US5429807A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for creating biopolymer arrays on a solid support surface
Assignee: Affymetrix Inc
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Filing: 1993-10-28
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Granted: 1995-07-04
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Expires: 2013-10-28
Abstract
Abstract An automated method and apparatus for performing biopolymer synthesis on a two-dimensional support surface whereby a two-dimensional matrix or array of biopolymers are obtained on the surface. An applicator in the form of a thick plate having in a surface at least one cavity in the form of an open chamber or channel is used for applying reagents to the surface of a solid phase support material. The applicator is positioned over the solid support material with the surface having the cavity sealed against the material surface. For each cavity, reagents for synthesis are introduced sequentially into one end of the cavity and collected from the other end of the cavity. A one-dimensional array of biopolymers are thus formed on the support material, where each element of the array contains a population of biopolymers having identical sequence. Further synthesis of additional biopolymers is performed by linearly moving or rotating the applicator relative to the support material and carrying out synthesis procedure so that another one-dimensional matrix or array of biopolymers are formed at an offset position to the previous array. At each overlapping region of the two arrays, a cell of biopolymers are formed which each comprises a strand from the first array and a strand from the second array. The result is a two-dimensional array of biopolymers of different sequences at each discrete cell. The arrays of discrete cell containing different biopolymers may be used to conduct hybridization reaction analysis of a biological sample.
Inventors
- 👤 Robert S. Matson
- 👤 Peter J. Coassin
- 👤 Jang B. Rampal
- 👤 Edwin M. Southern
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 21:16
CPC Classifications
C; C07; C07H; C07H21/00; B; B01; B01J; B01J19/00; B01J19/0046; C07K; C07K1/00; C07K1/04; C07K1/045; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6869; C12Q1/6874; G; G01; G01N; G01N35/00; G01N35/10; G01N35/1065; G01N35/1072; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00351; B01J2219/00427; B01J2219/0043; B01J2219/00497; B01J2219/00527; B01J2219/00583; B01J2219/0059; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/00612; B01J2219/00659; B01J2219/0068; B01J2219/00686; B01J2219/00718; B01J2219/0072; B01J2219/00722; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B60/00; C40B60/14
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
135 words
A device for synthesizing biopolymer comprising: a fixed polypropylene support having a surface activated for attaching bipolymers; an applicator for applying reagents to said activated surface, the applicator contacting said activated surface; positioning means for positioning the applicator with respect to the activated surface in first and second positions; reagent supply means for distributing reagents to said activated surface to synthesize a first one-dimensional array of biopolymers in said first position and a second one-dimensional array of biopolymers in said second position, the positioning means positioning the first and second positions such that said second one-dimensional array overlaps the first one-dimensional array to form a two-dimensional array of cells at overlapping regions on said surface in each of which a third biopolymer is obtained which includes corresponding biopolymer strands from said first and second arrays.
Claim 2
Independent Claim
135 words
A device for synthesizing biopolymer comprising: a fixed polypropylene support having a surface activated for attaching bipolymers; an applicator for applying reagents to said activated surface, the applicator contacting said activated surface; positioning means for positioning the applicator with respect to the activated surface in first and second positions; reagent supply means for distributing reagents to said activated surface to synthesize a first one-dimensional array of biopolymers in said first position and a second one-dimensional array of biopolymers in said second position, the positioning means positioning the first and second positions such that said second one-dimensional array overlaps the first one-dimensional array to form a two-dimensional array of cells at overlapping regions on said surface in each of which a third biopolymer is obtained which includes corresponding biopolymer strands from said first and second arrays.
Claim 3
Dependent Claim
26 words
A device as in claim 1 wherein said applicator includes a face having therein at least one cavity for flowing reagents for synthesis of the biopolymer.
Claim 4
Dependent Claim
48 words
A device as in claim 2 wherein the means for positioning includes means for sealing said cavity against said activated surface and flowing reagents for synthesis through the cavity such as to contact the activated surface with the reagents to allow reactions associated with synthesis to take place.
Claim 5
Dependent Claim
13 words
A device as in claim 3 wherein the cavity has a circular opening.
