US3853708A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Coupling biologically active substances to oxirane-containing polymers
Assignee: Exploaterings TBF AB
•
Filing: 1974-01-14
•
Granted: 1974-12-10
•
Expires: 1994-01-14
Abstract
Abstract Biologically active substances such as an enzyme or enzyme inhibitor are coupled to polymers in which epoxide groups have been introduced by reacting the polymer with a epihalohydrin or a bisoxirane.
Inventors
- 👤 C Eklund
- 👤 J Porath
- 👤 R Axen
- 👤 N Fornstedt
- 👤 L Sundberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 02:21
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/10; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/56; A61K47/58; A61K47/61; C07; C07K; C07K14/00; C07K14/81; C07K14/8107; C07K14/811; C08; C08F; C08F8/00
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
140 words
A METHOD FOR COUPLING WITH COVALENT BONDS A WATERINSOLUBLE POLYSACCHARIDE GEL AND AN ENZYME OR A PROTEINACEOUS ENZYME INHIBITOR, COMPRISING THE STEPS OF INTRODUCING AN EPONIDE GROUP-CONTAINING BIFUNCTIONAL COMPOUND SELECTED FROM THE CLASS CONSISTING OF EPIHALOHYDRIN AND BISOXIRANE IN THE INSOLUBLE POLYSACCHARIDE GEL IN AN ALKALINE MEDIUM BY REACTING SAID COMPOUND WITH HYDROXY GROUPS OF SAID POLYSACCHRIDE GEL, SEPARATING EXCESS SAID BIFUNCTIONAL COMPOUND FROM SAID INSOLUBLE POLYSACCHARIDE GEL, AND CONTACTING THE LATTER WITH SAID ENZYME OF ENZYME INHIBITOR TO CHEMICALLY COUPLE SAID ENZYME OR INZYME INHIBITOR TO SAID POLYSACCHARIDE GEL BY REACTING A FUNCTIONAL GROUP OF ENZYME OF ENZYME INHIBITOR WITH EPOSIDE GROUPS OF SAID POLYSACCHARIDE GEL WHEREBY SAID ENZYME OR ONZYME INHIBITOR RETAINS ITS ABILITY TO FORM COMPLEXES WITH NATURAL SUBSTANCES HAVING AFFINITY FOR SAID ENZYME OF ENZYME INHIBITOR IN ITS FREE FROM AND RETAINS ITS ACTIVITY ALMOST COMPLETELY.
Claim 2
Independent Claim
140 words
A METHOD FOR COUPLING WITH COVALENT BONDS A WATERINSOLUBLE POLYSACCHARIDE GEL AND AN ENZYME OR A PROTEINACEOUS ENZYME INHIBITOR, COMPRISING THE STEPS OF INTRODUCING AN EPONIDE GROUP-CONTAINING BIFUNCTIONAL COMPOUND SELECTED FROM THE CLASS CONSISTING OF EPIHALOHYDRIN AND BISOXIRANE IN THE INSOLUBLE POLYSACCHARIDE GEL IN AN ALKALINE MEDIUM BY REACTING SAID COMPOUND WITH HYDROXY GROUPS OF SAID POLYSACCHRIDE GEL, SEPARATING EXCESS SAID BIFUNCTIONAL COMPOUND FROM SAID INSOLUBLE POLYSACCHARIDE GEL, AND CONTACTING THE LATTER WITH SAID ENZYME OF ENZYME INHIBITOR TO CHEMICALLY COUPLE SAID ENZYME OR INZYME INHIBITOR TO SAID POLYSACCHARIDE GEL BY REACTING A FUNCTIONAL GROUP OF ENZYME OF ENZYME INHIBITOR WITH EPOSIDE GROUPS OF SAID POLYSACCHARIDE GEL WHEREBY SAID ENZYME OR ONZYME INHIBITOR RETAINS ITS ABILITY TO FORM COMPLEXES WITH NATURAL SUBSTANCES HAVING AFFINITY FOR SAID ENZYME OF ENZYME INHIBITOR IN ITS FREE FROM AND RETAINS ITS ACTIVITY ALMOST COMPLETELY.
Claim 3
Dependent Claim
12 words
A method according to claim 1, in which the polysaccharide is agar.
Claim 4
Dependent Claim
12 words
A method according to claim 1, in which the polysaccharide is agarose.
Claim 5
Dependent Claim
12 words
A method according to claim 1, in which the polysaccharide is cellulose.
Claim 6
Dependent Claim
23 words
A method according to claim 1, in which the polysaccharide is a member selected from the class consisting of dextran and cross-linked dextran.
Claim 7
Dependent Claim
12 words
A method according to claim 1, in which said compound is epichlorohydrin.
Claim 8
Dependent Claim
12 words
A method according to claim 1, in which said compound is epibromohydrin.
Claim 9
Dependent Claim
13 words
A method according to claim 1, in which said compound has the formula
Claim 10
Dependent Claim
24 words
A method according to claim 1, in which the coupling is performed in an aqueous medium whose water content is at least 70 percent.
References Cited (Backward Citations)
US3282702A
Cited
US3645852A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-09 | 1978-06-10 | 1978-12-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-09 | 1982-06-10 | 1982-12-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-09 | 1986-06-10 | 1986-12-10 | $7700.00 | Window Closed / Lapsed |