US6111165A
Expired / Lapsed
US
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Production of human recombinant collagen in the milk of transgenic animals
Assignee: Pharming BV
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Filing: 1999-01-15
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Granted: 2000-08-29
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Expires: 2019-01-15
Abstract
Abstract Production of human procollagen or collagen in cells which ordinarily do not produce these molecules is effected by constructing expression systems compatible with mammary glands of non-human mammals. For example, expression systems can be microinjected into fertilized oocytes and reimplanted in foster mothers and carried to term in order to obtain transgenic non-human mammals capable of producing milk containing recombinant human procollagen or collagen. Human procollagen or collagen produced in this manner can be made of a single collagen type uncontaminated by other human or non-human collagens.
Inventors
- 👤 Richard A. Berg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 06:28
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/8509; A; A01; A01K; A01K67/00; A01K67/027; A01K67/0275; A01K67/0278; A23; A23C; A23C9/00; A23C9/20; A23J; A23J1/00; A23J1/20; A23J3/00; A23J3/04; A23J3/06; C07; C07K; C07K14/00; C07K14/435; C07K14/78; A01K2207/00; A01K2207/15; A01K2217/00; A01K2217/05; A01K2227/00; A01K2227/10; A01K2227/105; A01K2267/00; A01K2267/01; A23C2230/00; A23C2230/05; Y; Y10; Y10S; Y10S119/00; Y10S119/01; Y10S530/00; Y10S530/827; Y10S530/832
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
116 words
A method of obtaining milk, comprising the step of: recovering milk from a transgenic nonhuman mammal whose somatic and germ cells contain an expression system comprising a coding nucleotide sequence encoding a human procollagen polypeptide chain operably linked to a control nucleotide sequence that effects expression specifically in milk protein-secreting epithelial cells of a mammary gland in said mammal, and said cells express said coding nucleotide sequence to produce said polypeptide chain, and secrete a single type of human procollagen, collagen, or a mixture thereof into the milk of said mammal, wherein said milk comprises trimeric collagen, procollagen or mixture thereof in a recoverable amount without extracellular aggregation which would prevent excretion from said mammary gland.
Claim 2
Independent Claim
116 words
A method of obtaining milk, comprising the step of: recovering milk from a transgenic nonhuman mammal whose somatic and germ cells contain an expression system comprising a coding nucleotide sequence encoding a human procollagen polypeptide chain operably linked to a control nucleotide sequence that effects expression specifically in milk protein-secreting epithelial cells of a mammary gland in said mammal, and said cells express said coding nucleotide sequence to produce said polypeptide chain, and secrete a single type of human procollagen, collagen, or a mixture thereof into the milk of said mammal, wherein said milk comprises trimeric collagen, procollagen or mixture thereof in a recoverable amount without extracellular aggregation which would prevent excretion from said mammary gland.
Claim 3
Dependent Claim
29 words
The method of claim 1, wherein said single type of human procollagen is human Type I procollagen and said single type of human collagen is human Type I collagen.
Claim 4
Dependent Claim
14 words
The method of claim 1, wherein said promoter is a bovine alpha S1-casein promoter.
Claim 5
Dependent Claim
16 words
The method of claim 1, wherein said mammal is selected from mouse, cow, sheep and goat.
Claim 6
Dependent Claim
106 words
Milk from a transgenic nonhuman mammal whose somatic and germ cells contain an expression system comprising a coding nucleotide sequence encoding a human procollagen polypeptide chain operably linked to a control nucleotide sequence that effects expression specifically in milk protein-secreting epithelial cells of a mammary gland in said mammal, and said cells express said coding nucleotide sequence to produce said polypeptide chain, and secrete a single type of human procollagen, collagen, or a mixture thereof into the milk of said mammal, wherein said milk comprises trimeric collagen, procollagen or mixture thereof in a recoverable amount without extracellular aggregation which would prevent excretion from said mammary gland.
Claim 7
Dependent Claim
106 words
Milk from a transgenic nonhuman mammal whose somatic and germ cells contain an expression system comprising a coding nucleotide sequence encoding a human procollagen polypeptide chain operably linked to a control nucleotide sequence that effects expression specifically in milk protein-secreting epithelial cells of a mammary gland in said mammal, and said cells express said coding nucleotide sequence to produce said polypeptide chain, and secrete a single type of human procollagen, collagen, or a mixture thereof into the milk of said mammal, wherein said milk comprises trimeric collagen, procollagen or mixture thereof in a recoverable amount without extracellular aggregation which would prevent excretion from said mammary gland.
Claim 8
Dependent Claim
33 words
Milk from the transgenic nonhuman mammal of claim 5, wherein said single type of human procollagen is human Type I procollagen and said single type of human collagen is human Type I collagen.
Claim 9
Dependent Claim
18 words
Milk from the transgenic nonhuman mammal of claim 5, wherein said promoter is a bovine alpha S1-casein promoter.
Claim 10
Dependent Claim
20 words
Milk from the transgenic nonhuman mammal of claim 5, wherein said mammal is selected from mouse, cow, sheep and goat.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-08-29 | 2004-02-28 | 2004-08-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-08-29 | 2008-02-28 | 2008-08-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-08-29 | 2012-02-28 | 2012-08-29 | $7700.00 | Window Closed / Lapsed |