Patent US4459361A successfully refreshed from Google Patents.
US4459361A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Ligand assay with one or two particulate reagents and filter
Assignee: Cambridge Biotech Corp
•
Filing: 1982-06-04
•
Granted: 1984-07-10
•
Expires: 2002-06-04
Abstract
Abstract Method of making and using products for analysis of specific ligands in solution. More specifically, a method of analyzing a sample for a ligand by incubating the sample with a ligand selective binder and with a predetermined amount of ligand, the binder and/or the predetermined amount of ligand being borne by particles of predetermined size, filtering the solution and analyzing the particles which pass through the filter and/or those particles that do not pass through the filter.
Inventors
- 👤 Malcolm L. Gefter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 23:34
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/824
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
107 words
A method of analyzing a sample for a ligand, comprising incubating the sample with a binder, the binder being a nonparticulate material which specifically binds to the ligand and which binder has a plurality of binding sites for the ligand, and with particles of predetermined size bearing a predetermined amount of ligand on their outer surface, passing the mixture through a filter having apertures which are larger than the ligand-bearing particles but smaller than aggregates of the ligand-bearing particles, and analyzing at least one of the group of (a) the particles which pass through the filter and (b) the particles which do not pass through the filter.
Claim 2
Independent Claim
107 words
A method of analyzing a sample for a ligand, comprising incubating the sample with a binder, the binder being a nonparticulate material which specifically binds to the ligand and which binder has a plurality of binding sites for the ligand, and with particles of predetermined size bearing a predetermined amount of ligand on their outer surface, passing the mixture through a filter having apertures which are larger than the ligand-bearing particles but smaller than aggregates of the ligand-bearing particles, and analyzing at least one of the group of (a) the particles which pass through the filter and (b) the particles which do not pass through the filter.
Claim 3
Dependent Claim
33 words
The method of claim 1, wherein the incubation is carried out for a sufficient length of time to reach equilibrium in the reaction between the binder, and the ligand and the ligand-bearing particles.
Claim 4
Dependent Claim
21 words
The method of claim 1, wherein the ligand and is a protein, and the binder is an antibody to that protein.
Claim 5
Dependent Claim
15 words
The method of claim 1, wherein the particles which pass through the filter are analyzed.
Claim 6
Dependent Claim
17 words
The method of claim 4, wherein the analysis comprises determining the concentration of particles in the filtrate.
Claim 7
Dependent Claim
32 words
The method of claim 5, wherein the analysis of the filtrate is by comparison of the appearance of the filtrate with a visual standard corresponding to a known concentration of the ligand.
Claim 8
Dependent Claim
35 words
The method of claim 5, wherein the analysis of the filtrate is by meansuring the amount of polychromatic light or light of a predetermined wavelength which will pass through a standard volume of the filtrate.
Claim 9
Dependent Claim
27 words
The method of claim 4, wherein the particles are latex beads which are substantially uniform in size, having a diameter of from about 0.01 to 10 μm.
Claim 10
Dependent Claim
43 words
The method of claim 1, wherein the particles which do not pass through the filter are analyzed by resuspending the particles in liquid and determining the number of those particles relative to the total number of particles with which the sample was incubated.
Claim 11
Independent Claim
149 words
A method of analyzing a sample for a ligand, comprising incubating the sample with binder particles, which binder particles comprise particles of predetermined size having a material on their outer surface which specifically bind to the ligand, and with ligand-bearing particales of predetermined size bearing a predetermined amount of the ligand on their outer surface, passing the mixture through a filter which has apertures which are large enough to pass individual particles but smaller than aggregates of the binder particles and the ligand-bearing particles, either the ligand-bearing particles or the binder particles (1) being larger than the apertures and larger than the other particles, (2) bearing a small number of binding sites, and (3) being in excess of the other particles, and analyzing at least one of the group of (a) the particles which pass through the filter and (b) the particles which do not pass through the filter.
Claim 12
Independent Claim
149 words
A method of analyzing a sample for a ligand, comprising incubating the sample with binder particles, which binder particles comprise particles of predetermined size having a material on their outer surface which specifically bind to the ligand, and with ligand-bearing particales of predetermined size bearing a predetermined amount of the ligand on their outer surface, passing the mixture through a filter which has apertures which are large enough to pass individual particles but smaller than aggregates of the binder particles and the ligand-bearing particles, either the ligand-bearing particles or the binder particles (1) being larger than the apertures and larger than the other particles, (2) bearing a small number of binding sites, and (3) being in excess of the other particles, and analyzing at least one of the group of (a) the particles which pass through the filter and (b) the particles which do not pass through the filter.
