US4038140A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for binding biologically active proteins
Assignee: Roche Diagnostics GmbH
•
Filing: 1975-05-30
•
Granted: 1977-07-26
•
Expires: 1995-05-30
Abstract
Abstract Biologically active proteins are bound onto an insoluble carrier by reacting such protein in an aqueous phase with a carrier comprising an activated polysaccharide having a hydrophilic graft co-polmyer grafted thereinto.
Inventors
- 👤 Dieter Jaworek
- 👤 Joseph Maier
- 👤 Michael Nelbock-Hochstetter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:49
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/10; C08; C08F; C08F251/00; C08F8/00; C08F8/30
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
51 words
Process for binding a biologically active protein to an insoluble carrier which process comprises reacting at least one such protein in aqueous phase with a carrier comprising an activated polysaccharide with a hydrophilic graft co-polymer grafted thereinto, wherein said polysaccharide is activated by alkylation or acylation monomers, or by radical formation.
Claim 2
Dependent Claim
51 words
Process for binding a biologically active protein to an insoluble carrier which process comprises reacting at least one such protein in aqueous phase with a carrier comprising an activated polysaccharide with a hydrophilic graft co-polymer grafted thereinto, wherein said polysaccharide is activated by alkylation or acylation monomers, or by radical formation.
Claim 3
Dependent Claim
32 words
Process claimed in claim 1 wherein the graft co-polymerization of the hydrophilic monomer on the activated polysaccharide takes place in the presence of the protein upon simultaneous binding thereof to the carrier.
Claim 4
Dependent Claim
19 words
Process claimed in claim 2 wherein the protein, activated polysaccharide and hydrophilic graft co-monomer are contacted in aqueous phase.
Claim 5
Dependent Claim
47 words
Process claimed in claim 2 wherein the protein is first reacted with a linking compound containing a radical capable of acylating or alkylating protein in aqueous solution and a radical capable of copolymerization, and the resulting reaction product is contacted with said activated polysaccharide and graft co-monomer.
Claim 6
Dependent Claim
25 words
Process as claimed in claim 4 wherein a cross-linking agent is also added to the mixture of said reaction product, activated polysaccharide, and graft co-monomer.
Claim 7
Dependent Claim
25 words
Process as claimed in claim 1 wherein the hydrophilic graft co-monomer contains a minor portion of a monomer capable of direct bonding to a protein.
Claim 8
Dependent Claim
21 words
Process as claimed in claim 6 wherein the monomer capable of direct bonding to a protein is a dicarboxylic acid anhydride.
Claim 9
Dependent Claim
52 words
Process for binding a biologically active protein to an insoluble carrier which process comprises reacting at least one such protein in aqueous phase with a carrier comprising a polysaccharide with a hydrophilic graft co-polymer grafted thereinto, and subsequently activating said polysaccharide graft co-polymer by introducing a group capable of protein binding thereinto.
Claim 10
Dependent Claim
52 words
Process for binding a biologically active protein to an insoluble carrier which process comprises reacting at least one such protein in aqueous phase with a carrier comprising a polysaccharide with a hydrophilic graft co-polymer grafted thereinto, and subsequently activating said polysaccharide graft co-polymer by introducing a group capable of protein binding thereinto.
Claim 11
Dependent Claim
33 words
Process as claimed in claim 1 wherein said activated polysaccharide is selected from the group consisting of polysaccharide allyl ether, polysaccharide acrylic acid ester, polysaccharide methacrylic acid ester and polysaccharide maleic acid hemiester.
Claim 12
Dependent Claim
23 words
Process as claimed in claim 1 wherein the polysaccharide is selected from the group consisting of starch, cellulose derivatives, dextran derivatives and polyglucosides.
Claim 13
Dependent Claim
23 words
Process as claimed in claim 1 wherein the polysaccharide graft copolymer contains as the hydrophilic graft co-monomer a monomer consisting essentially of acrylamide.
Claim 14
Dependent Claim
14 words
Process as claimed in claim 1 wherein the biologically active protein is an enzyme.
Claim 15
Dependent Claim
15 words
Process as claimed in claim 1 wherein the biologically active protein is an enzyme association.
Claim 16
Dependent Claim
20 words
Process as claimed in claim 1 wherein the biologically active protein is a protein hormone, peptide hormone, antigen or anti-body.
Claim 17
Dependent Claim
12 words
Biologically active protein composition prepared by the process claimed in claim 1.
References Cited (Backward Citations)
US3764477A
Cited
US3625827A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-07-25 | 1981-01-24 | 1981-07-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-07-25 | 1985-01-24 | 1985-07-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-07-25 | 1989-01-24 | 1989-07-26 | $7700.00 | Window Closed / Lapsed |