Patent US4652518A successfully refreshed from Google Patents.
US4652518A
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US
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Diagnosing chlamydia infections with Re-lipopolysaccharide complexed to carrier or antibody thereto
Assignee: Orion Oyj
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Filing: 1983-06-24
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Granted: 1987-03-24
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Expires: 2003-06-24
Abstract
Abstract A preparation for the detection of chlamydial infections using lipopolysaccharide of Re-lipopolysaccharide mutants of gram-negative bacteria. The lipopolysaccharide preparation is used in the production of group-specific antibodies to chlamydiae for diagnostic purposes or for the demonstration of antibodies to chlamydial group antigen in specimens.
Inventors
- 👤 Pirjo H. Makela
- 👤 Maija K. Leinonen
- 👤 Marjatta H. Nurminen-Kalliokoski
- 👤 Pekka A. I. Saikku
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 21:44
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56911; G01N33/56927; G01N2333/00; G01N2333/195; G01N2333/295; G01N2400/00; G01N2400/10; G01N2400/50; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/879; Y10S436/00; Y10S436/811; Y10S436/823
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
62 words
A preparation for diagnosing chlamydial infections which comprises a Re-lipopolysaccharide complexed to a carrier molecule to enhance immunological response, said Re-lipopolysaccharide being isolated from a Re-lipopolysaccharide mutant of a gram-negative bacterium, wherein said Re-lipopolysaccharide is capable of cross-reacting with chlamydial group-specific antibody as may be present in a tested patient fluid sample and thereby indicating the presence of such chlamydial group-specific antibody.
Claim 2
Dependent Claim
62 words
A preparation for diagnosing chlamydial infections which comprises a Re-lipopolysaccharide complexed to a carrier molecule to enhance immunological response, said Re-lipopolysaccharide being isolated from a Re-lipopolysaccharide mutant of a gram-negative bacterium, wherein said Re-lipopolysaccharide is capable of cross-reacting with chlamydial group-specific antibody as may be present in a tested patient fluid sample and thereby indicating the presence of such chlamydial group-specific antibody.
Claim 3
Dependent Claim
54 words
A preparation according to claim 1, wherein the Re-lipopolysaccharide comprises a lipopolysaccharide having a lipid A component and a KDO-oligosaccharide component containing two or three KDO-molecules, said Re-lipopolysaccharide having the structure ##STR4## wherein the structures of lipid A and R vary in bacterial strains and wherein the structure of the KDO-oligosaccharide remains fairly constant.
Claim 4
Dependent Claim
26 words
A preparation according to claim 2, wherein the product represented as (I) is treated with a weak alkali and is capable of cross-reacting with chlamydial glycolipid.
Claim 5
Dependent Claim
13 words
A preparation according to claim 1, wherein the carrier molecule is a protein.
Claim 6
Dependent Claim
13 words
A preparation according to claim 4, wherein the protein carrier molecule is porin.
Claim 7
Independent Claim
80 words
A method for detecting chlamydial infections, said method comprising the steps of: (a) contacting a given amount of a Re-lipopolysaccharide or an anti-Re-lipopolysaccharide antibody, said Re-lipopolysaccharide being isolated from a Re-lipopolysaccharide mutant of gram-negative bacteria, said anti-Re-lipopolysaccharide antibody being produced by immunizing animals with Re-lipopolysaccharide, with a sample to be assayed, and (b) assaying the contacted sample to determine whether the sample contains chlamydial group-specific antibody which cross-reacts with the Re-lipopolysaccharide or chlamydial group-specific antigen which cross-reacts with anti-Re-lipopolysaccharide antibody.
Claim 8
Independent Claim
80 words
A method for detecting chlamydial infections, said method comprising the steps of: (a) contacting a given amount of a Re-lipopolysaccharide or an anti-Re-lipopolysaccharide antibody, said Re-lipopolysaccharide being isolated from a Re-lipopolysaccharide mutant of gram-negative bacteria, said anti-Re-lipopolysaccharide antibody being produced by immunizing animals with Re-lipopolysaccharide, with a sample to be assayed, and (b) assaying the contacted sample to determine whether the sample contains chlamydial group-specific antibody which cross-reacts with the Re-lipopolysaccharide or chlamydial group-specific antigen which cross-reacts with anti-Re-lipopolysaccharide antibody.
Claim 9
Dependent Claim
19 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is treated with a weak alkali.
Claim 10
Dependent Claim
27 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is used for the demonstration of chlamydial group-specific antibody by serological and immunological methods.
