US3901654A
Expired / Lapsed
US
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Receptor assays of biologically active compounds employing biologically specific receptors
Assignee: Biological Developments Inc
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Filing: 1971-06-21
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Granted: 1975-08-26
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Expires: 1991-06-21
Abstract
Abstract This invention relates to novel assays for biologically active substrates employing purified target receptors. The assays comprise fluorescent assays for quenching and enhancement as well as radiochemical assays, employing labeled receptor in one embodiment and labeled substrate in another. Methods for the preparation of these purified receptors are also disclosed.
Inventors
- 👤 Stanley J Gross
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 07:11
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/542; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/20; Y10T436/200833; Y10T436/203332
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
100 words
AFLUORESCENT METHOD OF ASSAYING QUANTITIVELY FOR A COMPOUND WHICH IS SPECIFIC FOR A RECEPTOR COMPOUND, WHICH COMPRISES PRODUCING A MIXTURE, SUITABL FOR FLUOROMETRIC ASSAY, OF (1) A SAMPLE TO BE ANALYZED, (2) A KNOWN QUANTITY OF THE RECEPTOR COMPOUND IN ITS PURIFIED FORM AND WHICH IS BIOLOGICALLY SPECIFIC FOR THE COMPOUND TO BE ASSAYED, AND (3) A KNOWN QUANTITY OF A FLUORESCENCE QUENCHING COMPOUND, POSSESSING AN ABSORBANCE SPECTRUM CORRESPONDING TO THE EMISSION SPECTRUM OF THE RECEPTOR, AND WHICH IS HOMOLOGOUS TO SAID ASSAY COMPOUND SO AS TO BIND WITH SAID RECEPTOR COMPETITIVELY, AN ANALYZING SAID MIXTURE FOR RECEPTOR FLUORESCENCE QUENCHING.
Claim 2
Dependent Claim
100 words
AFLUORESCENT METHOD OF ASSAYING QUANTITIVELY FOR A COMPOUND WHICH IS SPECIFIC FOR A RECEPTOR COMPOUND, WHICH COMPRISES PRODUCING A MIXTURE, SUITABL FOR FLUOROMETRIC ASSAY, OF (1) A SAMPLE TO BE ANALYZED, (2) A KNOWN QUANTITY OF THE RECEPTOR COMPOUND IN ITS PURIFIED FORM AND WHICH IS BIOLOGICALLY SPECIFIC FOR THE COMPOUND TO BE ASSAYED, AND (3) A KNOWN QUANTITY OF A FLUORESCENCE QUENCHING COMPOUND, POSSESSING AN ABSORBANCE SPECTRUM CORRESPONDING TO THE EMISSION SPECTRUM OF THE RECEPTOR, AND WHICH IS HOMOLOGOUS TO SAID ASSAY COMPOUND SO AS TO BIND WITH SAID RECEPTOR COMPETITIVELY, AN ANALYZING SAID MIXTURE FOR RECEPTOR FLUORESCENCE QUENCHING.
Claim 3
Dependent Claim
49 words
The method of claim 1 wherein said fluorescence quenching compound is a fluorescent compound capable of being enhanced or polarized and which is homologous to said assay compound so as to bind with said receptor competitively; and analyzing said mixture for fluorescence enhancement or polarization of said fluorescent compound.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-08-25 | 1979-02-24 | 1979-08-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-08-25 | 1983-02-24 | 1983-08-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-08-25 | 1987-02-24 | 1987-08-26 | $7700.00 | Window Closed / Lapsed |