US4246350A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Protein immobilization on chelating resins
Assignee: Dow Chemical Co
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Filing: 1979-03-01
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Granted: 1981-01-20
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Expires: 1999-03-01
Abstract
Abstract Proteins such as enzymes are immobilized on macroporous resins having recurring bis-picolylamine, imino diacetate or hydroxyethyl picolylamine chelating sites. Immobilization is carried out by contacting the resin with a multivalent metal ion selected from the transition metals or rare earth metals and adsorbing the protein to the resin. This immobilization technique permits strongly binding protein to a high surface area resin. The protein can be readily removed from the resin by simply flushing the resin with an appropriate solution.
Inventors
- 👤 Deborah E. Hier
- 👤 Patrick J. Oriel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 19:59
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/24; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
91 words
A process for immobilizing a protein on a macroporous styrene-divinylbenzene copolymer resin support having a pore size from about 50 angstroms to about 2000 angstroms in diameter and recurring bis-picolylamine, imino diacetate, or hydroxyethyl picolylamine chelating sites thereon which comprises a first step of contacting the resin support with a multivalent metal ion selected from the transition metals or rare earth metals whereby a chelate is formed between the multivalent metal ions and the recurring chelating sites on the copolymer and a second step of immobilizing the protein on the copolymer.
Claim 2
Dependent Claim
91 words
A process for immobilizing a protein on a macroporous styrene-divinylbenzene copolymer resin support having a pore size from about 50 angstroms to about 2000 angstroms in diameter and recurring bis-picolylamine, imino diacetate, or hydroxyethyl picolylamine chelating sites thereon which comprises a first step of contacting the resin support with a multivalent metal ion selected from the transition metals or rare earth metals whereby a chelate is formed between the multivalent metal ions and the recurring chelating sites on the copolymer and a second step of immobilizing the protein on the copolymer.
Claim 3
Dependent Claim
17 words
The process of claim 1 wherein the multivalent metal ions are selected from the rare earth metals.
Claim 4
Dependent Claim
56 words
A water-insoluble polymer-protein conjugate comprising a macroporous styrene-divinylbenzene copolymer resin support having a pore size from about 50 angstroms to about 2000 angstroms in diameter and active bis-picolylamine, imino diacetate, or hydroxyethyl picolylamine sites forming a chelate with a multivalent metal ion selected from the transition metals or rare earth metals and a protein adsorbed thereon.
Claim 5
Dependent Claim
56 words
A water-insoluble polymer-protein conjugate comprising a macroporous styrene-divinylbenzene copolymer resin support having a pore size from about 50 angstroms to about 2000 angstroms in diameter and active bis-picolylamine, imino diacetate, or hydroxyethyl picolylamine sites forming a chelate with a multivalent metal ion selected from the transition metals or rare earth metals and a protein adsorbed thereon.
Claim 6
Dependent Claim
17 words
The conjugate of claim 3 wherein the multivalent metal ion is selected from the rare earth metals.
Claim 7
Dependent Claim
25 words
The conjugate of claim 3 wherein the pore size of the macroporous resin is from about 200 angstroms and to about 2000 angstroms in diameter.
Claim 8
Dependent Claim
13 words
The conjugate of claims 3 or 6 wherein the protein is an enzyme.
Claim 9
Dependent Claim
12 words
Conjugate of claim 3 or 6 wherein the protein is glucose isomerase.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-01-20 | 1984-07-21 | 1985-01-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-01-20 | 1988-07-21 | 1989-01-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-01-20 | 1992-07-21 | 1993-01-20 | $7700.00 | Window Closed / Lapsed |