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US3689632A
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Rheumatoid agglutination test and reagent
Assignee: Kowa Co Ltd
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Filing: 1969-01-06
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Granted: 1972-09-05
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Expires: 1989-01-06
Abstract
Abstract A rheumatoid agglutination test reagent, which can be used for detecting rheumatoid factor in whole blood as well as serum, and which demonstrates excellent results with respect to its sensitivity, reproducibility in the detection, comprising a buffered saline of a pH 7.5 + OR - 1.5 wherein are contained proper amounts of gamma -globulin coated resin particles formed by combining an ion-exchange resin with human gamma -globulin in the said solution and one class of assistant selected from the group consisting of hemolytic agent and coloring agent which can color the said gamma -globulin, method for preparation thereof and a diagnostic method of using some.
Inventors
- 👤 Yutaka Mizushima
- 👤 Hidetaka Nagai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 23:04
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/564; G01N2800/00; G01N2800/10; G01N2800/101; G01N2800/102; Y; Y10; Y10S; Y10S436/00; Y10S436/805
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
23 words
The reagent of claim 1 wherein said hemolytic agent is selected from the group consisting of saponin, nonion polyoxyethylene lauryl ether and laurylamine.
Claim 2
Dependent Claim
32 words
The reagent of claim 1 wherein at least 75 percent by weight of said weakly basic ion-exchange resin particles have an average particle diameter of from about 0.5 to about 3 microns.
Claim 3
Dependent Claim
45 words
The reagent of claim 1 wherein said reagent further contains: d. 0.005 - 0.5 percent by weight of a preservative selected from the group consisting of sodium azide, cresol, chlorothymol, thymol, para-oxybenzoic acid esters, thimerosal, mercuric phenyl acetate, mercuric phenyl sulfate and mercuric phenyl borate.
Claim 4
Dependent Claim
32 words
The reagent of claim 1 wherein said reagent further contains: e. 0.005 - 1.0 percent by weight of a stabilizer selected from the group consisting of acetyl tryptophan, albumin, peptone and gelatin.
Claim 5
Independent Claim
77 words
A method of preparing a rheumatoid agglutination test reagent when comprises contacting weakly basic ion-exchange resin particles with human gamma -globulin in a buffered saline solution having a pH of from about 6 to about 9 so as to form weakly basic ion-exchange particles coated with said human gamma -globulin and subsequently adding thereto a hemolytic agent the resulting reagent comprising 0.05 - 2 percent by weight of each of said hemolytic agent and globulin coated particles.
Claim 6
Dependent Claim
23 words
The method of claim 6 wherein said hemolytic agent is selected from the group consisting of saponin, nonionic polyoxyethylene lauryl ether and laurylamine.
Claim 7
Dependent Claim
31 words
The method of claim 6 wherein at least 75 percent by weight of said weakly basic ion-exchange resin particles have an average diameter of from about 0.5 to about 3 microns.
Claim 8
Independent Claim
66 words
A method of diagnosing rheumatoid factor which comprises mixing human whole blood with an effective agglutinating amount of a test reagent which comprises a buffered saline solution of a pH of from about 6 to about 9 containing: a. 0.5 - 2 percent by weight of weakly basic ion-exchange particles coated with human gamma -globulin; and b. 0.05 - 2 percent by weight of hemolytic agent.
Claim 9
Dependent Claim
23 words
The method of claim 9 wherein said hemolytic agent is selected from the group consisting of saponin, nonionic polyoxyethylene lauryl ether and laurylamine.
Claim 10
Dependent Claim
32 words
The method of claim 9 wherein at least 75 percent by weight of said weakly basic ion-exchange resin particles have an average particle diameter of from about 0.5 to about 3 microns.
Claim 11
Dependent Claim
46 words
The method of claim 9 wherein said test reagent further contains: d. 0.005 - 0.5 percent by weight of a preservative selected from the group consisting of sodium azide, cresol, chlorothymol, thymol, para-oxybenzoic acid esters thimerosal, mercuric phenyl acetate, mercuric phenyl sulfate and mercuric phenyl borate.
Claim 12
Dependent Claim
33 words
The method of claim 9 wherein said test reagent further contains: e. 0.005 - 1.0 percent by weight of a stabilizer selected from the group consisting of acetyl tryptophan, albumin, peptone and gelatin.
References Cited (Backward Citations)
US3088875A
Cited
US3074853A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-09-05 | 1976-03-06 | 1976-09-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-09-05 | 1980-03-06 | 1980-09-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-09-05 | 1984-03-06 | 1984-09-05 | $7700.00 | Window Closed / Lapsed |