US3990943A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the enzymatic isomerization of glucose to levulose
Assignee: Rhone Poulenc SA
•
Filing: 1974-09-25
•
Granted: 1976-11-09
•
Expires: 1994-09-25
Abstract
Abstract A process for the enzymatic isomerization of glucose to fructose where the glucose isomerase is combined with magnesium or magnesium and cobalt ions which have been fixed to a cation exchange resin. The glucose isomerase remains combined with the metal ions during the isomerization. The use of the instant complex prolongs the working life of the enzyme and minimizes the need for conventional pH control.
Inventors
- 👤 Albert Bouniot
- 👤 Michel Guerineau
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 22:26
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/24; C13; C13K; C13K11/00
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
96 words
In a process for the isomerisation of glucose to levulose which comprises treating an aqueous solution of glucose with a glucose isomerase in the presence of the activating cations Mg + + or Mg + + and Co + + , the improvement which consists in contacting a cation exchanger, having Mg + + or Mg + + and Co + + cations fixed thereto, with the glucose isomerase so that the said glucose isomerase becomes combined with the said fixed cations, and then treating the aqueous solution of glucose with the said combined glucose isomerase.
Claim 2
Dependent Claim
96 words
In a process for the isomerisation of glucose to levulose which comprises treating an aqueous solution of glucose with a glucose isomerase in the presence of the activating cations Mg + + or Mg + + and Co + + , the improvement which consists in contacting a cation exchanger, having Mg + + or Mg + + and Co + + cations fixed thereto, with the glucose isomerase so that the said glucose isomerase becomes combined with the said fixed cations, and then treating the aqueous solution of glucose with the said combined glucose isomerase.
Claim 3
Dependent Claim
23 words
The improvement of claim 1, in which said cation exchanger is a cation exchange resin carrying sulphonic acid groups or carboxylic acid groups.
Claim 4
Dependent Claim
48 words
The improvement of claim 2, in which said resin, initially in the acid form, is saturated with magnesium hydroxide, and then washed with a saturated aqueous solution of magnesium carbonate, before being mixed with the glucose isomerase or the remains of cells of a micro-organism containing glucose isomerase.
Claim 5
Dependent Claim
20 words
The improvement of claim 1, in which the concentration of the solution of glucose does not exceed 60% by weight.
Claim 6
Dependent Claim
24 words
The improvement of claim 1, in which the solution of glucose contains Mg + + or Mg + + and Co + + cations.
Claim 7
Dependent Claim
35 words
The improvement of claim 5, in which the solution of glucose contains about 2 to about 200 ppm of Mg + + cations and about 1 to about 100 ppm of Co + + cations.
Claim 8
Dependent Claim
22 words
The improvement of claim 1, in which the isomerisation is carried out at a temperature of about 50° to about 75° C.
References Cited (Backward Citations)
US2676922A
Cited
DE1959169A1
Cited
US3715276A
Cited
US3708397A
Cited
US3788945A
Cited
US3834940A
Cited
US3868304A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-11-09 | 1980-05-10 | 1980-11-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-11-09 | 1984-05-10 | 1984-11-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-11-09 | 1988-05-10 | 1988-11-09 | $7700.00 | Window Closed / Lapsed |