Claim 6
Dependent Claim
17 words
A device as in claim 3 wherein the cavity is in the shape of an elongated channel.
Claim 7
Dependent Claim
46 words
A device as in claim 2 wherein said applicator includes a face having a plurality of elongated channels having one end communicated to the reagent supply means, whereby the first and second arrays of biopolymers synthesize along regions on the activated surface corresponding to said channels.
Claim 8
Dependent Claim
61 words
A device as in claim 1 wherein said applicator includes a face having a plurality of elongated channels having one end communicated to the reagent supply means whereby the first and second arrays of biopolymers synthesize along regions on the activated surface corresponding to said channels, and the positioning means includes means for rotating the applicator relative to the activated surface.
Claim 9
Dependent Claim
33 words
A device as in claim 7 wherein the positioning means rotates the applicator by 90° relative to the activated surface such that the second one-dimensional array is orthogonal to the first one-dimensional array.
Claim 10
Dependent Claim
22 words
A device as in claim 1 wherein the positioning means includes means for moving the applicator linearly relative to the activated surface.
Claim 11
Dependent Claim
21 words
A device as in claim 1 wherein the positioning means includes means for rotating the applicator relative to the activated surface.
Claim 12
Independent Claim
128 words
A method for synthesizing biopolymer comprising the steps of: providing a fixed polypropylene support having a surface activated for attaching biopolymers; providing an applicator in contact with said activated surface for applying reagents to said activated surface; positioning the applicator with respect to the activated surface and applying reagents to said activated surface to synthesize a first one-dimensional array of biopolymers; repositioning the applicator and applying reagents to said activated surface to synthesize a second one-dimensional array of biopolymers in a manner where said second one-dimensional array overlaps the first one-dimensional array to form a two-dimensional array of cells at overlapping regions on the activated surface in each of which a third biopolymer is obtained which includes a corresponding biopolymer from said first and second arrays of biopolymers.
Claim 13
Independent Claim
128 words
A method for synthesizing biopolymer comprising the steps of: providing a fixed polypropylene support having a surface activated for attaching biopolymers; providing an applicator in contact with said activated surface for applying reagents to said activated surface; positioning the applicator with respect to the activated surface and applying reagents to said activated surface to synthesize a first one-dimensional array of biopolymers; repositioning the applicator and applying reagents to said activated surface to synthesize a second one-dimensional array of biopolymers in a manner where said second one-dimensional array overlaps the first one-dimensional array to form a two-dimensional array of cells at overlapping regions on the activated surface in each of which a third biopolymer is obtained which includes a corresponding biopolymer from said first and second arrays of biopolymers.
Claim 14
Dependent Claim
71 words
A method as in claim 11 wherein said applicator provided includes a face having therein at least one cavity for flowing reagents for synthesis of the biopolymer, and the positioning and repositioning steps include the steps of: sealing said cavity against said activated surface; and flowing reagents for synthesis through the cavity such as to contact the activated surface with the reagents to allow reactions associated with synthesis to take place.
Claim 15
Dependent Claim
13 words
A method as in claim 12 wherein the cavity has a circular opening.
Claim 16
Dependent Claim
17 words
A method as in claim 12 wherein the cavity is in the shape of an elongated channel.
Claim 17
Dependent Claim
46 words
A method as in claim 12 wherein said applicator includes a face having a plurality of elongated channels having one end communicated to the reagent supply means, whereby the first and second arrays of biopolymers synthesize along regions on the activated surface corresponding to said channels.
Claim 18
Dependent Claim
62 words
A method as in claim 11 wherein said applicator includes a face having a plurality of elongated channels having one end communicated to the reagent supply means whereby the first and second arrays of biopolymers synthesize along regions on the activated surface corresponding to said channels, and the positioning step includes the step of rotating the applicator relative to the activated surface.
Claim 19
Dependent Claim
31 words
A method as in claim 16 wherein the applicator is rotated by 90° relative to the activated surface such that the second one-dimensional array is orthogonal to the first one-dimensional array.
Claim 20
Dependent Claim
23 words
A method as in claim 11 wherein the positioning step includes the step of moving the applicator linearly relative to the activated surface.
