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US4873191A
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Genetic transformation of zygotes
Assignee: Ohio University
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Filing: 1986-08-18
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Granted: 1989-10-10
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Expires: 2006-08-18
Abstract
Abstract Genetic transformation of a zygote and the embryo and mature organism which result therefrom is obtained by placing or inserting exogenous genetic material into the nucleus of the zygote or into any genetic material which ultimately forms at least a part of the nucleus of the zygote. It is preferred that the exogenous genetic material be added to a pronuclei of the zygote and is particularly preferred that it be added to the male pronucleus of the zygote. Thereafter, the zygote is allowed to undergo differentiation and development into the organism. The genotype of the zygote and the organism which results therefrom will include the genotype of the exogenous genetic material and the exogenous genetic material will be phenotypically expressed. The invention can be utilized in a variety of ways including, for example, animal and plant breeding to modify or create new species, it can be used in epigenetics and in the understanding and treatment of genetic diseases.
Inventors
- 👤 Thomas E. Wagner
- 👤 Peter C. Hoppe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 17:17
CPC Classifications
A; A01; A01K; A01K67/00; A01K67/027; A01K67/0275; A01K2217/00; A01K2217/05
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
157 words
A method of obtaning a mammal characterized as having a plurality of cells containing exogenous genetic material, said material including at least one gene and a control sequence operably associated therewith, which, under predetermined conditions, express said gene under the control of said control sequence in a cell of said mammal, which comprises: (a) introducing exogenous genetic material into a pronucleus of a mammalian zygote by microinjection, said zygote being capable of development into a mammal, said genetic material including at least one gene and a control sequence operably associated therewith, thereby obtaining a genetically transformed zygote; (b) transplanting an embryo derived from the genetically transformed zygote into a pseudopregnant female capable of bearing the embryo to term; and (c) allowing the embryo to develop to term; where said gene and control sequence are selected so that the gene is not activated in such manner and degree as would prevent normal development of the embryo to term.
Claim 2
Independent Claim
157 words
A method of obtaning a mammal characterized as having a plurality of cells containing exogenous genetic material, said material including at least one gene and a control sequence operably associated therewith, which, under predetermined conditions, express said gene under the control of said control sequence in a cell of said mammal, which comprises: (a) introducing exogenous genetic material into a pronucleus of a mammalian zygote by microinjection, said zygote being capable of development into a mammal, said genetic material including at least one gene and a control sequence operably associated therewith, thereby obtaining a genetically transformed zygote; (b) transplanting an embryo derived from the genetically transformed zygote into a pseudopregnant female capable of bearing the embryo to term; and (c) allowing the embryo to develop to term; where said gene and control sequence are selected so that the gene is not activated in such manner and degree as would prevent normal development of the embryo to term.
Claim 3
Dependent Claim
16 words
The method of claim 1 wherein the control sequence is one naturally associated with the gene.
Claim 4
Dependent Claim
31 words
The method of claim 1 wherein the control sequence is one which will activate the gene when the cell is exposed to stimulus different from the natural stimulus of the gene.
Claim 5
Dependent Claim
22 words
The method of claim 1 wherein the control sequence is responsive to the same stimuli as is the beta globin control sequence.
Claim 6
Dependent Claim
33 words
The method of claim 1 in which at least about 1,000 copies of the gene are microinjected into the pronucleus and the volume of genetic material injected does not exceed about 10 picoliters.
Claim 7
Dependent Claim
25 words
The method of claim 1 in which the material is introduced into the male pronucleus of an egg having a separate female and male pronucleus.
Claim 8
Dependent Claim
24 words
The method of claim 1 in which the zygote is allowed to develop in vitro to the morula or blastocyst stage prior to transplantation.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-10-09 | 1993-04-10 | 1993-10-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-10-09 | 1997-04-10 | 1997-10-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-10-09 | 2001-04-10 | 2001-10-10 | $7700.00 | Window Closed / Lapsed |