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US4563425A
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Enzyme reaction method for isomerization of glucose to fructose
Assignee: Toray Industries Inc
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Filing: 1984-04-02
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Granted: 1986-01-07
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Expires: 2004-04-02
Abstract
Abstract A metal ion that inhibits enzyme deactivation is bonded to a carrier and inhibits enzyme deactivation when contacted with a substrate for the enzyme. A carrier-bound metal ion such as ions of iron is particularly suitable for inhibiting deactivation of glucose isomerase when isomerizing glucose to fructose. Glucose isomerase life is remarkably prolonged by contacting carrier-bound iron ions with a glucose substrate solution prior to isomerizing with glucose isomerase.
Inventors
- 👤 Toshio Yoshioka
- 👤 Kazuo Teramoto
- 👤 Masaharu Shimamura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 15:21
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/90; C12N9/92; C12P; C12P19/00; C12P19/24
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
49 words
In an enzyme isomerization reaction method in which a glucose-containing substrate solution is contacted with an immobilized glucose isomerase to produce a fructose-containing syrup, the improvement comprising, prior to the isomerization reaction, contacting said substrate solution with a synthetic organic ion exchanger to which ions of iron are bonded.
Claim 2
Dependent Claim
49 words
In an enzyme isomerization reaction method in which a glucose-containing substrate solution is contacted with an immobilized glucose isomerase to produce a fructose-containing syrup, the improvement comprising, prior to the isomerization reaction, contacting said substrate solution with a synthetic organic ion exchanger to which ions of iron are bonded.
Claim 3
Dependent Claim
21 words
An enzyme reaction method according to claim 1, wherein said substrate solution is passed through layers filled with said ion exchanger.
Claim 4
Dependent Claim
25 words
An enzyme reaction method according to claim 1, wherein said immobilized glucose isomerase is formed by immobilizing an isomerase-containing fungus body on an insolubilized material.
Claim 5
Dependent Claim
43 words
An enzyme reaction method according to claim 3, wherein said insolubilized material is selected from the group consisting of quaternary vinyl pyridine resin, DEAE-cellulose, porous alumina, polyphenolic anion exchange resin, styrene-divinylbenzene-based macro-reticular or porous anion exchange resin, anion exchange fiber and mixtures thereof.
Claim 6
Dependent Claim
65 words
An enzyme reaction method according to claim 1, wherein a solution containing 30 to 60% by weight of glucose and 0.1˜20 mM/l of magnesium ions is passed through at least one fixed bed composed of first-stage layers packed with said synthetic organic ion exchanger containing said bound iron ions and then through second-stage layers of said immobilized glucose isomerase at a temperature of 55°-70° C.
Claim 7
Dependent Claim
16 words
An enzyme reaction method according to claim 1, wherein said synthetic organic ion exchanger is styrene-based.
Claim 8
Dependent Claim
47 words
An enzyme reaction method according to claim 6, wherein said ion exchanger has at least one ion exchange group selected from the group consisting of amine, carboxylic acid, phosphoric acid, sulfonic acid, hydroxyl, carboxyl, polycarboxylic acid, aldehyde, polyamino, amino carboxylic acid, peptide, β-diketone groups and mixtures thereof.
Claim 9
Dependent Claim
35 words
An enzyme reaction method according to claim 7, wherein said ion exchange group is selected from the group consisting of amino, carboxylic acid, phosphoric acid, sulfonic acid, polyamino, amino carboxylic acid groups and mixtures thereof.
Claim 10
Dependent Claim
53 words
An enzyme reaction method according to claim 8, wherein said polyamino group is selected from the group consisting of --NH(C 2 H 4 NH) n H (where n is a number of 1 or greater), --NH(CH 2 ) m NH 2 (where m is a number of 3 to 12) and mixtures thereof.
Claim 11
Dependent Claim
30 words
An enzyme reaction method according to claim 8, wherein said amino carboxylic acid group is selected from the group consisting of iminodiacetic acid group, polyaminopolyacetic acid group and mixtures thereof.
Claim 12
Dependent Claim
24 words
An enzyme reaction method according to claim 1, wherein the amount of said iron ions is at least 0.01 meq/g of said ion exchanger.
Claim 13
Dependent Claim
24 words
An enzyme reaction method according to claim 11, wherein the amount of said iron ions is greater than 0.1 meq/g of said ion exchanger.
Claim 14
Dependent Claim
29 words
An enzyme reaction method according to claim 11, wherein the amount of said iron ions is within the range of from 0.5 to 2.5 meq/g of said ion exchanger.
Claim 15
Dependent Claim
35 words
An enzyme reaction method according to claim 1, wherein said ion exchanger is in a form selected from the group consisting of powder, fine grains, resinous grains, fibers, hollow fibers, film, paper and mixtures thereof.
Claim 16
Dependent Claim
20 words
An enzyme reaction method according to claim 14, wherein said resinous grains are selected from macro-reticular and porous resinous grains.
Claim 17
Dependent Claim
18 words
An enzyme reaction method according to claim 14, wherein said fibers are composed of a fiber forming polymer.
Claim 18
Dependent Claim
31 words
An enzyme reaction method according to claim 16, wherein said fiber forming polymer is at least one polymer selected from the group consisting of polyamide, polyester, poly-α-olefine, polyacrylonitrile and copolymers thereof.
Claim 19
Dependent Claim
24 words
An enzyme reaction method according to claim 1, wherein said immobilized glucose isomerase is formed by immobilizing an extracted isomerase on an insolubilized material.
Claim 20
Dependent Claim
23 words
An enzyme reaction method according to claim 1, wherein said immobilized glucose isomerase is formed by crosslinking an isomerase-containing fungus body with glutaraldehyde.
Claim 21
Dependent Claim
29 words
An enzyme reaction method according to claim 1, wherein said immobilized glucose isomerase is formed by incorporating extracted isomerase in cellulose acetate and spinning the mixture into a fiber.
Claim 22
Dependent Claim
53 words
An enzyme reaction method according to claim 1, wherein said immobilized glucose isomerase is formed by a method selected from the group consisting of immobilizing an extracted isomerase on an insolubilized material, crosslinking an isomerase-containing fungus body with glutaraldehyde, and incorporating extracted isomerase in cellulose acetate and spinning the mixture into a fiber.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1989-01-06 | 1989-07-08 | 1990-01-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-01-06 | 1993-07-08 | 1994-01-07 | $3760.00 | Window Closed / Lapsed |
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