US4039657A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Separating antigen from residual common sensitizing protein
Assignee: Merck and Co Inc
•
Filing: 1976-04-12
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Granted: 1977-08-02
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Expires: 1996-04-12
Abstract
Abstract Sensitizing plasma protein is dissociated from antigen-plasma protein complex by agitating at low temperature under controlled pH.
Inventors
- 👤 William J. McAleer
- 👤 Edward H. Wasmuth
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 21:23
CPC Classifications
A; A61; A61K; A61K39/00
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
73 words
A process for separating biological immunizing antigen having a particle size of from about 10 nm to about 10 μm from serum derived residual common sensitizing protein components which comprises agitating the biological antigen containing residual common sensitizing protein in phosphate buffered saline having a pH of from about 6.8 to about 7.6 at a temperature of from about 0° C. to about 10° C. for from about 48 to about 96 hours.
Claim 2
Dependent Claim
73 words
A process for separating biological immunizing antigen having a particle size of from about 10 nm to about 10 μm from serum derived residual common sensitizing protein components which comprises agitating the biological antigen containing residual common sensitizing protein in phosphate buffered saline having a pH of from about 6.8 to about 7.6 at a temperature of from about 0° C. to about 10° C. for from about 48 to about 96 hours.
Claim 3
Dependent Claim
26 words
A process according to claim 1 followed by removal of the dissociated residual common sensitizing components from the antigen by mechanical removal in a closed system.
Claim 4
Dependent Claim
14 words
A process according to claim 2 wherein the mechanical removal is thin channel ultrafiltration.
Claim 5
Dependent Claim
17 words
The process of claim 1, wherein the agitating takes place at a temperature of about 5° C.
Claim 6
Dependent Claim
15 words
The process of claim 1, wherein the agitating is carried out for about 72 hours.
Claim 7
Dependent Claim
12 words
The process of claim 1, wherein the biological immunizing antigen is PPLO.
Claim 8
Dependent Claim
13 words
The process of claim 1, wherein the biological immunizing antigen is Trachoma agent.
Claim 9
Dependent Claim
18 words
The process of claim 6, wherein the agitating takes place at about 5° C. for about 72 hours.
Claim 10
Dependent Claim
18 words
The process of claim 7, wherein the agitating takes place at about 5° C. for about 72 hours.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-08-01 | 1981-01-31 | 1981-08-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-01 | 1985-01-31 | 1985-08-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-08-01 | 1989-01-31 | 1989-08-02 | $7700.00 | Window Closed / Lapsed |