Patent DE3218314C2 successfully refreshed from Google Patents.
US3844892A
Expired / Lapsed
US
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Method of immobilizing enzymes
Assignee: Gulf Research and Development Co
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Filing: 1972-12-19
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Granted: 1974-10-29
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Expires: 1992-12-19
Abstract
Abstract Enzymes are immobilized by reaction with an epoxy containing polymer which is made by polymerizing an epoxy monomer containing a 1,2-epoxy group and terminal unsaturation with an olefin monomer such as acrylonitrile by free radical polymerization to form the epoxy containing polymer.
Inventors
- 👤 J Matthews
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 21:10
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/089; C12N11/082; C12N11/087
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
78 words
A METHOD FOR THE PREPARATION OF AN IMMOBILIZED ENZYME WHICH COMPRISES REACTING AN AQUEOUS SOLUTION OF AN ENZYME WITH AN EPOXY CONTAINING COPOLYMER PREPARED BY THE FREE RADICAL COPOLYMERIZATION OF AN EPOXY MONOMER HAVING AT LEAST ONE 1, 2-EPOXY GROUP AND ONE TERMINAL UNSATURATION WITH AN OLEFIN MONOMER HAVING THE FORMULA CH2=C(R1)-R4 IN WHICH R1 IS HYDROGEN OR METHYL AND R4 IS CYANO OR LOWER CARBOALKOXY WHEREBY SAID ENZYME IS CHEMICALLY BONDED THROUGH EPOXY GROUPS TO SAID COPOLYMER.
Claim 2
Independent Claim
78 words
A METHOD FOR THE PREPARATION OF AN IMMOBILIZED ENZYME WHICH COMPRISES REACTING AN AQUEOUS SOLUTION OF AN ENZYME WITH AN EPOXY CONTAINING COPOLYMER PREPARED BY THE FREE RADICAL COPOLYMERIZATION OF AN EPOXY MONOMER HAVING AT LEAST ONE 1, 2-EPOXY GROUP AND ONE TERMINAL UNSATURATION WITH AN OLEFIN MONOMER HAVING THE FORMULA CH2=C(R1)-R4 IN WHICH R1 IS HYDROGEN OR METHYL AND R4 IS CYANO OR LOWER CARBOALKOXY WHEREBY SAID ENZYME IS CHEMICALLY BONDED THROUGH EPOXY GROUPS TO SAID COPOLYMER.
Claim 3
Dependent Claim
36 words
A method for the preparation of an immobilized enzyme in accordance with claim 1 in which the epoxy monomer is selected from the group consisting of glycidyl acrylate, glycidyl methacrylate, allyl glycidyl ether, or mixtures thereof.
Claim 4
Dependent Claim
106 words
A method for the preparation of an immobilized enzyme in accordance with claim 1 in which the epoxy monomer is prepared by the reaction of a polyepoxide having an epoxy equivalent value greater than one with a difunctional olefin having an unsaturated group and a second functional group and defined by the structural formula CH2 C(R1)-R2 in which R1 is hydrogen or methyl and R2 is carboxyl; hydroxymethyl; formyl; chlorocarbonyl; carbamyl; aminomethyl; mercaptocarbonyl; mercaptomethyl; -CH2NHR3 where R3 is lower alkyl, hydroxyphenyl or lower alkyl substituted hydroxyphenyl; or lower alkyl or phenyl substituted hydroxyphenyl; said polyepoxide reacting with the difunctional olefin through the said second functional group.
Claim 5
Dependent Claim
40 words
A method for the preparation of an immobilized enzyme in accordance with claim 1 in which the epoxy monomer is between about 5 and about 30 mol percent of the comonomer mixture comprising the epoxy monomer and the olefin monomer.
Claim 6
Dependent Claim
23 words
A method for the preparation of an immobilized enzyme in accordance with claim 1 in which the olefin monomer is acrylonitrile or methacrylonitrile.
Claim 7
Dependent Claim
25 words
A method for the preparation of an immobilized enzyme in accordance with claim 3 in which the polyepoxide is a diglycidyl ether of bisphenol A.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-10-28 | 1978-04-29 | 1978-10-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-10-28 | 1982-04-29 | 1982-10-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-10-28 | 1986-04-29 | 1986-10-29 | $7700.00 | Window Closed / Lapsed |