 words
Prorennin derived from a living cell containing genetic material obtained from recombinant DNA material and capable of expressing prorennin.
Claim 13
Independent Claim
84 words
A method of obtaining expression of pre-prorennin in host cells, said method comprising isolating a DN A sequence that codes for pre-prorennin, attaching a transcriptional promoter and attaching ribosomal binding sites to that sequence at the 5' end and varying the distance between the beginning of the DNA that codes for pre-prorennin and the segment of DNA carrying the promoter and binding site, transforming the DNA into host cells, cloning the host cells and selecting those that have high levels of expression of pre-prorennin.
Claim 14
Independent Claim
84 words
A method of obtaining expression of pre-prorennin in host cells, said method comprising isolating a DN A sequence that codes for pre-prorennin, attaching a transcriptional promoter and attaching ribosomal binding sites to that sequence at the 5' end and varying the distance between the beginning of the DNA that codes for pre-prorennin and the segment of DNA carrying the promoter and binding site, transforming the DNA into host cells, cloning the host cells and selecting those that have high levels of expression of pre-prorennin.
Claim 15
Independent Claim
124 words
A method of obtaining expression of prorennin in host cells, said method comprising, isolating a DNA sequence that codes for pre-prorennin and having a 5'end, removing a portion from the 5' end which portion codes for the prorennin precursor polypeptide, ligating onto the remainder a synthetic piece of DNA carrying a translational initiation codon at the 3' end of said piece, attaching a transcriptional promoter and attaching a ribosomal binding site to the sequence at the 5' end and varying the distance between the beginning of the DNA that codes for prorennin and the segment of DNA carrying the promoter and binding site, transforming the DNA into host cells, cloning the host cells and selecting those that have high levels of expression of prorennin.
Claim 16
Independent Claim
124 words
A method of obtaining expression of prorennin in host cells, said method comprising, isolating a DNA sequence that codes for pre-prorennin and having a 5'end, removing a portion from the 5' end which portion codes for the prorennin precursor polypeptide, ligating onto the remainder a synthetic piece of DNA carrying a translational initiation codon at the 3' end of said piece, attaching a transcriptional promoter and attaching a ribosomal binding site to the sequence at the 5' end and varying the distance between the beginning of the DNA that codes for prorennin and the segment of DNA carrying the promoter and binding site, transforming the DNA into host cells, cloning the host cells and selecting those that have high levels of expression of prorennin.
Claim 17
Independent Claim
124 words
A method of obtaining expression of rennin in host cells, said method comprising, isolating a DNA sequence that codes for pre-prorennin and having a 5'end, removing a portion from the 5' end which portion codes for the rennin precursor polypeptide, ligating onto the remainder a synthetic piece of DNA carrying a translational initiation codon at the 3' end of said piece, attaching a transcriptional promoter and attaching a ribosanal binding site to the sequence at the 5' end and varying the distance between the beginning of the DNA that codes for prorennin and the segment bf DNA carrying the promoter and binding site, transforming the DNA into host cells, cloning the host cells and selecting those that have high levels of expression of rennin.
Claim 18
Independent Claim
124 words
A method of obtaining expression of rennin in host cells, said method comprising, isolating a DNA sequence that codes for pre-prorennin and having a 5'end, removing a portion from the 5' end which portion codes for the rennin precursor polypeptide, ligating onto the remainder a synthetic piece of DNA carrying a translational initiation codon at the 3' end of said piece, attaching a transcriptional promoter and attaching a ribosanal binding site to the sequence at the 5' end and varying the distance between the beginning of the DNA that codes for prorennin and the segment bf DNA carrying the promoter and binding site, transforming the DNA into host cells, cloning the host cells and selecting those that have high levels of expression of rennin.
Claim 19
Dependent Claim
34 words
In a method of obtaining rennin by recombinant DNA means the step of obtaining rennin genetic material from pre-prorennin genetic material after which said rennin genetic material is transformed into host cells and cloned.
Claim 20
Dependent Claim
34 words
In a method of obtaining rennin by recombinant DNA means the step of obtaining rennin genetic material from pre-prorennin genetic material after which said rennin genetic material is transformed into host cells and cloned.
