EP0264166A1
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Transgenic animals secreting desired proteins into milk
Assignee: Genzyme Corp
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Filing: 1987-04-09
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Granted: —
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Expires: 2007-04-09
Abstract
Abstract A DNA sequence contains a gene encoding a protein, the gene being under the transcriptional control i n the DNA sequence of a mammalian milk protein promoter which does not naturally control the transcription of the gene, such DNA sequence including DNA enabling secretion of the protein.
Inventors
- 👤 Katherine Gordon
- 👤 Suzanne Groet
- 👤 Lothar Hennighausen
- 👤 Heiner Westphal Of
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 13:44
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/8509; C07; C07K; C07K14/00; C07K14/005; C12N15/87; C12N15/89; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6459; C12Y; C12Y304/00; C12Y304/21; C12Y304/21069; A; A01; A01K; A01K2207/00; A01K2207/15; A01K2217/00; A01K2217/05; A01K2227/00; A01K2227/10; A01K2267/00; A01K2267/01; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122; C12N2830/00; C12N2830/008; C12N2830/80; C12N2830/85
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
47 words
A DNA sequence containing a gene encoding a protein, said gene being under the transcriptional control in said DNA sequence of a mammalian milk protein promoter which does not naturally control the transcription of said gene, said DNA sequence further comprising DNA enabling secretion of said protein.
Claim 2
Dependent Claim
47 words
A DNA sequence containing a gene encoding a protein, said gene being under the transcriptional control in said DNA sequence of a mammalian milk protein promoter which does not naturally control the transcription of said gene, said DNA sequence further comprising DNA enabling secretion of said protein.
Claim 3
Dependent Claim
22 words
The DNA sequence of claim l, wherein said secretion-enabling DNA comprises a secretion signal-encoding sequence interposed between said gene and said promoter.
Claim 4
Dependent Claim
19 words
The DNA sequence of claim l wherein said milk protein is a milk serum protein or a casein protein.
Claim 5
Dependent Claim
16 words
The DNA sequence of claim 3 wherein said milk serum protein is a whey acid protein.
Claim 6
Dependent Claim
24 words
The DNA sequence of claim l wherein said signal encoding sequence is the signal encoding sequence naturally associated with said gene encoding said protein.
Claim 7
Dependent Claim
24 words
The DNA sequence of claim l wherein said signal encoding sequence is the signal encoding sequence naturally associated with said mammalian milk protein promoter.
Claim 8
Dependent Claim
15 words
The DNA sequence of claim l wherein said DNA sequence includes a transcriptional stop sequence.
Claim 9
Dependent Claim
16 words
The DNA sequence of claim 7 wherein said stop sequence is derived from SV40 virus DNA.
Claim 10
Dependent Claim
18 words
The DNA sequence of claim 7 wherein said stop sequence is contained in the polyadenylation sequence of SV40.
Claim 11
Dependent Claim
14 words
l0. A mammalian embryo having a nucleus containing the DNA sequence of claim l.
Claim 12
Dependent Claim
19 words
The DNA sequence of claim l wherein said protein is human tissue plasminogen activator or hepatitis B surface antigen.
Claim 13
Dependent Claim
55 words
A mammal in which the genome of the mammary glands of said mammal comprises a gene encoding a protein, said gene being under the transcriptional control of a mammalian milk protein promoter which does not naturally control the transcription of said gene, said genome comprising DNA enabling the secretion of said gene encoding said protein.
Claim 14
Dependent Claim
55 words
A mammal in which the genome of the mammary glands of said mammal comprises a gene encoding a protein, said gene being under the transcriptional control of a mammalian milk protein promoter which does not naturally control the transcription of said gene, said genome comprising DNA enabling the secretion of said gene encoding said protein.
Claim 15
Dependent Claim
16 words
The mammal of claim ll, said mammal being a sheep, pig, goat, cow, or other mammals.
Claim 16
Dependent Claim
18 words
The mammal of claim ll wherein said gene is expressed in greater amounts during lactation than during pregnancy.
Claim 17
Independent Claim
112 words
A method for producing a protein comprising the steps of: (a) inserting into a mammalian embryo a DNA sequence comprising a gene encoding said protein, said gene being under the transcriptional control of a milk protein promoter which does not naturally control the transcription of said gene, said DNA sequence comprising DNA enabling secretion of said protein, (b) allowing said embryo to develop into an adult mammal, (c) inducing lactation in said mammal, or in a female descendant of said mammal in which said gene, promoter, and signal sequence are present in the mammary tissue genome, (d) collecting milk of said lactating mammal, and (e) isolating said protein from said collected milk.
Claim 18
Independent Claim
112 words
A method for producing a protein comprising the steps of: (a) inserting into a mammalian embryo a DNA sequence comprising a gene encoding said protein, said gene being under the transcriptional control of a milk protein promoter which does not naturally control the transcription of said gene, said DNA sequence comprising DNA enabling secretion of said protein, (b) allowing said embryo to develop into an adult mammal, (c) inducing lactation in said mammal, or in a female descendant of said mammal in which said gene, promoter, and signal sequence are present in the mammary tissue genome, (d) collecting milk of said lactating mammal, and (e) isolating said protein from said collected milk.
References Cited (Backward Citations)
EP0169672B1
Cited
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