EP0116778A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Microbially produced rennet, methods for its production and plasmids used for its production
Assignee: Genentech Inc
•
Filing: 1983-12-21
•
Granted: —
•
Expires: 2003-12-21
Abstract
Abstract The production of microbial rennet by recombinant DNA techniques is disclosed; also plasmid expression vectors for transfecting a microbial host for efficient expression of e.g. prorennin, rennin and preprorennin.
Inventors
- 👤 Michael Cheng-Yien Chen
- 👤 Kirk James Hayenga
- 👤 Virgil Bryan Lawlis
- 👤 Bradley Richard Snedecor
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 21:15
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6478; C12N9/6481; A; A23; A23C; A23C19/00; A23C19/02; A23C19/032; A23C19/0326; C07; C07K; C07K1/00; C07K1/107; C07K1/113; C07K1/1133; C12N1/00; C12N1/06; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N9/52
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
23 words
A microbial expression vector having essentially the characteristics of any one of the plasmids pRl, pRlA, pRlX, pRlAX, pR2, pR2A, pR2X and pR3.
Claim 2
Dependent Claim
23 words
A microbial expression vector having essentially the characteristics of any one of the plasmids pRl, pRlA, pRlX, pRlAX, pR2, pR2A, pR2X and pR3.
Claim 3
Dependent Claim
10 words
Microbial rennet produced using an expression vector of claim 1.
Claim 4
Independent Claim
51 words
A method of producing microbial rennet which comprises transfecting a microbial host with an expression vector having essentially the characteristics of any one of the plasmids pRl, pRlA, pRlX, pRlAX, pR2, pR2A, pR2X and pR3, culturing the host cells to produce the rennet, and isolating the rennet from the culture medium.
Claim 5
Independent Claim
51 words
A method of producing microbial rennet which comprises transfecting a microbial host with an expression vector having essentially the characteristics of any one of the plasmids pRl, pRlA, pRlX, pRlAX, pR2, pR2A, pR2X and pR3, culturing the host cells to produce the rennet, and isolating the rennet from the culture medium.
Claim 6
Dependent Claim
20 words
A process for obtaining rennin or prorennin by culturing cells containing recombinant DNA genetic material expressing rennin or prorennin respectively.
Claim 7
Dependent Claim
20 words
A process for obtaining rennin or prorennin by culturing cells containing recombinant DNA genetic material expressing rennin or prorennin respectively.
Claim 8
Dependent Claim
8 words
Rennin or prorennin produced by recombinant DNA techniques.
Claim 9
Dependent Claim
8 words
Rennin or prorennin produced by recombinant DNA techniques.
References Cited (Backward Citations)
EP0057350A2
Cited
EP0073029A2
Cited
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.