US4118469A
Expired / Lapsed
US
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Antigen for trachoma lymphogranuloma venereum (LGV) and non-gonococcal urethritis (NGU)
Assignee: Research Corp
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Filing: 1976-04-27
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Granted: 1978-10-03
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Expires: 1996-04-27
Abstract
Abstract Solubilized antigens of C. trachomatis strain LGV-434 upon analysis using two-dimensional immunoelectrophoresis yielded a single antigen which was found to be consistently precipitated by sera of patients with C. trachomatis infections. This antigen as antigen-antibody complex was employed as an immunogen to prepare a rabbit monospecific antiserum to this component or antigen. This monospecific antiserum demonstrated the presence of the antigen in each of the 15 strains of C. trachomatis organisms and was non-reactive with strains of C. psittaci. The C. trachomatis specific antigen was purified by immunoadsorption chromatography using monospecific antiserum as a specific ligand covalently bound to agarose gel columns and the resulting purified antigen employed to detect antibody from the sera of lymphogranuloma venereum patients using counterimmunoelectrophoresis. When the C. trachomatis specific antigen is isotopically labeled and utilized in the highly sensitive radioimmune assay antibody to the antigen should be demonstrated and a serological test based thereon should be applicable for the serological diagnosis of non-gonococcal urethritis (NGU).
Inventors
- 👤 Harlan D. Caldwell
- 👤 Cho-Chou Kuo
- 👤 George E. Kenny
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 10:10
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/118; C; C07; C07K; C07K16/00; C07K16/12; C07K16/1203; C07K16/125; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/571; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/805; Y10S530/00; Y10S530/806; Y10S530/82; Y10S530/823
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
55 words
Chlamydia trachomatis specific antigen useful for the serological diagnosis of lymphogranuloma venereum, said antigen having a molecular weight of about 160,000, being stable to ribonuclease and deoxyribonuclease and at a pH in the range 2.2-10.6 but whose antigenicity is destroyed by protease treatment and heating at a temperature of 56° C. for about 30 minutes.
Claim 2
Dependent Claim
55 words
Chlamydia trachomatis specific antigen useful for the serological diagnosis of lymphogranuloma venereum, said antigen having a molecular weight of about 160,000, being stable to ribonuclease and deoxyribonuclease and at a pH in the range 2.2-10.6 but whose antigenicity is destroyed by protease treatment and heating at a temperature of 56° C. for about 30 minutes.
Claim 3
Dependent Claim
10 words
Radioactive isotopically labeled Chlamydia trachomatis specific antigen of claim 1.
Claim 4
Dependent Claim
28 words
An aqueous composition comprising the antigen of claim 1, said composition having a pH in the range from about 7 to about 8.6 suitable for low temperature storage.
Claim 5
Dependent Claim
28 words
An aqueous composition comprising the antigen of claim 2, said composition having a pH in the range from about 7 to about 8.6 suitable for low temperature storage.
Claim 6
Independent Claim
55 words
A method of preparing Chlamydia trachomatis antigen which is consistently precipitated by sera of patients with C. trachomatis infections which comprises solubilizing C. trachomatis in the presence of a non-ionic surfactant and recovering the resulting solubilized C. trachomatis antigen by immunoadsorption chromatography employing monospecific antiserum to said antigen as a specific ligand for said antigen.
Claim 7
Independent Claim
55 words
A method of preparing Chlamydia trachomatis antigen which is consistently precipitated by sera of patients with C. trachomatis infections which comprises solubilizing C. trachomatis in the presence of a non-ionic surfactant and recovering the resulting solubilized C. trachomatis antigen by immunoadsorption chromatography employing monospecific antiserum to said antigen as a specific ligand for said antigen.
Claim 8
Dependent Claim
22 words
A method in accordance with claim 5 wherein said non-ionic surfactant is a 1% (volume/volume) solution of an alkyl aryl polyether alcohol.
