US6171797B1
Expired / Lapsed
US
⏱️ 0.0 years remaining
Methods of making polymeric arrays
Assignee: Agilent Technologies Inc
•
Filing: 1999-10-20
•
Granted: 2001-01-09
•
Expires: 2019-10-20
Abstract
Abstract Methods are provided for making arrays of distinct polymers covalently bonded to the surface of the a solid support. In the subject methods, at least two distinct polymers, e.g. nucleic acids, are contacted with the surface of a solid support under conditions sufficient for the nucleic acids to become covalently bonded to the surface of the solid support through a cycloaddition reaction, e.g. through the reaction of a diene with a dienophile. Also provided are arrays produced by the subject methods, kits comprising the same and methods for using the arrays in analyte detection, e.g. hybridization, assays.
Inventors
- 👤 Michel G. M. Perbost
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 18:17
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353; C; C07; C07H; C07H21/00; B; B01; B01J; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00497; B01J2219/00527; B01J2219/00583; B01J2219/00585; B01J2219/0059; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/00612; B01J2219/00623; B01J2219/00626; B01J2219/00632; B01J2219/00637; B01J2219/00659; B01J2219/00718; B01J2219/0072; B01J2219/00722; C07B; C07B2200/00; C07B2200/11; C40; C40B; C40B40/00; C40B40/04; C40B40/06
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
72 words
A method of making an array of at least two distinct polymeric compounds of differing composition covalently bonded to a surface of a solid support, said method comprising: contacting a surface of a solid support having a cycloaddition reactive group and a contact angle ranging from about 20° to 100° with said distinct polymeric compounds under conditions sufficient for said polymeric compounds to covalently bond to said surface by a cycloaddition reaction.
Claim 2
Independent Claim
72 words
A method of making an array of at least two distinct polymeric compounds of differing composition covalently bonded to a surface of a solid support, said method comprising: contacting a surface of a solid support having a cycloaddition reactive group and a contact angle ranging from about 20° to 100° with said distinct polymeric compounds under conditions sufficient for said polymeric compounds to covalently bond to said surface by a cycloaddition reaction.
Claim 3
Dependent Claim
20 words
The method according to claim 1 , wherein said cycloaddition reaction comprises the reaction of a diene with a dienophile.
Claim 4
Dependent Claim
22 words
The method according to claim 1 , wherein said cycloaddition reaction produces a six membered ring between said polymers and said surface.
Claim 5
Dependent Claim
13 words
The method according to claim 1 , wherein said polymers are nucleic acids.
Claim 6
Independent Claim
80 words
A method of producing an array of at least two distinct nucleic acids that differ by sequence covalently bonded to the surface of a solid support, said method comprising: contacting a surface of a solid support having a cycloaddition reactive group and a contact angle ranging from about 20 to 100° with said nucleic acids under conditions sufficient for said nucleic acids to covalently bond to said surface by a Diels-Alder reaction; whereby said array of nucleic acids is produced.
Claim 7
Independent Claim
80 words
A method of producing an array of at least two distinct nucleic acids that differ by sequence covalently bonded to the surface of a solid support, said method comprising: contacting a surface of a solid support having a cycloaddition reactive group and a contact angle ranging from about 20 to 100° with said nucleic acids under conditions sufficient for said nucleic acids to covalently bond to said surface by a Diels-Alder reaction; whereby said array of nucleic acids is produced.
Claim 8
Dependent Claim
22 words
The method according to claim 5 , wherein a terminus of said nucleic acids comprises diene and said surface comprises a dienophile.
Claim 9
Dependent Claim
23 words
The method according to claim 5 , wherein a terminus of said nucleic acids comprises a dienophile and said surface comprises a diene.
Claim 10
Dependent Claim
14 words
The method according to claim 5 , wherein said dienophile comprises a double bond.
Claim 11
Dependent Claim
14 words
The method according to claim 5 , wherein said nucleic acid is a polydeoxyribonucleotide.
Claim 12
Independent Claim
93 words
A method of producing an array of at least two distinct polydeoxyribonucleotides that differ by sequence covalently bonded to the surface of a solid support, said method comprising: contacting a surface of a solid support having a cycloaddition reactive group and a contact angle ranging from about 20 to 100° with said polydeoxyribonulcucleotides under conditions sufficient for said nucleic acids to covalently bond to said surface by a Diels-Alder reaction, wherein a terminus of said polydeoxyribonucleotides comprises a diene and said surface comprises a dienophile; whereby said array of nucleic acids is produced.
Claim 13
Independent Claim
93 words
A method of producing an array of at least two distinct polydeoxyribonucleotides that differ by sequence covalently bonded to the surface of a solid support, said method comprising: contacting a surface of a solid support having a cycloaddition reactive group and a contact angle ranging from about 20 to 100° with said polydeoxyribonulcucleotides under conditions sufficient for said nucleic acids to covalently bond to said surface by a Diels-Alder reaction, wherein a terminus of said polydeoxyribonucleotides comprises a diene and said surface comprises a dienophile; whereby said array of nucleic acids is produced.
