US4462932A
Expired / Lapsed
US
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Isolation of native casein by cryodestabilization
Assignee: Purdue Research Foundation
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Filing: 1983-06-22
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Granted: 1984-07-31
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Expires: 2003-06-22
Abstract
Abstract A casein isolate which is substantially dispersible in water is disclosed along with the method of isolating this dispersible casein from skim milk. To isolate the casein, skim milk is filtered through a membrane of a type through which casein generally does not pass to concentrate the retentate to about a 4X VCR concentration. The retentate is stored at a temperature below the freezing point of the retentate for about four days. The retentate is diluted with a suitable diluent, such as water, in an amount sufficient to prevent gelling of the retentate. The casein is then substantially separated from the diluent and other materials in the retentate. Normally, this separation is performed by centrifugation.
Inventors
- 👤 Dennis A. Lonergan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 21:05
CPC Classifications
A; A23; A23J; A23J1/00; A23J1/20; A23J1/202; A23C; A23C9/00; A23C9/14; A23C9/142; A23C9/1422; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/832
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
79 words
A method for processing milk to isolate substantially all of the native casein therefrom in a form capable of forming a stable suspension in water, which comprises the steps of filtering milk through a membrane which substantially completely retains native casein while generally allowing lactose and water to pass through, withdrawing the retentate, storing the retentate at a temperature below the freezing point thereof, thawing the retentate, and separating the native casein therefrom in the presence of added water.
Claim 2
Independent Claim
79 words
A method for processing milk to isolate substantially all of the native casein therefrom in a form capable of forming a stable suspension in water, which comprises the steps of filtering milk through a membrane which substantially completely retains native casein while generally allowing lactose and water to pass through, withdrawing the retentate, storing the retentate at a temperature below the freezing point thereof, thawing the retentate, and separating the native casein therefrom in the presence of added water.
Claim 3
Dependent Claim
25 words
The method of claim 1 wherein the step of filtering the milk comprises the step of filtering the milk to at least about 2X VCR.
Claim 4
Dependent Claim
25 words
The method of claim 1 wherein the step of filtering the milk comprises the step of filtering the milk to at least about 4X VCR.
Claim 5
Dependent Claim
26 words
The method of claim 1 wherein the step of filtering the milk comprises the step of filtering the milk to between about 4X and 6X VCR.
Claim 6
Dependent Claim
21 words
The method of claim 1 wherein the step of filtering the milk comprises the step of filtering the milk by ultrafiltration.
Claim 7
Dependent Claim
21 words
The method of claim 1 wherein the step of filtering the milk comprises the step of filtering the milk by diafiltration.
Claim 8
Dependent Claim
25 words
The method of claim 1 wherein the step of storing the retentate comprises the step of storing the retentate for at least about four days.
Claim 9
Dependent Claim
27 words
The method of claim 1 wherein the step of storing the retentate comprises the step of storing the retentate for between about four days and four weeks.
Claim 10
Dependent Claim
23 words
The method of claim 1 wherein the step of storing the retentate comprises the step of storing the retentate at about -8° C.
Claim 11
Dependent Claim
37 words
The method of claim 1 wherein the step of separating the native casein in the presence of added water includes the step of adding water to the retentate in amounts sufficient to prevent gelling of the retentate.
Claim 12
Dependent Claim
18 words
The method of claim 10 wherein the step of adding water is performed before the retentate is stored.
Claim 13
Dependent Claim
18 words
The method of claim 10 wherein the step of adding water is performed after the retentate is stored.
Claim 14
Dependent Claim
31 words
The method of claim 10 wherein the step of adding water comprises the step of adding a sufficient quantity of water to bring the retentate to at least about 2X VCR.
Claim 15
Dependent Claim
27 words
The method of claim 1 wherein the step of substantially separating the native casein in the presence of added water comprises the step of centrifuging the retentate.
Claim 16
Dependent Claim
18 words
The method of claim 1 wherein the step of filtering milk comprises the step of filtering skim milk.
Claim 17
Independent Claim
97 words
A method of isolating native casein from milk comprising the steps of filtering milk through a membrane of a type through which casein generally will not pass to concentrate the milk filtered to at least about 2X VCR, saving the retentate from the filtration process, storing the retentate for at least about four days at a temperature below the freezing point of the retentate, thawing the retentate, diluting the retentate with a suitable diluent in amounts sufficient to prevent gelling of the retentate, and substantially separating the casein from the diluent and other materials in the retentate.
Claim 18
Independent Claim
97 words
A method of isolating native casein from milk comprising the steps of filtering milk through a membrane of a type through which casein generally will not pass to concentrate the milk filtered to at least about 2X VCR, saving the retentate from the filtration process, storing the retentate for at least about four days at a temperature below the freezing point of the retentate, thawing the retentate, diluting the retentate with a suitable diluent in amounts sufficient to prevent gelling of the retentate, and substantially separating the casein from the diluent and other materials in the retentate.
Claim 19
Dependent Claim
83 words
A native casein isolate which is substantially dispersible in water, the casein being isolated from milk by a process which essentially completely isolates the casein from the milk, the process comprising the steps of filtering milk through a membrane which substantially completely retains native casein while generally allowing lactose and water to pass through, withdrawing the retentate, storing the retentate at a temperature below the freezing point thereof, thawing the retentate, and separating the native casein therefrom in the presence of added water.