Claim 21
Dependent Claim
22 words
A method as in claim 11 wherein the positioning step includes the step of rotating the applicator relative to the activated surface.
Claim 22
Independent Claim
130 words
A method for synthesizing polynucleotides comprising the steps of: providing a fixed polypropylene support having a surface activated for attaching polynucleotides; providing an applicator in contact with said activated surface for applying reagents suitable for polynucleotide synthesis to said activated surface; positioning the applicator with respect to the activated surface and applying reagents to said activated surface to synthesize a first one-dimensional array of polynucleotides; repositioning the applicator and applying reagents to said activated surface to synthesize a second one-dimensional array of polynucleotides in a manner where said second one-dimensional array overlaps the first one-dimensional array to form a two-dimensional array of cells at overlapping regions on the activated surface in each of which a third polynucleotide is obtained which includes corresponding polynucleotide strands from said first and second arrays.
Claim 23
Independent Claim
130 words
A method for synthesizing polynucleotides comprising the steps of: providing a fixed polypropylene support having a surface activated for attaching polynucleotides; providing an applicator in contact with said activated surface for applying reagents suitable for polynucleotide synthesis to said activated surface; positioning the applicator with respect to the activated surface and applying reagents to said activated surface to synthesize a first one-dimensional array of polynucleotides; repositioning the applicator and applying reagents to said activated surface to synthesize a second one-dimensional array of polynucleotides in a manner where said second one-dimensional array overlaps the first one-dimensional array to form a two-dimensional array of cells at overlapping regions on the activated surface in each of which a third polynucleotide is obtained which includes corresponding polynucleotide strands from said first and second arrays.
Claim 24
Independent Claim
227 words
A device for synthesizing biopolymer comprising: a support material having a surface activated for attaching biopolymers; an applicator for applying reagents to said activated surface, the applicator contacting said activated surface; reagent supply means for distributing reagents to said activated surface to synthesize a first one-dimensional array of biopolymers in a first position and a second one-dimensional array of biopolymers in a second position, the reagent supply means including: a primary rotary valve with a plurality of ports, a plurality of secondary rotary valves each with a plurality of ports, each secondary rotary valve connected in parallel with respect to the other secondary rotary valves and the primary and secondary rotary valves connected in series, the ports of the primary and secondary rotary valves being capable of variable alignment, and a controller for selective operation of the primary and secondary rotary valves resulting in selective reagent distribution to the applicator in a sequence-specific manner, and positioning means for positioning the applicator with respect to the activated surface in the first and the second positions, the positioning means positioning the first and second positions such that said second one-dimensional array overlaps the first one-dimensional array to form a two-dimensional array of cells at overlapping regions on said surface in each of which a third biopolymer is obtained which includes corresponding biopolymer strands from said first and second arrays.
Claim 25
Independent Claim
227 words
A device for synthesizing biopolymer comprising: a support material having a surface activated for attaching biopolymers; an applicator for applying reagents to said activated surface, the applicator contacting said activated surface; reagent supply means for distributing reagents to said activated surface to synthesize a first one-dimensional array of biopolymers in a first position and a second one-dimensional array of biopolymers in a second position, the reagent supply means including: a primary rotary valve with a plurality of ports, a plurality of secondary rotary valves each with a plurality of ports, each secondary rotary valve connected in parallel with respect to the other secondary rotary valves and the primary and secondary rotary valves connected in series, the ports of the primary and secondary rotary valves being capable of variable alignment, and a controller for selective operation of the primary and secondary rotary valves resulting in selective reagent distribution to the applicator in a sequence-specific manner, and positioning means for positioning the applicator with respect to the activated surface in the first and the second positions, the positioning means positioning the first and second positions such that said second one-dimensional array overlaps the first one-dimensional array to form a two-dimensional array of cells at overlapping regions on said surface in each of which a third biopolymer is obtained which includes corresponding biopolymer strands from said first and second arrays.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-07-03 | 1999-01-02 | 1999-07-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-07-03 | 2003-01-02 | 2003-07-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-07-03 | 2007-01-02 | 2007-07-04 | $7700.00 | Window Closed / Lapsed |