Claim 14
Dependent Claim
21 words
The method according to claim 10 , wherein said contacting comprises depositing an aqueous composition of said polydeoxyribonucleotides onto said surface.
Claim 15
Dependent Claim
16 words
The method according to claim 11 , wherein said depositing is by a drop deposition device.
Claim 16
Dependent Claim
16 words
The method according to claim 11 , wherein said depositing is by an ink jet device.
Claim 17
Independent Claim
190 words
A method of making a polymeric array of at least: (a) a first spot having a diameter ranging from 10 to 1000 μm and made up of a first polymer covalently bound to a surface; and (b) a second spot having a diameter ranging from 10 to 1000 μm and made up of a second polymer covalently bound to said surface of said solid support; said method comprising: depositing: (a) from about 1 nl to 1 pl of a first fluid composition comprising said first polymer onto a surface having a cycloaddition reactive group and a contact angle ranging from about 20 to 100° to produce said first spot having a diameter ranging from 10 to 1000 μm; and (b) from about 1 nl to 1 pl of a second fluid comprising said second polymer onto said surface to produce said second spot having a diameter ranging from 10 to 1000 μm, wherein said first and second fluid compositions are deposited onto said surface under conditions sufficient for said first and second polymeric compounds to covalently bond to said surface by a cycloaddition reaction; whereby said polymeric array is produced.
Claim 18
Independent Claim
190 words
A method of making a polymeric array of at least: (a) a first spot having a diameter ranging from 10 to 1000 μm and made up of a first polymer covalently bound to a surface; and (b) a second spot having a diameter ranging from 10 to 1000 μm and made up of a second polymer covalently bound to said surface of said solid support; said method comprising: depositing: (a) from about 1 nl to 1 pl of a first fluid composition comprising said first polymer onto a surface having a cycloaddition reactive group and a contact angle ranging from about 20 to 100° to produce said first spot having a diameter ranging from 10 to 1000 μm; and (b) from about 1 nl to 1 pl of a second fluid comprising said second polymer onto said surface to produce said second spot having a diameter ranging from 10 to 1000 μm, wherein said first and second fluid compositions are deposited onto said surface under conditions sufficient for said first and second polymeric compounds to covalently bond to said surface by a cycloaddition reaction; whereby said polymeric array is produced.
Claim 19
Dependent Claim
19 words
The method according to claim 14 , wherein said cycloaddition reaction comprises the reaction of a diene with dienophile.
Claim 20
Dependent Claim
25 words
The method according to claim 14 , wherein said cycloaddition reaction produces a six membered ring between said first and second polymers and said surface.
Claim 21
Dependent Claim
13 words
The method according to claim 14 , wherein said polymers are nucleic acids.
Claim 22
Independent Claim
196 words
A method of making a nucleic acid array of at least: (a) a first spot having a diameter ranging from 10 to 1000 μm and made up of a first nucleic acid covalently bound to a surface; and (b) a second spot having a diameter ranging from 10 to 1000 μm and made up of a second nucleic acid covalently bound to said surface of said solid support; said method comprising: depositing: (a) from about 1 nl to 1 pl of a first fluid composition comprising said first nucleic acid onto a surface having a cycloaddition reactive group and a contact angle ranging from about 20 to 100° to produce said first spot having a diameter ranging from 10 to 1000 μm; and (b) from about 1 nl to 1 pl of a second fluid comprising said second nucleic acid onto said surface to produce said second spot having a diameter ranging from 10 to 1000 μm, wherein said first and second fluid compositions are deposited onto said surface under conditions sufficient for said first and second nucleic acids to covalently bond to said surface by Diels-Alder reaction: whereby said array of nucleic acids is produced.
Claim 23
Independent Claim
196 words
A method of making a nucleic acid array of at least: (a) a first spot having a diameter ranging from 10 to 1000 μm and made up of a first nucleic acid covalently bound to a surface; and (b) a second spot having a diameter ranging from 10 to 1000 μm and made up of a second nucleic acid covalently bound to said surface of said solid support; said method comprising: depositing: (a) from about 1 nl to 1 pl of a first fluid composition comprising said first nucleic acid onto a surface having a cycloaddition reactive group and a contact angle ranging from about 20 to 100° to produce said first spot having a diameter ranging from 10 to 1000 μm; and (b) from about 1 nl to 1 pl of a second fluid comprising said second nucleic acid onto said surface to produce said second spot having a diameter ranging from 10 to 1000 μm, wherein said first and second fluid compositions are deposited onto said surface under conditions sufficient for said first and second nucleic acids to covalently bond to said surface by Diels-Alder reaction: whereby said array of nucleic acids is produced.
