Patent US2412833A successfully refreshed from Google Patents.
US4497899A
Expired / Lapsed
US
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Immunoassay for Chlamydia trachomatis antigens
Assignee: Abbott Laboratories
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Filing: 1983-03-28
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Granted: 1985-02-05
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Expires: 2003-03-28
Abstract
Abstract The present disclosure relates to a solid phase immunoassay for the detection of Chlamydia trachomatis antigens in a clinical specimen, wherein the Chlamydia trachomatis antigens to be determined are coated or adsorbed on the solid phase.
Inventors
- 👤 Alan S. Armstrong
- 👤 John E. Herrmann
- 👤 Lawrence V. Howard
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 22:46
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56911; G01N33/56927; Y; Y10; Y10S; Y10S435/00; Y10S435/962; Y10S436/00; Y10S436/804; Y10S436/811; Y10S436/815; Y10S436/823; Y10S436/825; Y10S436/826
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
133 words
A method for determining Chlamydia trachomatis antigen in a clinical specimen comprising: (a) lysing Chlamydia trachomatis cells in the specimen to release Chlamydia trachomatis antigen; (b) coating a bare solid support with Chlamydia trachomatis antigen in the specimen; (c) separating the antigen coated solid support from the specimen; (d) treating the antigen coated solid support with chlamydial antibody to form an antigen-chlamydial antibody complex on the solid support; (e) separating the antigen-chlamydial antibody complex from unbound chlamydial antibody; (f) treating the antigen-chlamydial antibody complex with a labeled antiglobulin to form an antigen-chlamydial antibody-labeled antiglobulin complex on the solid support; (g) separating the antigen-chlamydial antibody-labeled antiglobulin complex from unbound labeled antiglobulin; and (h) determining the labeled antiglobulin bound to the antigen-chlamydial antibody complex as a measure of the Chlamydia trachomatis antigen in the specimen.
Claim 2
Independent Claim
133 words
A method for determining Chlamydia trachomatis antigen in a clinical specimen comprising: (a) lysing Chlamydia trachomatis cells in the specimen to release Chlamydia trachomatis antigen; (b) coating a bare solid support with Chlamydia trachomatis antigen in the specimen; (c) separating the antigen coated solid support from the specimen; (d) treating the antigen coated solid support with chlamydial antibody to form an antigen-chlamydial antibody complex on the solid support; (e) separating the antigen-chlamydial antibody complex from unbound chlamydial antibody; (f) treating the antigen-chlamydial antibody complex with a labeled antiglobulin to form an antigen-chlamydial antibody-labeled antiglobulin complex on the solid support; (g) separating the antigen-chlamydial antibody-labeled antiglobulin complex from unbound labeled antiglobulin; and (h) determining the labeled antiglobulin bound to the antigen-chlamydial antibody complex as a measure of the Chlamydia trachomatis antigen in the specimen.
Claim 3
Dependent Claim
20 words
A method according to claim 1 wherein the Chlamydia trachomatis antigen is lysed using buffered saline solution and a surfactant.
Claim 4
Dependent Claim
13 words
A method according to claim 2 wherein the surfactant is a deoxycholate salt.
Claim 5
Dependent Claim
12 words
A method according to claim 3 wherein the deoxycholate salt is chenodeoxycholate.
Claim 6
Dependent Claim
20 words
A method according to claim 4 wherein a reducing agent is added to the buffered saline solution and the surfactant.
Claim 7
Dependent Claim
17 words
A method according to claim 5 wherein the reducing agent is dithiothreitol and the surfactant is chenodeoxycholate.
Claim 8
Dependent Claim
14 words
A method according to claim 1 wherein the antiglobulin is labeled with an enzyme.