Claim 21
Dependent Claim
34 words
In a method of obtaining prorennin by recombinant DNA means the step of obtaining prorennin genetic material from pre-prorennin genetic material after which said prorennin genetic material is transformed into host cells and cloned.
Claim 22
Dependent Claim
34 words
In a method of obtaining prorennin by recombinant DNA means the step of obtaining prorennin genetic material from pre-prorennin genetic material after which said prorennin genetic material is transformed into host cells and cloned.
Claim 23
Independent Claim
21 words
A method of obtaining pre-prorennin by growing cells containing genetic material obtained from recombinant DNA material and capable of expressing pre-prorennin.
Claim 24
Independent Claim
21 words
A method of obtaining pre-prorennin by growing cells containing genetic material obtained from recombinant DNA material and capable of expressing pre-prorennin.
Claim 25
Independent Claim
21 words
A method of obtaining prorennin by growing cells containing genetic material obtained from recombinant DNA material and capable of expressing prorennin.
Claim 26
Independent Claim
21 words
A method of obtaining prorennin by growing cells containing genetic material obtained from recombinant DNA material and capable of expressing prorennin.
Claim 27
Independent Claim
21 words
A method of obtaining rennin by growing cells containing genetic material obtained from recanbinant DNA material and capable of expressing rennin.
Claim 28
Independent Claim
21 words
A method of obtaining rennin by growing cells containing genetic material obtained from recanbinant DNA material and capable of expressing rennin.
Claim 29
Dependent Claim
27 words
A prorennin gene comprising a nucleotide sequence coding for the amino acid sequence of prorennin and excluding any intervening nucleotide sequences present in genomic DNA encoding prorennin.
Claim 30
Dependent Claim
27 words
A prorennin gene comprising a nucleotide sequence coding for the amino acid sequence of prorennin and excluding any intervening nucleotide sequences present in genomic DNA encoding prorennin.
Claim 31
Dependent Claim
27 words
A pre-prorennin gene comprising a nucleotide sequence coding for the amino acid sequence of pre-prorennin and excluding any intervening nucleotide sequences present in genomic DNA encoding pre-prorennin.
Claim 32
Dependent Claim
27 words
A pre-prorennin gene comprising a nucleotide sequence coding for the amino acid sequence of pre-prorennin and excluding any intervening nucleotide sequences present in genomic DNA encoding pre-prorennin.
Claim 33
Dependent Claim
27 words
A rennin gene comprising a nucleotide sequence coding for the amino acid sequence of rennin and excluding any intervening nucleotide sequences present in genomic DNA encoding rennin.
Claim 34
Dependent Claim
27 words
A rennin gene comprising a nucleotide sequence coding for the amino acid sequence of rennin and excluding any intervening nucleotide sequences present in genomic DNA encoding rennin.
Claim 35
Dependent Claim
22 words
A prorennin gene in accordance with claim 15 and having operatively attached thereto a vector which replicates in a suitable host cell.
Claim 36
Dependent Claim
22 words
A pre-prorennin gene in accordance with claim 16 and having operatively attached thereto a vector which replicates in a suitable host cell.
Claim 37
Dependent Claim
22 words
A rennin gene in accordance with claim 17 and having operatively attached thereto a vector which replicates in a suitable host cell.
Claim 38
Dependent Claim
13 words
E. coli as deposited in The American Type Culture Collection Accession number 31929.
Claim 39
Dependent Claim
13 words
E. coli as deposited in The American Type Culture Collection Accession number 31929.
Claim 40
Dependent Claim
14 words
Recombinant DNA material found in E. coli American Type Culture Collection Accession number 31929.
Claim 41
Dependent Claim
14 words
Recombinant DNA material found in E. coli American Type Culture Collection Accession number 31929.
Claim 42
Dependent Claim
9 words
Saccharomyces cerevisiae American Type Culture Collection Accession number 20623.
Claim 43
Dependent Claim
9 words
Saccharomyces cerevisiae American Type Culture Collection Accession number 20623.
Claim 44
Dependent Claim
14 words
Recombinant DNA material found in Saccharomyces cerevisiae American Type Oulture Collection Accession number 20623.