Claim 24
Dependent Claim
27 words
The method according to claim 18 , wherein a terminus of each of said first and second nucleic acids comprises diene and said surface comprises a dienophile.
Claim 25
Dependent Claim
14 words
The method according to claim 19 , wherein said dienophile comprises a double bond.
Claim 26
Dependent Claim
25 words
The method according to claim 18 , wherein a terminus of each of said nucleic acids comprises a dienophile and said surface comprises a diene.
Claim 27
Dependent Claim
14 words
The method according to claim 21 , wherein said dienophile comprises a double bond.
Claim 28
Dependent Claim
13 words
The method according to claim 18 , wherein said nucleic acids are polydeoxyribonucleotides.
Claim 29
Independent Claim
190 words
A method of making a polydeoxyribonucleotide array of at least: (a) a first spot having a diameter ranging from 10 to 1000 μm and made up of a first polydeoxyribonucleotide covalently bound to a surface; and (b) a second spot having a diameter ranging from 10 to 1000 μm and made up of a second polydeoxyribonucleotide covalently bound to said surface of said solid support; said method comprising: depositing: (a) from about 1 nl to 1 pl of a first fluid composition comprising said first polydeoxyribonucleotide onto a surface having a cycloaddition reactive group and a contact angle ranging from about 20 to 100° to produce said first spot having a diameter ranging from 10 to 1000 μm; and (b) from about 1 nl to 1 pl of a second fluid comprising said second polydeoxyribonucleotide onto said surface to produce said second spot having a diameter ranging from 10 to 1000 μm, wherein said first and second fluid compositions are deposited onto said surface under conditions sufficient for said first and second polydeoxyribonucleotide to covalently bond to said surface by a Diels-Alder reaction; whereby said array of polydeoxyribonucleotides is produced.
Claim 30
Independent Claim
190 words
A method of making a polydeoxyribonucleotide array of at least: (a) a first spot having a diameter ranging from 10 to 1000 μm and made up of a first polydeoxyribonucleotide covalently bound to a surface; and (b) a second spot having a diameter ranging from 10 to 1000 μm and made up of a second polydeoxyribonucleotide covalently bound to said surface of said solid support; said method comprising: depositing: (a) from about 1 nl to 1 pl of a first fluid composition comprising said first polydeoxyribonucleotide onto a surface having a cycloaddition reactive group and a contact angle ranging from about 20 to 100° to produce said first spot having a diameter ranging from 10 to 1000 μm; and (b) from about 1 nl to 1 pl of a second fluid comprising said second polydeoxyribonucleotide onto said surface to produce said second spot having a diameter ranging from 10 to 1000 μm, wherein said first and second fluid compositions are deposited onto said surface under conditions sufficient for said first and second polydeoxyribonucleotide to covalently bond to said surface by a Diels-Alder reaction; whereby said array of polydeoxyribonucleotides is produced.
Claim 31
Dependent Claim
27 words
The method according to claim 24 , wherein a terminus of each of said first a second polydeoxyribonucleotides comprises a diene and said surface comprises a dienophile.
Claim 32
Dependent Claim
27 words
The method according to claim 24 , wherein a terminus of each of said first a second polydeoxyribonucleotides comprises a dienophile and said surface comprises a diene.
Claim 33
Dependent Claim
16 words
The method according to claim 24 , wherein said depositing is by a drop deposition device.
Claim 34
Dependent Claim
16 words
The method according to claim 24 , wherein said depositing is by an ink jet device.
Claim 35
Dependent Claim
16 words
The method according to claim 1 wherein the contact angle ranges from about 40 to 100°.
Claim 36
Dependent Claim
16 words
The method according to claim 10 wherein the contact angle ranges from about 40 to 100°.
Claim 37
Dependent Claim
16 words
The method according to claim 14 wherein the contact angle ranges from about 60 to 100°.
Claim 38
Dependent Claim
16 words
The method according to claim 24 wherein the contact angle ranges from about 40 to 100°.
References Cited (Backward Citations)
US5436327A
Cited
US5405783A
Cited
US5527681A
Cited
US5424186A
Cited
US5445934A
Cited
US5561071A
Cited
US5639603A
Cited
US5242974A
Cited
US5384261A
Cited
WO1993017126A1
Cited
US5412087A
Cited
US5554501A
Cited
US5580697A
Cited
US5472672A
Cited
US5529756A
Cited
WO1995011995A1
Cited
US5429807A
Cited
WO1995025116A1
Cited
WO1995035505A1
Cited
US5556752A
Cited
US5599695A
Cited
US5624711A
Cited
EP0742287A2
Cited
US5545531A
Cited
US5658734A
Cited
WO1997014706A1
Cited
EP0799897A1
Cited
WO1998030575A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2004-01-09 | 2004-07-10 | 2005-01-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2008-01-09 | 2008-07-10 | 2009-01-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2012-01-09 | 2012-07-10 | 2013-01-09 | $7700.00 | Window Closed / Lapsed |