US4091089A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for quantitative determination of an antigenic substance
Assignee: Chugai Pharmaceutical Co Ltd
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Filing: 1976-03-31
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Granted: 1978-05-23
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Expires: 1996-03-31
Abstract
Abstract A method for the quantitative determination of antigenic substance without any confirmation test which comprises adding to a sample to be assayed an exogenous antigenic substance in a certain amount, combining the resulting mixture with an antibody specific to the antigenic substance to cause antigen-antibody reaction and measuring the amount of the precipitates formed, and a diagnostic kit for practicing the method are disclosed.
Inventors
- 👤 Kenji Chichibu
- 👤 Kiyoshige Wakabayashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 17:53
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/75; G01N21/77; G01N21/82; G01N33/00; G01N33/48; G01N33/50; G01N33/53; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/807; Y10S436/808; Y10S436/815
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
99 words
A method for the quantitative determination of antigenic substance which comprises adding to a sample the same exogenous antigenic substance as that to be assayed, separately prepared, in such an amount as to give the maximum precipitation by the reaction with the antibody used, combining the resulting mixtures with an antibody specific to the antigenic substance to be assayed to cause antigen-antibody reaction, and measuring turbidimetrically the amount of precipitates formed by the reaction, whereby the amount of precipitate will always be on the antigen-surplus side of the precipitate versus antigen curve formed for any given amount of antibody.
Claim 2
Independent Claim
99 words
A method for the quantitative determination of antigenic substance which comprises adding to a sample the same exogenous antigenic substance as that to be assayed, separately prepared, in such an amount as to give the maximum precipitation by the reaction with the antibody used, combining the resulting mixtures with an antibody specific to the antigenic substance to be assayed to cause antigen-antibody reaction, and measuring turbidimetrically the amount of precipitates formed by the reaction, whereby the amount of precipitate will always be on the antigen-surplus side of the precipitate versus antigen curve formed for any given amount of antibody.
Claim 3
Dependent Claim
41 words
A method for the quantitative determination of an antigenic substance according to claim 1 in which the antigenic substance is selected from the group consisting of immunoglobulin G, immunoglobulin A, immunoglobulin M, albumin, α 1 -antitrypsin, transferrin, heptoglobin and complement components.
References Cited (Backward Citations)
US3284434A
Cited
US3901654A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-05-22 | 1981-11-21 | 1982-05-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-05-22 | 1985-11-21 | 1986-05-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-05-22 | 1989-11-21 | 1990-05-23 | $7700.00 | Window Closed / Lapsed |