US4263400A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilized enzymes
Assignee: Genencor International Wisconsin Inc
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Filing: 1979-01-11
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Granted: 1981-04-21
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Expires: 1999-01-11
Abstract
Abstract When immobilizing enzymes such as glucose isomerase by adsorption on a carrier such as an ion exchange resin, the enzyme prior to adsorption on the carrier is separated from a high molecular weight, non-dialyzable polysaccharide which inhibits adsorption of the enzyme to the carrier.
Inventors
- 👤 Soichiro Ushiro
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 02:27
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/90; C12N9/92; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12P; C12P19/00; C12P19/24; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
111 words
A method for the production of immobilized glucose isomerase comprising: (a) obtaining a crude glucose isomerase preparation containing a high molecular weight, nondialyzable polysaccharide which inhibits adsorption of said glucose isomerase on an ion exchange resin from a microorganism which produces and retains the glucose isomerase substantially intracellularly; (b) separating said polysaccharide from said glucose isomerase preparation to give a purified glucose isomerase essentially completely free of said polysaccharide; and then (c) contacting a solution of the purified glucose isomerase with an ion exchange resin to absorb and immobilize said glucose isomerase thereon, said ion exchange resin being one capable of adsorbing said polysaccharide which inhibits adsorption of said glucose isomerase.
Claim 2
Independent Claim
111 words
A method for the production of immobilized glucose isomerase comprising: (a) obtaining a crude glucose isomerase preparation containing a high molecular weight, nondialyzable polysaccharide which inhibits adsorption of said glucose isomerase on an ion exchange resin from a microorganism which produces and retains the glucose isomerase substantially intracellularly; (b) separating said polysaccharide from said glucose isomerase preparation to give a purified glucose isomerase essentially completely free of said polysaccharide; and then (c) contacting a solution of the purified glucose isomerase with an ion exchange resin to absorb and immobilize said glucose isomerase thereon, said ion exchange resin being one capable of adsorbing said polysaccharide which inhibits adsorption of said glucose isomerase.
Claim 3
Dependent Claim
16 words
The method of claim 1, wherein the glucose isomerase preparation is obtained from a Streptomyces microorganism.
Claim 4
Dependent Claim
11 words
The method of claim 2, wherein the microorganism is Streptomyces olivochromogenes.
Claim 5
Dependent Claim
34 words
The method of claim 1, wherein the purified glucose isomerase essentially completely free of said polysacchoride is prepared by contacting the crude preparation with an ion exchange resin which selectively adsorbs and removes polysaccharide.
Claim 6
Dependent Claim
40 words
The method of claim 4, wherein the ion exchange resin is selected from the group consisting of a strongly-basic anion exchange resin (type I); strongly-basic anion exchange resin (type II); moderately-basic anion exchange resin, or a weakly-basic anion exchange resin.
Claim 7
Dependent Claim
53 words
The method of claim 1, wherein the purified glucose isomerase essentially completely free of said polysaccharide is prepared by combining the crude preparation with an organic solvent at a concentration at which the glucose isomerase will be selectively precipitated to separate said glucose isomerase from said polysaccharide, and then redissolving said glucose isomerase.
Claim 8
Dependent Claim
26 words
The method of claim 6, wherein the organic solvent is selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, acetone, p-dioxane and admixtures thereof.
Claim 9
Dependent Claim
52 words
The method of claim 1, wherein the purified glucose isomerase essentially completely free of said polysaccharide is obtained by combining the crude preparation with a salt at a concentration at which the glucose isomerase will be selectively precipitated to separate said glucose isomerase from said polysaccharide, and then redissolving said glucose isomerase.
Claim 10
Dependent Claim
24 words
The method of claim 8, wherein the salt is selected from the group consisting of ammonium sulfate, sodium sulfate, magnesium sulfate and admixtures thereof.
Claim 11
Dependent Claim
11 words
The immobilized glucose isomerase produced by the method of claim 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-04-20 | 1984-10-20 | 1985-04-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-04-20 | 1988-10-20 | 1989-04-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-04-20 | 1992-10-20 | 1993-04-21 | $7700.00 | Window Closed / Lapsed |