Patent US4372883A successfully refreshed from Google Patents.
US4372883A
Expired / Lapsed
US
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Process for the production of vaccine
Assignee: Hayashibara Seibutsu Kagaku Kenkyujo KK
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Filing: 1980-08-18
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Granted: 1983-02-08
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Expires: 2000-08-18
Abstract
Abstract The present invention relates to a process for the production of vaccine, characterized by covalent attachment of a biologically toxic substance to a saccharide both to form a biologically toxic substance-saccharide conjugate and to detoxify said substance, followed by collection of the formed conjugate. According to the invention, various vaccine products having a high productivity of immunoglobulin G and M antibodies, but without productivity of immunoglobulin E antibody, are easily obtainable. In addition, the vaccine products, upon administration to animals, do not effect any allergic reaction and/or anaphylactic-shock due to the lack of the immunoglobulin E antibody production.
Inventors
- 👤 Tyoku Matuhashi
- 👤 Mitsuko Usui
- 👤 Akio Yamamoto
- 👤 Masakazu Mitsuhashi
- 👤 Shunsaku Koyama
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 16:24
CPC Classifications
A; A61; A61K; A61K39/00; A61K47/00; A61K47/50; A61K47/51; A61K47/56; A61K47/61; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S530/00; Y10S530/806; Y10S530/82; Y10S530/825; Y10S530/826; Y10S530/855; Y10S530/856
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
65 words
A process for the production of vaccine, consisting of the steps of: covalently attaching a biologically toxic substance to a saccharide selected from the group consisting of pullulan, elsinan and partial hydrolysates of pullulan or elsinan having an average molecular weight of 500 to 1,000,000, to form a biologically toxic substance-saccharide conjugate in which the biologically toxic substance is detoxified; and collecting the resultant conjugate.
Claim 2
Dependent Claim
65 words
A process for the production of vaccine, consisting of the steps of: covalently attaching a biologically toxic substance to a saccharide selected from the group consisting of pullulan, elsinan and partial hydrolysates of pullulan or elsinan having an average molecular weight of 500 to 1,000,000, to form a biologically toxic substance-saccharide conjugate in which the biologically toxic substance is detoxified; and collecting the resultant conjugate.
Claim 3
Dependent Claim
45 words
A process as set forth in claim 1, wherein said biologically toxic substance is one or more members selected from the group consisting of diphtheria toxin, tetanus toxin, botulinus toxin, gas gangrene toxin, cholera toxin, Agkistrodon halys venom, Trimeresurus flavoviridis venom, scorpion zootoxin, and ricin.
Claim 4
Dependent Claim
55 words
A process as set forth in claim 1, wherein said covalent attachment is effected with a saccharide and biologically toxic substance in the weight ratio of 1:100 to 100:1, at a pH in the range from 3 to 12, and a temperature in the range from 0° to 100° C. for 0.1 to 50 hours.
Claim 5
Dependent Claim
28 words
A process in accordance with claim 1, wherein said biologically toxic substance is one or more members selected from the group consisting of bacterial toxins, zootoxins and phytotoxins.
Claim 6
Dependent Claim
11 words
A vaccine produced in accordance with the process of claim 1.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-02-07 | 1986-08-09 | 1987-02-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-02-07 | 1990-08-09 | 1991-02-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-02-07 | 1994-08-09 | 1995-02-08 | $7700.00 | Window Closed / Lapsed |