Claim 11
Dependent Claim
34 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is treated with a weak alkali and is used for the demonstration of chlamydial group-specific antibody by serological and immunological methods.
Claim 12
Dependent Claim
26 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is used for the detection of chlamydial group-specific antibody by enzyme immunoassay methods.
Claim 13
Dependent Claim
33 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is treated with a weak alkali and is used for the detection of chlamydial group-specific antibody by enzyme immunoassay methods.
Claim 14
Dependent Claim
25 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is used for the detection of chlamydial group-specific antibody by counterimmunoelectrophoresis methods.
Claim 15
Dependent Claim
32 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is treated with a weak alkali and is used for the detection of chlamydial group-specific antibody by counterimmunoelectrophoresis methods.
Claim 16
Dependent Claim
26 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is used for the detection of chlamydial group-specific antibody by double immunodiffusion methods.
Claim 17
Dependent Claim
33 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is treated with a weak alkali and is used for the detection of chlamydial group-specific antibody by double immunodiffusion methods.
Claim 18
Dependent Claim
26 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is used for the detection of chlamydial group-specific antibody by passive hemagglutination methods.
Claim 19
Dependent Claim
33 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is treated with a weak alkali and is used for the detection of chlamydial group-specific antibody by passive hemagglutination methods.
Claim 20
Dependent Claim
27 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is used for the detection of chlamydial group-specific antibody by hemolysis in gel methods.
Claim 21
Dependent Claim
34 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is treated with a weak alkali and is used for the detection of chlamydial group-specific antibody by hemolysis in gel methods.
Claim 22
Dependent Claim
26 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is used for the detection of chlamydial group-specific antibody by latex agglutination methods.
Claim 23
Dependent Claim
33 words
A method for detecting chlamydial infections according to claim 6, wherein the Re-lipopolysaccharide is treated with a weak alkali and is used for the detection of chlamydial group-specific antibody by latex agglutination methods.
Claim 24
Dependent Claim
78 words
A preparation for diagnosing chlamydial infections which comprises a antibody to the complex of a Re-lipopolysaccharide and a carrier molecule to enhance immunological response, said anti-Re-lipopolysaccharide complex antibody being produced by immunizing animals with Re-lipoplysaccharide complexed to a carrier molecule to enhance immunological response, and wherein said anti-Re-lipopolysaccharide complex antibody is capable of cross-reacting with chlamydial group-specific antigen as may be present in a tested patient fluid sample and thereby indicating the presence of such chlamydial group-specific antigen.
Claim 25
Dependent Claim
78 words
A preparation for diagnosing chlamydial infections which comprises a antibody to the complex of a Re-lipopolysaccharide and a carrier molecule to enhance immunological response, said anti-Re-lipopolysaccharide complex antibody being produced by immunizing animals with Re-lipoplysaccharide complexed to a carrier molecule to enhance immunological response, and wherein said anti-Re-lipopolysaccharide complex antibody is capable of cross-reacting with chlamydial group-specific antigen as may be present in a tested patient fluid sample and thereby indicating the presence of such chlamydial group-specific antigen.
Claim 26
Dependent Claim
54 words
A preparation according to claim 22, wherein the Re-lipopolysaccharide comprises a lipopolysaccharide having a lipid A component and a KDO-oligosaccharide component containing two or three KDO-molecules, said Re-lipopolysaccharide having the structure ##STR5## wherein the structures of lipid A and R vary in bacterial strains and wherein the structure of the KDO-oligosaccharide remains fairly constant.
Claim 27
Dependent Claim
26 words
A preparation according to claim 23, wherein the product represented as (I) is treated with a weak alkali and is capable of cross-reacting with chlamydial glycolipid.
Claim 28
Dependent Claim
13 words
A preparation according to claim 22, wherein the carrier molecule is a protein.
Claim 29
Dependent Claim
13 words
A preparation according to claim 25, wherein the protein carrier molecule is porin.
References Cited (Backward Citations)
US4039657A
Cited
US4118469A
Cited
US4096035A
Cited
EP0017460A1
Cited
GB2047889A
Cited
US4310455A
Cited
US4267170A
Cited
US4271146A
Cited
EP0050424A1
Cited
US4397959A
Cited
US4497899A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-03-23 | 1990-09-22 | 1991-03-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-03-23 | 1994-09-22 | 1995-03-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-03-23 | 1998-09-22 | 1999-03-24 | $7700.00 | Window Closed / Lapsed |