US5895833A
Expired / Lapsed
US
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Production of human recombinant collagen in the milk of transgenic animals
Assignee: Angiotech Pharmaceuticals Inc
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Filing: 1995-06-07
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Granted: 1999-04-20
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Expires: 2015-06-07
Abstract
Abstract Production of human procollagen or collagen in cells which ordinarily do not produce these molecules is effected by constructing expression systems compatible with mammary glands of non-human mammals. For example, expression systems can be microinjected into fertilized oocytes and reimplanted in foster mothers and carried to term in order to obtain transgenic non-human mammals capable of producing milk containing recombinant human procollagen or collagen. Human procollagen or collagen produced in this manner can be made of a single collagen type uncontaminated by other human or non-human collagens.
Inventors
- 👤 Richard A. Berg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 08:17
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/8509; A; A01; A01K; A01K67/00; A01K67/027; A01K67/0275; A01K67/0278; A23; A23C; A23C9/00; A23C9/20; A23J; A23J1/00; A23J1/20; A23J3/00; A23J3/04; A23J3/06; C07; C07K; C07K14/00; C07K14/435; C07K14/78; A01K2207/00; A01K2207/15; A01K2217/00; A01K2217/05; A01K2227/00; A01K2227/10; A01K2227/105; A01K2267/00; A01K2267/01; A23C2230/00; A23C2230/05; Y; Y10; Y10S; Y10S119/00; Y10S119/01; Y10S530/00; Y10S530/827; Y10S530/832
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
119 words
A method of preparing a single type of trimeric human procollagen, collagen, or a mixture of such procollagen and collagen, which method comprises the step of: recovering said procollagen, collagen, or mixture from milk of a transgenic mammal comprising an expression system comprising a coding nucleotide sequence encoding a human procollagen polypeptide chain operably linked to a control nucleotide sequence that effects expression specifically in milk protein-secreting epithelial cells of a mammary gland in said mammal, and said cells express said coding nucleotide sequence to produce said polypeptide chain, and secrete said procollagen, collagen, or mixture comprising said chain in said milk without extracellular aggregation of said procollagen, collagen or mixture which would prevent excretion from said mammary gland.
Claim 2
Independent Claim
119 words
A method of preparing a single type of trimeric human procollagen, collagen, or a mixture of such procollagen and collagen, which method comprises the step of: recovering said procollagen, collagen, or mixture from milk of a transgenic mammal comprising an expression system comprising a coding nucleotide sequence encoding a human procollagen polypeptide chain operably linked to a control nucleotide sequence that effects expression specifically in milk protein-secreting epithelial cells of a mammary gland in said mammal, and said cells express said coding nucleotide sequence to produce said polypeptide chain, and secrete said procollagen, collagen, or mixture comprising said chain in said milk without extracellular aggregation of said procollagen, collagen or mixture which would prevent excretion from said mammary gland.
Claim 3
Dependent Claim
18 words
The method of claim 1, wherein said control nucleotide sequence comprises a bovine alpha S1-casein promoter DNA sequence.
Claim 4
Dependent Claim
29 words
The method of claim 2, wherein said single type of human procollagen is human Type I procollagen and said single type of human collagen is human Type I collagen.
Claim 5
Dependent Claim
104 words
A transgenic nonhuman mammal whose somatic and germ cells contain an expression system comprising a coding nucleotide sequence encoding a human procollagen polypeptide chain operably linked to a control nucleotide sequence that effects expression specifically in milk protein-secreting epithelial cells of a mammary gland in said mammal, and said cells express said coding nucleotide sequence to produce said polypeptide chain, and secrete a single type of human procollagen, collagen, or a mixture thereof into the milk of said mammal, wherein said milk comprises trimeric collagen, procollagen, or a mixture thereof, in recoverable amounts without extracellular aggregation which would prevent excretion from said mammary gland.
Claim 6
Dependent Claim
104 words
A transgenic nonhuman mammal whose somatic and germ cells contain an expression system comprising a coding nucleotide sequence encoding a human procollagen polypeptide chain operably linked to a control nucleotide sequence that effects expression specifically in milk protein-secreting epithelial cells of a mammary gland in said mammal, and said cells express said coding nucleotide sequence to produce said polypeptide chain, and secrete a single type of human procollagen, collagen, or a mixture thereof into the milk of said mammal, wherein said milk comprises trimeric collagen, procollagen, or a mixture thereof, in recoverable amounts without extracellular aggregation which would prevent excretion from said mammary gland.
Claim 7
Dependent Claim
31 words
The transgenic nonhuman mammal of claim 4, wherein said single type of human procollagen is human Type I procollagen and said single type of human collagen is human Type I collagen.
Claim 8
Dependent Claim
16 words
The transgenic nonhuman mammal of claim 4, wherein said promoter is a bovine alpha S1-casein promoter.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-04-19 | 2002-10-19 | 2003-04-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-04-19 | 2006-10-19 | 2007-04-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-04-19 | 2010-10-19 | 2011-04-20 | $7700.00 | Window Closed / Lapsed |