US4210723A
Expired / Lapsed
US
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Method of coupling a protein to an epoxylated latex
Assignee: Dow Chemical Co
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Filing: 1977-11-25
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Granted: 1980-07-01
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Expires: 1997-11-25
Abstract
Abstract Proteins are coupled to polymer particles containing free epoxy groups in a latex. The polymer particles are about 0.15 to 1.5 micrometers in diameter, and have an inner core and an outer shell which contains the free epoxy groups. The coupled proteins are particularly useful in carrying out immunological tests.
Inventors
- 👤 Linneaus C. Dorman
- 👤 Inder Mani
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:53
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/76; C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/811; Y10S436/814; Y10S436/815; Y10S436/817; Y10S436/818
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
179 words
A method for coupling a protein having a reactive group containing a labile hydrogen to polymer particles containing free epoxy groups in a latex wherein the polymer particles in the latex range in size from about 0.15 to 1.5 micrometers in diameter and have an inner core and an outer shell containing free epoxy groups, said inner core being formed by the polymerization or copolymerization of one or more hard monomers and optionally soft monomer to produce a latex containing polymer particles that are said inner core and said outer shell being formed by copolymerization in the presence of said inner core polymer particles one or more hard monomers and a copolymerizable ethylenically unsaturated compound having free epoxy groups and optionally soft monomer, said method comprising reacting said polymer particles containing free epoxy groups in the latex and said protein at a pH of 7.0-9.0 for a time sufficient to form a covalent bond between the reactive group of the protein and the free epoxy group of the polymer particles and removing the unreacted protein from the latex-protein conjugate.
Claim 2
Independent Claim
179 words
A method for coupling a protein having a reactive group containing a labile hydrogen to polymer particles containing free epoxy groups in a latex wherein the polymer particles in the latex range in size from about 0.15 to 1.5 micrometers in diameter and have an inner core and an outer shell containing free epoxy groups, said inner core being formed by the polymerization or copolymerization of one or more hard monomers and optionally soft monomer to produce a latex containing polymer particles that are said inner core and said outer shell being formed by copolymerization in the presence of said inner core polymer particles one or more hard monomers and a copolymerizable ethylenically unsaturated compound having free epoxy groups and optionally soft monomer, said method comprising reacting said polymer particles containing free epoxy groups in the latex and said protein at a pH of 7.0-9.0 for a time sufficient to form a covalent bond between the reactive group of the protein and the free epoxy group of the polymer particles and removing the unreacted protein from the latex-protein conjugate.
Claim 3
Dependent Claim
29 words
The method of claim 1 wherein the latex containing the polymer particles having free epoxy groups is a monodispersed latex and the reactive group on the protein is amino.
Claim 4
Dependent Claim
21 words
The method of claim 1 wherein the outer shell on the polymer particles present in the latex is styrene-glycidyl methacrylate copolymer.
Claim 5
Dependent Claim
12 words
The method of claim 3 wherein the protein is human chorionic ganadotropin.
Claim 6
Dependent Claim
10 words
The method of claim 3 wherein the protein is insulin.
Claim 7
Dependent Claim
11 words
The method of claim 3 wherein the protein is an enzyme.
Claim 8
Dependent Claim
59 words
The method of claim 4 wherein the coupling site density of the styrene-glycidyl methacrylate copolymer outer shell is about one epoxy unit per 5 to 40 square angstrom units of particle surface area and the amount of human chorionic gonadotropin used to prepare the polymer particle protein conjugate is 1,000 to 25,000 international units per gram of polymer particles.
Claim 9
Dependent Claim
15 words
A protein polymer particle conjugate in a latex formed by the method of claim 2.
Claim 10
Dependent Claim
13 words
The protein-latex conjugate of claim 8 wherein the protein is human chorionic gonadotropin.
Claim 11
Dependent Claim
29 words
The protein-polymer particle conjugate in a latex of claim 8 wherein the protein is insulin and the latex contains polymer particles having an outer shell of styrene-glycidyl methacrylate copolymer.
Claim 12
Dependent Claim
16 words
The protein polymer particle conjugate in a latex of claim 8 wherein the latex is dyed.
Claim 13
Dependent Claim
32 words
A kit for immunologically testing for pregnancy which comprises as a first reagent the protein-polymer particle conjugate in a latex of claim 9 and as a second reagent, anti-human chorionic gonadotropin serum.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-07-01 | 1983-12-31 | 1984-07-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-07-01 | 1987-12-31 | 1988-07-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-07-01 | 1991-12-31 | 1992-07-01 | $7700.00 | Window Closed / Lapsed |