Claim 9
Independent Claim
34 words
A method of isolating Chlamydia trachomatis antigen in the form of its antigen-antibody complex which comprises solubilizing C. trachomatis antigen and subjecting the resulting solubilized antigen to two-dimensional immunoelectrophoresis and recovering the antigen-antibody complex.
Claim 10
Independent Claim
34 words
A method of isolating Chlamydia trachomatis antigen in the form of its antigen-antibody complex which comprises solubilizing C. trachomatis antigen and subjecting the resulting solubilized antigen to two-dimensional immunoelectrophoresis and recovering the antigen-antibody complex.
Claim 11
Dependent Claim
20 words
A method in accordance with claim 7 wherein said C. trachomatis antigen is solubilized by means of an non-ionic surfactant.
Claim 12
Independent Claim
74 words
A method of preparing monospecific antiserum to antigen of Chamydia trachomatis which comprises solubilizing the antigens of C. trachomatis in the presence of a non-ionic surfactant, subjecting the resulting solubilized antigens to two-dimensional immunoelectrophoresis on agarose, thereby precipitating the C. trachomatis antigen as an antigen-antibody complex, recovering said antigen-antibody complex, introducing said resulting recovered antigen-antibody complex into rabbit, thereby causing the production of antiserum therein and recovering rabbit serum containing the resulting produced antiserum.
Claim 13
Independent Claim
74 words
A method of preparing monospecific antiserum to antigen of Chamydia trachomatis which comprises solubilizing the antigens of C. trachomatis in the presence of a non-ionic surfactant, subjecting the resulting solubilized antigens to two-dimensional immunoelectrophoresis on agarose, thereby precipitating the C. trachomatis antigen as an antigen-antibody complex, recovering said antigen-antibody complex, introducing said resulting recovered antigen-antibody complex into rabbit, thereby causing the production of antiserum therein and recovering rabbit serum containing the resulting produced antiserum.
Claim 14
Dependent Claim
61 words
A method for the serological testing of antibody of Chlamydia trachomatis which comprises subjecting serum of suspected lymphogranuloma venereum patients to counterimmunoelectrophoresis on agarose employing the antigen of claim 1 in said counterimmunoelectrophoresis, observing during said counter immunoelectrophoresis for the presence of resulting antigen-antibody complex, and detecting said complex by staining said agarose containing said antigen-antibody complex with a suitable dye.
Claim 15
Dependent Claim
40 words
A method for the serological testing of antibody to Chlamydia trachomatis which comprises subjecting serum of suspected non-gonococcal urethritis patients to radioimmune assay employing the radioactive isotopically labeled antigen of claim 2 in order to detect formation of antigen-antibody complex.
Claim 16
Dependent Claim
9 words
A lyophilized composition containing the antigen of claim 1.
Claim 17
Dependent Claim
12 words
A lyophilized composition containing a radioactive isotopically labeled antigen of claim 2.
Claim 18
Dependent Claim
16 words
A stable composition containing the antigen of claim 1 at a concentration of about 30 μg/ml.
Claim 19
Dependent Claim
54 words
An agarose coated inert substrate, the agarose coating on said substrate being provided with at least two wells cut thereinto, one of said wells having deposited therein the antigen of claim 1 and another of said wells being suitable for the placement therein of serum from a patient for the diagnosis of lymphogranuloma venereum.
Claim 20
Dependent Claim
24 words
An agarose coated inert substrate in accordance with claim 15 wherein said well has antigen deposited therein in an amount of about 60 ηg.
Claim 21
Dependent Claim
17 words
An agarose coated substrate in accordance with claim 15 wherein said inert substrate is a glass slide.
Claim 22
Dependent Claim
35 words
An agarose coated inert substrate in accordance with claim 15 wherein said agarose coating comprises an non-ionic detergent together with a barbital buffer, said agarose coating having been applied at a pH of about 8.6.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-10-02 | 1982-04-03 | 1982-10-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-10-02 | 1986-04-03 | 1986-10-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-10-02 | 1990-04-03 | 1990-10-03 | $7700.00 | Window Closed / Lapsed |