US5658734A
Expired / Lapsed
US
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Process for synthesizing chemical compounds
Assignee: Affymetrix Inc
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Filing: 1995-10-17
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Granted: 1997-08-19
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Expires: 2015-10-17
Abstract
Abstract The present invention relates to a process for synthesizing on a single substrate a plurality of chemical compounds having diverse structures. The process involves the use of a bilayer photoresist to build up selected regions of the array in a step wise fashion.
Inventors
- 👤 Phillip Joe Brock
- 👤 William Dinan Hinsberg
- 👤 Jeffrey William Labadie
- 👤 Glenn McGall
- 👤 Gregory Michael Wallraff
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 18:29
CPC Classifications
B; B82; B82Y; B82Y30/00; B01; B01J; B01J19/00; B01J19/0046; C; C07; C07H; C07H21/00; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00351; B01J2219/00427; B01J2219/00432; B01J2219/00497; B01J2219/00527; B01J2219/00583; B01J2219/00585; B01J2219/0059; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/00612; B01J2219/00623; B01J2219/00626; B01J2219/00632; B01J2219/00637; B01J2219/00659; B01J2219/00709; B01J2219/00711; B01J2219/00718; B01J2219/0072; B01J2219/00722; B01J2219/00725; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B40/10; C40B60/00; C40B60/14; Y; Y10; Y10S; Y10S435/00; Y10S435/969; Y10S436/00; Y10S436/807; Y10S436/809; Y10S530/00; Y10S530/81
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
107 words
A process for synthesizing a plurality of chemical compounds having diverse structure on a substrate comprising the steps of: (a) coating a layer of protective polymer onto a layer of first molecules which are disposed on a substrate and have a labile protecting group; (b) coating a layer of radiation sensitive resist onto the layer of protective polymer; (c) imagewise exposing the resist layer to radiation; (d) developing the image to imagewise expose a portion of the layer of first molecules; (e) treating the exposed portion of the layer of first molecules to remove the protecting group; and (g) bonding second molecules to the exposed first molecules.
Claim 2
Dependent Claim
107 words
A process for synthesizing a plurality of chemical compounds having diverse structure on a substrate comprising the steps of: (a) coating a layer of protective polymer onto a layer of first molecules which are disposed on a substrate and have a labile protecting group; (b) coating a layer of radiation sensitive resist onto the layer of protective polymer; (c) imagewise exposing the resist layer to radiation; (d) developing the image to imagewise expose a portion of the layer of first molecules; (e) treating the exposed portion of the layer of first molecules to remove the protecting group; and (g) bonding second molecules to the exposed first molecules.
Claim 3
Dependent Claim
13 words
The process of claim 1 wherein the resist is a crosslinking negative resist.
Claim 4
Dependent Claim
12 words
The process of claim 2 wherein the resist is an epoxy resist.
Claim 5
Dependent Claim
14 words
The process of claim 2 wherein the protective polymer is polyimide or poly(vinyl alcohol).
Claim 6
Dependent Claim
107 words
A process for synthesizing a plurality of biochemical compounds having diverse structure on a substrate comprising the steps of: (a) coating a layer of protective polymer onto a layer of first molecules which are disposed on a substrate and have a labile protecting group; (b) coating a layer of radiation sensitive resist onto the layer of protective polymer; (c) imagewise exposing the resist layer to radiation; (d) developing the image to imagewise expose a portion of the layer of first molecules; (e) treating the exposed portion of the layer of first molecules to remove the protecting group; and (g) bonding second molecules to the exposed first molecules.
Claim 7
Dependent Claim
107 words
A process for synthesizing a plurality of biochemical compounds having diverse structure on a substrate comprising the steps of: (a) coating a layer of protective polymer onto a layer of first molecules which are disposed on a substrate and have a labile protecting group; (b) coating a layer of radiation sensitive resist onto the layer of protective polymer; (c) imagewise exposing the resist layer to radiation; (d) developing the image to imagewise expose a portion of the layer of first molecules; (e) treating the exposed portion of the layer of first molecules to remove the protecting group; and (g) bonding second molecules to the exposed first molecules.
Claim 8
Dependent Claim
14 words
The process of claim 5 wherein the polymeric resist is a crosslinking negative resist.
Claim 9
Dependent Claim
12 words
The process of claim 6 wherein the resist is an epoxy resist.
Claim 10
Dependent Claim
14 words
The process of claim 6 wherein the protective polymer is polyimide or poly(vinyl alcohol).
Claim 11
Dependent Claim
12 words
The process of claim 7 wherein the protective polymer is a polyimide.
Claim 12
Dependent Claim
19 words
The process of claim 5 wherein the layer of first molecules is a layer of nucleoside or nucleotide molecules.
Claim 13
Dependent Claim
17 words
The process of claim 5 wherein the second layer molecules are a layer of phosphoramidite nucleotide molecules.
Claim 14
Dependent Claim
15 words
The process of claim 11 wherein the nucleotide molecules are protected with dimethoxy trityl substituent.
Claim 15
Dependent Claim
27 words
The process of claim 11 wherein the nucleotide molecule is adenosine phosphate, guanosine phosphate, cytidine phosphate, uridine phosphate, deoxyadenosine phosphate, deoxyguanosine phosphate, deoxycytidine phosphate or deoxythymidine phosphate.
Claim 16
Dependent Claim
16 words
The process of claim 13 wherein the protecting group is triphenylmethyl, dimethoxy triphenylmethyl, t-butoxycarbonyloxy or fluorenylmethoxycarbonyl.
Claim 17
Dependent Claim
148 words
A process for screening unknown biochemical polymer for binding affinity comprising the steps of (a) exposing an array to biochemical polymers, the array comprising a plurality of biochemical compounds having diverse structure bonded to a substrate and made by the process of; (i) coating a layer of protective polymer onto a layer of first molecules which are disposed on a substrate and have a labile protecting group; (ii) coating a layer of radiation sensitive resist onto the layer of protective polymer; (iii) imagewise exposing the resist layer to radiation; (iv) developing the image to imagewise expose a portion of the layer of first molecules; (v) treating the exposed portion of the layer of first molecules to remove the protecting group; and (vi) bonding second molecules to the exposed first molecules; and (b) detecting on the array the locations of binding of the biochemical polymer to the biochemical compounds.
Claim 18
Dependent Claim
148 words
A process for screening unknown biochemical polymer for binding affinity comprising the steps of (a) exposing an array to biochemical polymers, the array comprising a plurality of biochemical compounds having diverse structure bonded to a substrate and made by the process of; (i) coating a layer of protective polymer onto a layer of first molecules which are disposed on a substrate and have a labile protecting group; (ii) coating a layer of radiation sensitive resist onto the layer of protective polymer; (iii) imagewise exposing the resist layer to radiation; (iv) developing the image to imagewise expose a portion of the layer of first molecules; (v) treating the exposed portion of the layer of first molecules to remove the protecting group; and (vi) bonding second molecules to the exposed first molecules; and (b) detecting on the array the locations of binding of the biochemical polymer to the biochemical compounds.
References Cited (Backward Citations)
US5143854A
Cited
WO1992010183A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-08-18 | 2001-02-17 | 2001-08-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-08-18 | 2005-02-17 | 2005-08-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-08-18 | 2009-02-17 | 2009-08-19 | $7700.00 | Window Closed / Lapsed |