US4873316A
Expired / Lapsed
US
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Isolation of exogenous recombinant proteins from the milk of transgenic mammals
Assignee: Pharming Intellectual Property BV
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Filing: 1987-06-23
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Granted: 1989-10-10
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Expires: 2007-06-23
Abstract
Abstract This invention relates to the production of recombinant proteins in mammals' milk. Particularly, this invention relates to an expression system comprising the mammal's casein promoter which when transgenically incorporated into a mammal permits the female species of that mammal to produce the desired recombinant protein in or along with its milk. This invention also relates to the transgenic mammal that produces the desired recombinant product in its milk.
Inventors
- 👤 Harry Meade
- 👤 Nils Lonberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 20:07
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/8509; A; A01; A01K; A01K67/00; A01K67/027; A01K67/0275; A01K67/0278; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4732; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; A01K2207/00; A01K2207/15; A01K2217/00; A01K2217/05; A01K2227/00; A01K2227/10; A01K2227/103; A01K2227/105; A01K2267/00; A01K2267/01; C07K2319/00; C07K2319/01; C07K2319/02; C12N2830/00; C12N2830/008; C12N2830/80; C12N2830/85; C12N2840/00; C12N2840/44; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/832; Y10S530/833
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
81 words
A process for the production and secretion into mammal's milk of an exogenous recombinate protein comprising the steps of: a. producing milk in a transgenic mammal characterized by an expression system comprising a casein promoter operatively linked to an exogenous DNA sequence coding for the recombinant protein through a DNA sequence coding for a signal peptide effective in secreting and maturing the recombinant protein in mammary tissue; b. collecting the milk; and c. isolating the exogenous recombinant protein from the milk.
Claim 2
Dependent Claim
81 words
A process for the production and secretion into mammal's milk of an exogenous recombinate protein comprising the steps of: a. producing milk in a transgenic mammal characterized by an expression system comprising a casein promoter operatively linked to an exogenous DNA sequence coding for the recombinant protein through a DNA sequence coding for a signal peptide effective in secreting and maturing the recombinant protein in mammary tissue; b. collecting the milk; and c. isolating the exogenous recombinant protein from the milk.
Claim 3
Dependent Claim
26 words
The process according to claim 1, wherein said expression system also includes a 3' untranslated region downstream of the DNA sequence coding for the recombinant protein.
Claim 4
Dependent Claim
28 words
The process according to claim 1, wherein said expression system also includes a 5' untranslated region between said promoter and the DNA sequence coding for the signal peptide.
References Cited (Backward Citations)
US4018752A
Cited
US4229342A
Cited
US4396601A
Cited
US4376072A
Cited
EP0117059A2
Cited
US4462932A
Cited
US4736866B1
Cited
US4736866A
Cited
US4644056A
Cited
EP0264166B1
Cited
EP0247494A2
Cited
WO1988000239A1
Cited
WO1988001648A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-10-09 | 1993-04-10 | 1993-10-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-10-09 | 1997-04-10 | 1997-10-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-10-09 | 2001-04-10 | 2001-10-10 | $7700.00 | Window Closed / Lapsed |