US4208482A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilization of glucose isomerase
Assignee: Busch Industrial Products Corp
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Filing: 1976-04-23
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Granted: 1980-06-17
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Expires: 1996-04-23
Abstract
Abstract Immobilized glucose isomerase is prepared by mixing 0.5 to 1.5 parts by weight whole microbial cells containing glucose isomerase with one part by weight agar, combining the resultant mixture with an organic solvent, recovering discrete particles of agar gel with the whole microbial cells entrapped therein and drying the agar gel particles. The immobilized glucose isomerase has long half life stability when used in a column in a continuous process to convert glucose to fructose.
Inventors
- 👤 Irving Ehrenthal
- 👤 Keith E. Miner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 04:59
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/04; C12P; C12P19/00; C12P19/24
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
70 words
A process of immobilizing glucose isomerase comprising the steps of: A. Mixing 0.5 to 1.5 parts by weight whole microbial cells containing glucose isomerase with one part by weight of agar, B. Combining the agar-microbial cell mixture with organic solvent, C. Recovering discrete particles of agar gel with whole microbial cells containing glucose isomerase dispersed therethrough, and D. Drying the particles to a moisture level of less than about 15%.
Claim 2
Dependent Claim
70 words
A process of immobilizing glucose isomerase comprising the steps of: A. Mixing 0.5 to 1.5 parts by weight whole microbial cells containing glucose isomerase with one part by weight of agar, B. Combining the agar-microbial cell mixture with organic solvent, C. Recovering discrete particles of agar gel with whole microbial cells containing glucose isomerase dispersed therethrough, and D. Drying the particles to a moisture level of less than about 15%.
Claim 3
Dependent Claim
24 words
The process of claim 1 wherein the ratio of whole microbial cells containing glucose isomerase to agar is about 1 to 1 by weight.
Claim 4
Dependent Claim
18 words
The process of claim 1 wherein said organic solvent is a mixture of ethyl acetate and ethylene dichloride.
Claim 5
Dependent Claim
18 words
The process of claim 3 wherein the ratio of ethyl acetate to ethylene dichloride is 3 to 1.
Claim 6
Dependent Claim
12 words
The process of claim 1 wherein said organic solvent is ethyl acetate.
Claim 7
Dependent Claim
27 words
The process of claim 1 wherein the organic solvent is at a temperature of between 5° C. and 15° C. when the agar-enzyme mix is combined therewith.
Claim 8
Dependent Claim
13 words
The process of claim 1 wherein the particles are substantially spherical in shape.
Claim 9
Dependent Claim
12 words
The process of claim 1 wherein said organic solvent is butyl acetate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-06-17 | 1983-12-17 | 1984-06-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-06-17 | 1987-12-17 | 1988-06-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-06-17 | 1991-12-17 | 1992-06-17 | $7700.00 | Window Closed / Lapsed |