Claim 9
Independent Claim
162 words
A method for determining Chlamydia trachomatis antigen in a clinical specimen comprising: (a) lysing Chlamydia trachomatis cells in the specimen to release Chlamydia trachomatis antigen; (b) coating a bare solid support with the lysed Chlamydia trachomatis antigen; (c) separating the antigen coated solid support from the specimen; (d) treating the antigen coated solid support with chlamydial antibody to form an antigen-chlamydial antibody complex on the solid support; (e) separating the antigen-chlamydial antibody complex from unbound chlamydial antibody; (f) treating the antigen-chlamydial antibody complex with antiglobulin, to form an antigen-chlamydial antibody-antiglobulin complex on the solid support; (g) separating the antigen-chlamydial antibody-antiglobulin complex from unbound antiglobulin; (h) treating the antigen-chlamydial antibody-antiglobulin complex with labeled antibody to the antiglobulin to form an antigen-chlamydial antibody antiglobulin-labeled antibody complex on the solid support; (i) separating the antigen-chlamydial antibody-antiglobulin-labeled antibody complex from unbound labeled antibody; and (j) determining the labeled antibody bound to the antigen-chlamydial antibody-antiglobulin complex as a measure of the Chlamydia trachomatis antigen in the specimen.
Claim 10
Independent Claim
162 words
A method for determining Chlamydia trachomatis antigen in a clinical specimen comprising: (a) lysing Chlamydia trachomatis cells in the specimen to release Chlamydia trachomatis antigen; (b) coating a bare solid support with the lysed Chlamydia trachomatis antigen; (c) separating the antigen coated solid support from the specimen; (d) treating the antigen coated solid support with chlamydial antibody to form an antigen-chlamydial antibody complex on the solid support; (e) separating the antigen-chlamydial antibody complex from unbound chlamydial antibody; (f) treating the antigen-chlamydial antibody complex with antiglobulin, to form an antigen-chlamydial antibody-antiglobulin complex on the solid support; (g) separating the antigen-chlamydial antibody-antiglobulin complex from unbound antiglobulin; (h) treating the antigen-chlamydial antibody-antiglobulin complex with labeled antibody to the antiglobulin to form an antigen-chlamydial antibody antiglobulin-labeled antibody complex on the solid support; (i) separating the antigen-chlamydial antibody-antiglobulin-labeled antibody complex from unbound labeled antibody; and (j) determining the labeled antibody bound to the antigen-chlamydial antibody-antiglobulin complex as a measure of the Chlamydia trachomatis antigen in the specimen.
Claim 11
Dependent Claim
24 words
A method according to claim 8 wherein the Chlamydia trachomatis antigen is lysed from the specimen using a buffered saline solution and a surfactant.
Claim 12
Dependent Claim
13 words
A method according to claim 9 wherein the surfactant is a deoxycholate salt.
Claim 13
Dependent Claim
20 words
A method according to claim 10 wherein a reducing agent is added to the buffered saline solution and the surfactant.
Claim 14
Dependent Claim
17 words
A method accoring to claim 11 wherein the reducing agent is dithiothreitol and the surfactant is chenodeoxycholate.
Claim 15
Dependent Claim
14 words
A method according to claim 8 wherein the antiglobulin is labeled with an enzyme.
Claim 16
Independent Claim
93 words
A method for determining Chlamydia trachomatis antigen in a clinical specimen comprising: (a) lysing the Chlamydia trachomatis antigen; (b) coating a bare solid support with the lysed Chlamydia trachomatis antigen; (c) separating the antigen coated solid support from the specimen; (d) treating the antigen coated solid support with labeled chlamydial antibody; (e) separating the antigen coated solid support with the labeled chlamydial antibody from unbound labeled chlamydial antibody; and (f) determining the labeled chlamydial antibody bound to the antigen coated solid support as a measure of the Chlamydia trachomatis antigen in the specimen.
Claim 17
Independent Claim
93 words
A method for determining Chlamydia trachomatis antigen in a clinical specimen comprising: (a) lysing the Chlamydia trachomatis antigen; (b) coating a bare solid support with the lysed Chlamydia trachomatis antigen; (c) separating the antigen coated solid support from the specimen; (d) treating the antigen coated solid support with labeled chlamydial antibody; (e) separating the antigen coated solid support with the labeled chlamydial antibody from unbound labeled chlamydial antibody; and (f) determining the labeled chlamydial antibody bound to the antigen coated solid support as a measure of the Chlamydia trachomatis antigen in the specimen.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-02-05 | 1988-08-06 | 1989-02-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-02-05 | 1992-08-06 | 1993-02-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-02-05 | 1996-08-06 | 1997-02-05 | $7700.00 | Window Closed / Lapsed |