EP0264166B1
Expired / Lapsed
EP
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Transgenic animals secreting desired proteins into milk
Assignee: Genzyme Corp
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Filing: 1987-04-09
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Granted: 1996-08-21
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Expires: 2007-04-09
Abstract
Abstract A DNA sequence contains a gene encoding a protein, the gene being under the transcriptional control i n the DNA sequence of a mammalian milk protein promoter which does not naturally control the transcription of the gene, such DNA sequence including DNA enabling secretion of the protein.
Inventors
- 👤 Katherine Gordon
- 👤 Suzanne Groet
- 👤 Lothar Hennighausen
- 👤 Heiner Westphal Of
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 16:14
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/8509; C07; C07K; C07K14/00; C07K14/005; C12N15/87; C12N15/89; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6459; C12Y; C12Y304/00; C12Y304/21; C12Y304/21069; A; A01; A01K; A01K2207/00; A01K2207/15; A01K2217/00; A01K2217/05; A01K2227/00; A01K2227/10; A01K2267/00; A01K2267/01; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122; C12N2830/00; C12N2830/008; C12N2830/80; C12N2830/85
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
50 words
A DNA sequence containing a gene encoding a protein, said gene being under the transcriptional control in said DNA sequence of a mammalian casein or milk serum protein promoter which does not naturally control the transcription of said gene, said DNA sequence further comprising DNA enabling secretion of said protein.
Claim 2
Dependent Claim
22 words
The DNA sequence of Claim 1, wherein said secretionenabling DNA comprises a secretion signal-encoding sequence interposed between said gene and said promoter.
Claim 3
Dependent Claim
16 words
The DNA sequence of Claim 1 wherein said milk serum protein is a whey acid protein.
Claim 4
Dependent Claim
24 words
The DNA sequence of Claim 2 wherein said signal encoding sequence is the signal encoding sequence naturally associated with said gene encoding said protein.
Claim 5
Dependent Claim
27 words
The DNA sequence of Claim 2 wherein said signal encoding sequence is the signal encoding sequence naturally associated with said mammalian casein or milk serum protein promoter.
Claim 6
Dependent Claim
15 words
The DNA sequence of Claim 1 wherein said DNA sequence includes a transcriptional stop sequence.
Claim 7
Dependent Claim
16 words
The DNA sequence of Claim 6 wherein said stop sequence is derived from SV40 virus DNA.
Claim 8
Dependent Claim
18 words
The DNA sequence of Claim 6 wherein said stop sequence is contained in the polyadenylation sequence of SV40.
Claim 9
Dependent Claim
19 words
The DNA sequence of Claim 1 wherein said protein is human tissue plasminogen activator or hepatitis B surface antigen.
Claim 10
Independent Claim
116 words
A method for producing a protein comprising the steps of: (a) inserting into a non-human mammalian embryo a DNA sequence comprising a gene encoding said protein, said gene being under the transcriptional control of a casein or milk serum protein promoter which does not naturally control the transcription of said gene, said DNA sequence comprising DNA enabling secretion of said protein, (b) allowing said embryo to develop into an adult mammal, (c) inducing lactation in said mammal, or in a female descendant of said mammal in which said gene, promoter, and signal sequence are present in the mammary tissue genome, (d) collecting milk of said lactating mammal, and (e) isolating said protein from said collected milk.
References Cited (Backward Citations)
US4873316A
Cited
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