Claim 20
Dependent Claim
83 words
A native casein isolate which is substantially dispersible in water, the casein being isolated from milk by a process which essentially completely isolates the casein from the milk, the process comprising the steps of filtering milk through a membrane which substantially completely retains native casein while generally allowing lactose and water to pass through, withdrawing the retentate, storing the retentate at a temperature below the freezing point thereof, thawing the retentate, and separating the native casein therefrom in the presence of added water.
Claim 21
Dependent Claim
25 words
The composition of claim 17 wherein the step of filtering the milk comprises the step of filtering the milk to at least about 2X VCR.
Claim 22
Dependent Claim
25 words
The composition of claim 17 wherein the step of filtering the milk comprises the step of filtering the milk to at least about 4X VCR.
Claim 23
Dependent Claim
26 words
The composition of claim 17 wherein the step of filtering the milk comprises the step of filtering the milk to between about 4X and 6X VCR.
Claim 24
Dependent Claim
21 words
The composition of claim 1 wherein the step of filtering the milk comprises the step of filtering the milk by ultrafiltration.
Claim 25
Dependent Claim
21 words
The composition of claim 1 wherein the step of filtering the milk comprises the step of filtering the milk by diafiltration.
Claim 26
Dependent Claim
25 words
The composition of claim 1 wherein the step of storing the retentate comprises the step of storing the retentate for at least about four days.
Claim 27
Dependent Claim
27 words
The composition of claim 1 wherein the step of storing the retentate comprises the step of storing the retentate for between about four days and four weeks.
Claim 28
Dependent Claim
23 words
The composition of claim 1 wherein the step of storing the retentate comprises the step of storing the retentate at about -8° C.
Claim 29
Dependent Claim
37 words
The composition of claim 18 wherein the step of separating the native casein in the presence of added water includes the step of adding water to the retentate in amounts sufficient to prevent gelling of the retentate.
Claim 30
Dependent Claim
18 words
The composition of claim 26 wherein the step of adding water is performed before the retentate is stored.
Claim 31
Dependent Claim
18 words
The composition of claim 26 wherein the step of adding water is performed after the retentate is stored.
Claim 32
Dependent Claim
31 words
The composition of claim 26 wherein the step of adding water comprises the step of adding a sufficient quantity of water to bring the retentate to at least about 2X VCR.
Claim 33
Dependent Claim
27 words
The composition of claim 17 wherein the step of substantially separating the native casein in the presence of added water comprises the step of centrifuging the retentate.
Claim 34
Dependent Claim
18 words
The composition of claim 1 wherein the step of filtering milk comprises the step of filtering skim milk.
Claim 35
Dependent Claim
103 words
A native casein isolate which is substantially dispersible in water, the casein being isolated from milk by a process comprising the steps of filtering the milk through a membrane through which casein generally will not pass to concentrate the milk filtered to at least about 2X VCR, storing the retentate from the filtration process for at least about four days at a temperature below the freezing point of the retentate, thawing the retentate, diluting the retentate with a suitable diluent in amounts sufficient to prevent gelling of the retentate, and substantially separating the casein from the diluent and other materials in the retentate.
Claim 36
Dependent Claim
103 words
A native casein isolate which is substantially dispersible in water, the casein being isolated from milk by a process comprising the steps of filtering the milk through a membrane through which casein generally will not pass to concentrate the milk filtered to at least about 2X VCR, storing the retentate from the filtration process for at least about four days at a temperature below the freezing point of the retentate, thawing the retentate, diluting the retentate with a suitable diluent in amounts sufficient to prevent gelling of the retentate, and substantially separating the casein from the diluent and other materials in the retentate.
Claim 37
Independent Claim
112 words
A method of isolating native casein from skim milk comprising the steps of first ultrafiltering skim milk through a membrane of a type through which casein generally will not pass to concentrate the milk filtered to between about 4X and 6X VCR, then saving the retentate from the filtration process, then storing the retentate for between about four days and four weeks at a temperature below the freezing point of the retentate, then thawing the retentate, then diluting the retentate with water in amounts sufficient to bring the retentate to at least about 2X VCR, and then substantially separating the casein from the diluent and other materials in the retentate by centrifugation.
Claim 38
Independent Claim
112 words
A method of isolating native casein from skim milk comprising the steps of first ultrafiltering skim milk through a membrane of a type through which casein generally will not pass to concentrate the milk filtered to between about 4X and 6X VCR, then saving the retentate from the filtration process, then storing the retentate for between about four days and four weeks at a temperature below the freezing point of the retentate, then thawing the retentate, then diluting the retentate with water in amounts sufficient to bring the retentate to at least about 2X VCR, and then substantially separating the casein from the diluent and other materials in the retentate by centrifugation.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-07-31 | 1988-01-30 | 1988-07-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-07-31 | 1992-01-30 | 1992-07-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-07-31 | 1996-01-30 | 1996-07-31 | $7700.00 | Window Closed / Lapsed |