Patent US2183330A successfully refreshed from Google Patents.
US4138213A
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Agglutination immunoassay of immune complex with RF or Clq
Assignee: Bayer Corp
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Filing: 1977-05-20
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Granted: 1979-02-06
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Expires: 1997-05-20
Abstract
Abstract A method of determining Ab:Ag complex in a biological fluid sample comprising adding to the sample a solution of RF or Clq and a known quantity of a material, such as immunoglobulin-coated polystyrene particles, which is caused to agglutinate on contact with RF or Clq, and detecting the amount of material which is not agglutinated.
Inventors
- 👤 Pierre L. Masson
- 👤 Joseph Heremans, deceased
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:10
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54306; G01N33/564; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/821
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
52 words
A method of determining the presence in, or absence from, a biological fluid sample of an Ab:Ag complex, which comprises adding to the sample a solution of RF or Clq and a material which is caused to agglutinate on contact with RF or Clq, and detecting whether agglutination of the material occurs.
Claim 2
Independent Claim
52 words
A method of determining the presence in, or absence from, a biological fluid sample of an Ab:Ag complex, which comprises adding to the sample a solution of RF or Clq and a material which is caused to agglutinate on contact with RF or Clq, and detecting whether agglutination of the material occurs.
Claim 3
Independent Claim
54 words
A method of quantitatively determining the amount of Ab:Ag complex present in a biological fluid sample comprising adding to the sample a solution of RF or Clq and a known quantity of material which is caused to agglutinate on contact with RF or Clq, and detecting the amount of material which is not agglutinated.
Claim 4
Independent Claim
54 words
A method of quantitatively determining the amount of Ab:Ag complex present in a biological fluid sample comprising adding to the sample a solution of RF or Clq and a known quantity of material which is caused to agglutinate on contact with RF or Clq, and detecting the amount of material which is not agglutinated.
Claim 5
Dependent Claim
16 words
The method of claim 2, wherein the material comprises an immunoglobulin coating on inert carrier particles.
Claim 6
Dependent Claim
10 words
The method of claim 3, wherein the particles comprise polystyrene.
Claim 7
Dependent Claim
19 words
The method of claim 2, wherein the amount of material which is not agglutinated is determined by particle counting.
Claim 8
Independent Claim
114 words
A method of analyzing an Ab or Ag in a biological fluid sample comprising the steps of: a. adding to the sample an excess of an Ag or Ab which is specific to the Ab or Ag, respectively, in the sample to form an Ab:Ag complex; b. adding to the mixture formed in step (a) a known amount of a solution of RF or Clq in excess of that required to bind with all the said Ab:Ag complex; c. adding to the mixture formed in step (b) a known quantity of a material which is caused to agglutinate on contact with RF or Clq; d. determining the amount of material which is not agglutinated.
Claim 9
Independent Claim
114 words
A method of analyzing an Ab or Ag in a biological fluid sample comprising the steps of: a. adding to the sample an excess of an Ag or Ab which is specific to the Ab or Ag, respectively, in the sample to form an Ab:Ag complex; b. adding to the mixture formed in step (a) a known amount of a solution of RF or Clq in excess of that required to bind with all the said Ab:Ag complex; c. adding to the mixture formed in step (b) a known quantity of a material which is caused to agglutinate on contact with RF or Clq; d. determining the amount of material which is not agglutinated.
Claim 10
Dependent Claim
16 words
The method of claim 6, wherein the material comprises an immunoglobulin coating on inert carrier particles.
Claim 11
Dependent Claim
10 words
The method of claim 7, wherein the particles comprise polystyrene.
Claim 12
Dependent Claim
19 words
The method of claim 6, wherein the amount of material which is not agglutinated is determined by particle counting.
Claim 13
Dependent Claim
171 words
An automated method for determining the amount of an Ab or Ag in a biological fluid sample comprising the steps of: a. introducing the sample into an automated particle counting apparatus and adding to the sample an excess of an Ag or Ab which is specific to the Ab or Ag, respectively, in the sample to form an Ab:Ag complex; b. adding to the mixture formed in step (a) a known amount of a solution of RF or Clq in excess of that required to bind with all the said Ab:Ag complex; c. incubating the mixture formed in step (b) in a heating zone; d. removing the incubated mixture formed in step (c) from the heating zone and adding to the incubated mixture a known quantity of a material which is caused to agglutinate on contact with RF or Clq; e. incubating the mixture formed in step (d) in a heating zone; f. counting the number of unagglutinated particles of the material remaining in the incubated mixture formed in step (e).
Claim 14
Dependent Claim
171 words
An automated method for determining the amount of an Ab or Ag in a biological fluid sample comprising the steps of: a. introducing the sample into an automated particle counting apparatus and adding to the sample an excess of an Ag or Ab which is specific to the Ab or Ag, respectively, in the sample to form an Ab:Ag complex; b. adding to the mixture formed in step (a) a known amount of a solution of RF or Clq in excess of that required to bind with all the said Ab:Ag complex; c. incubating the mixture formed in step (b) in a heating zone; d. removing the incubated mixture formed in step (c) from the heating zone and adding to the incubated mixture a known quantity of a material which is caused to agglutinate on contact with RF or Clq; e. incubating the mixture formed in step (d) in a heating zone; f. counting the number of unagglutinated particles of the material remaining in the incubated mixture formed in step (e).
Claim 15
Dependent Claim
16 words
The method of claim 10, wherein the material comprises an immunoglobulin coating on inert carrier particles.
Claim 16
Dependent Claim
10 words
The method of claim 11, wherein the particles comprise polystyrene.
References Cited (Backward Citations)
US3658982A
Cited
US3689632A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-02-05 | 1982-08-07 | 1983-02-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-02-05 | 1986-08-07 | 1987-02-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-02-05 | 1990-08-07 | 1991-02-06 | $7700.00 | Window Closed / Lapsed |