Claim 45
Dependent Claim
14 words
Recombinant DNA material found in Saccharomyces cerevisiae American Type Oulture Collection Accession number 20623.
Claim 46
Dependent Claim
16 words
Recombinant DNA material comprising a gene coding for at least one polypeptide displaying milk clotting activity.
Claim 47
Dependent Claim
16 words
Recombinant DNA material comprising a gene coding for at least one polypeptide displaying milk clotting activity.
Claim 48
Dependent Claim
20 words
A polypeptide derived from recanbinant DNA material which can be cleaved to obtain another polypeptide which displays milk clotting activity.
Claim 49
Dependent Claim
20 words
A polypeptide derived from recanbinant DNA material which can be cleaved to obtain another polypeptide which displays milk clotting activity.
Claim 50
Dependent Claim
10 words
Recombinant DNA material comprising prorennin nucleotide sequence CLaim 27 continued:
Claim 51
Dependent Claim
16 words
Recombinant DNA material comprising pre-prorennin nucleotide sequence Claim 28 continued: Claim 28 continued: CLaim 28 continued:
Claim 52
Dependent Claim
10 words
Recombinant DNA material comprising rennin nucleotide sequence Claim 29 continued:
Claim 53
Dependent Claim
25 words
Recombinant DNA material coding for a polypeptide signal sequence and having the following nucleotide and polypeptide sequence or other nucleotide sequence coding the said polypeptide
Claim 54
Dependent Claim
25 words
Recombinant DNA material coding for a polypeptide signal sequence and having the following nucleotide and polypeptide sequence or other nucleotide sequence coding the said polypeptide
Claim 55
Dependent Claim
25 words
Recombinant DNA material coding for a polypeptide zymogen sequence and having the following nucleotide and polypeptide sequence or other nucleotide sequence coding the said polypeptide
Claim 56
Dependent Claim
25 words
Recombinant DNA material coding for a polypeptide zymogen sequence and having the following nucleotide and polypeptide sequence or other nucleotide sequence coding the said polypeptide
Claim 57
Independent Claim
78 words
A method of expressing a gene coding for a polypeptide useful in clotting milk, said method comprising, selecting said gene having a 5' end, selecting a gene promoter nucleotide sequence, attaching said gene promoter nucleotide sequence to said 5' end of said gene to form a recombinant DNA material, introducing said recombinant DNA material into a cell and obtaining an enzyme produced thereby which enzyme has milk clotting activity or may be activated to have milk clotting activity.
Claim 58
Independent Claim
78 words
A method of expressing a gene coding for a polypeptide useful in clotting milk, said method comprising, selecting said gene having a 5' end, selecting a gene promoter nucleotide sequence, attaching said gene promoter nucleotide sequence to said 5' end of said gene to form a recombinant DNA material, introducing said recombinant DNA material into a cell and obtaining an enzyme produced thereby which enzyme has milk clotting activity or may be activated to have milk clotting activity.
Claim 59
Dependent Claim
42 words
A method in accordance with claim 32 and further comprising said gene having an ATG translation initiation codon at its 5' end, said promoter being a transcription promoter, and further providing a translation initiator nucleotide sequence prior to introduction into said cell.
Claim 60
Dependent Claim
16 words
A method in accordance with claim 33 wherein said translation initiator is a ribosome binding site.
Claim 61
Dependent Claim
18 words
A method of expressing a gene in accordance with claim 33 wherein said gene is a rennin gene.
Claim 62
Dependent Claim
18 words
A method of expressing a gene in accordance with claim 33 wherein said gene is a prorennin gene.
Claim 63
Dependent Claim
18 words
A method of expressing a gene in accordance with claim 33 wherein said gene is a pre-prorennin gene.
Claim 64
Dependent Claim
26 words
A method of expressing a gene in accordance with claim 33 wherein said cell is selected from the group consisting of yeast cells and bacterial cells.
Claim 65
Dependent Claim
29 words
A method of expressing a gene in accordance with claim 32 wherein said gene promoter is selected from the group consisting of yeast gene promoters and bacterial gene promoters.
Claim 66
Dependent Claim
16 words
A method in accordance with the method of claim 39 wherein said yeast is S. cerevisiae.
References Cited (Backward Citations)
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