Claim 13
Dependent Claim
33 words
The method of claim 10, wherein the incubation is carried out for a sufficient length of time to reach equilibrium in the reaction between the binder particles, the ligand and the ligand-bearing particles.
Claim 14
Dependent Claim
15 words
The method of claim 10, wherein the particles that pass through the filter are analyzed.
Claim 15
Dependent Claim
15 words
The method of claim 12, wherein the binder particles are larger than the filter apertures.
Claim 16
Dependent Claim
15 words
The method of claim 12, wherein the ligand-bearing particles are larger than the filter aperture.
Claim 17
Dependent Claim
111 words
An assay kit for analysis of a ligand, comprising binder particles which comprise particles of predetermined size bearing on their outside surface a binder material which specifically binds to the ligand, competitive binding particles comprising particles of a predetermined size bearing a predetermined amount of ligand on their outer surface, and a filter having apertures which are larger than the competitive binding particles but smaller than aggregates of competitive binder particles with binder particles, either the ligand-bearing particles or the binder particles (1) being larger than the apertures and larger than the other particles, (2) bearing a small number of binding sites, and (3) being in excess of the other particles.
Claim 18
Dependent Claim
111 words
An assay kit for analysis of a ligand, comprising binder particles which comprise particles of predetermined size bearing on their outside surface a binder material which specifically binds to the ligand, competitive binding particles comprising particles of a predetermined size bearing a predetermined amount of ligand on their outer surface, and a filter having apertures which are larger than the competitive binding particles but smaller than aggregates of competitive binder particles with binder particles, either the ligand-bearing particles or the binder particles (1) being larger than the apertures and larger than the other particles, (2) bearing a small number of binding sites, and (3) being in excess of the other particles.
Claim 19
Dependent Claim
25 words
The method of claim 9, wherein the particles which do not pass through the filter are disaggregated prior to determination of the number of particles.
Claim 20
Dependent Claim
44 words
The method of claim 10, wherein the amounts of binder and ligand borne by the particles, and the number of particles are such that, in the absence of inhibition, each of the binder particles binds with one or more of the ligand bearing particles.
Claim 21
Independent Claim
105 words
A method of analyzing a sample for a ligand, comprising incubating the sample with a binder and with a predetermined amount of ligand, the binder being a non-particulate material which specifically binds to the ligand at a plurality of binding sites, the binder being borne by particles of predetermined size on their outer surface, passing the mixture through a filter having apertures which are larger than the binder-bearing particles but smaller than aggregation of the binder-bearing particles, and analyzing at least one of the group of (a) the particles which pass through the filter and (b) the particles which do not pass through the filter.
Claim 22
Independent Claim
105 words
A method of analyzing a sample for a ligand, comprising incubating the sample with a binder and with a predetermined amount of ligand, the binder being a non-particulate material which specifically binds to the ligand at a plurality of binding sites, the binder being borne by particles of predetermined size on their outer surface, passing the mixture through a filter having apertures which are larger than the binder-bearing particles but smaller than aggregation of the binder-bearing particles, and analyzing at least one of the group of (a) the particles which pass through the filter and (b) the particles which do not pass through the filter.
Claim 23
Dependent Claim
30 words
The method of claim 18, wherein the incubation is carried out for a sufficient length of time to reach equilibrium in the reaction between the ligand and the binder-bearing particles.
Claim 24
Dependent Claim
20 words
The method of claim 18, wherein the ligand is a protein, and the binder is an antibody to that protein.
Claim 25
Dependent Claim
17 words
The method of claim 18, wherein the analysis comprises determining the concentration of particles in the filtrate.
Claim 26
Dependent Claim
32 words
The method of claim 21, wherein the analysis of the filtrate is by comparison of the appearance of the filtrate with a visual standard corresponding to a known concentration of the ligand.
Claim 27
Dependent Claim
43 words
The method of claim 18, wherein the particles which do not pass through the filter are analyzed by resuspending the particles in liquid and determining the number of those particles relative to the total number of particles with which the sample was incubated.
References Cited (Backward Citations)
US29474A
Cited
US3442819A
Cited
USRE29474E
Cited
US3492396A
Cited
US3862303A
Cited
US4138213A
Cited
US4143124A
Cited
US4062935A
Cited
US4091089A
Cited
US4202872A
Cited
US4308145A
Cited
US4210723A
Cited
US4308026A
Cited
US4115535A
Cited
US4235960A
Cited
US4191739A
Cited
US4184849A
Cited
US4256724A
Cited
US4279617A
Cited
US4305925A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-07-10 | 1988-01-09 | 1988-07-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-07-10 | 1992-01-09 | 1992-07-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-07-10 | 1996-01-09 | 1996-07-10 | $7700.00 | Window Closed / Lapsed |