US6024918A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for attachment of biomolecules to surfaces of medical devices
Assignee: JARO MICHAEL J
•
Filing: 1998-03-13
•
Granted: 2000-02-15
•
Expires: 2018-03-13
Abstract
Abstract A method for making a medical device having a biomolecule immobilized on a substrate surface is provided. The method includes coating the substrate surface with an amino-functional polysiloxane; and contacting the amino-functional polysiloxane coated surface with a biomolecule under conditions effective to immobilize the biomolecule.
Inventors
- 👤 Marc Hendriks
- 👤 Michel Verhoeven
- 👤 Patrick Cahalan
- 👤 Linda Cahalan
- 👤 Edouard Koulik
- 👤 Mirian Gillissen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 05:42
CPC Classifications
A; A61; A61L; A61L31/00; A61L31/08; A61L31/10; A61L27/00; A61L27/28; A61L27/34; A61L27/50; A61L27/54; A61L31/14; A61L31/16; A61L33/00; A61L33/0005; A61L33/0011; A61L33/0029; A61L2300/00; A61L2300/20; A61L2300/23; A61L2300/236; A61L2300/40; A61L2300/42; A61L2300/60; A61L2300/606; A61L2400/00; A61L2400/18
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
43 words
A method for making a medical device having a biomolecule immobilized on a substrate surface, the method comprising: coating the substrate surface with an amino-functional polysiloxane; and contacting the amino-functional polysiloxane coated surface with a biomolecule under conditions effective to immobilize the biomolecule.
Claim 2
Independent Claim
43 words
A method for making a medical device having a biomolecule immobilized on a substrate surface, the method comprising: coating the substrate surface with an amino-functional polysiloxane; and contacting the amino-functional polysiloxane coated surface with a biomolecule under conditions effective to immobilize the biomolecule.
Claim 3
Independent Claim
42 words
The method of claim I wherein the step of coating the substrate surface comprises: coating the substrate surface with an amino-functional polysiloxane in a liquid carrier; removing the liquid carrier; and contacting the amino-functional polysiloxane coating with water to cure the coating.
Claim 4
Dependent Claim
36 words
The method of claim 2 wherein the steps of removing the liquid carrier and contacting the coating with water comprises contacting the substrate surface having the amino-functional polysiloxane in a liquid carrier thereon with moist air.
Claim 5
Dependent Claim
53 words
The method of claim 1 the step of contacting the amino-functional polysiloxane coated surface with a biomolecule under conditions effective to immobilize the biomolecule comprises contacting the amino-functional polysiloxane coated surface with a biomolecule in a liquid carrier at a temperature of at least about 20° C. for at least about 30 seconds.
Claim 6
Dependent Claim
17 words
The method of claim 4 wherein the liquid carrier comprises a periodate in a buffered aqueous solution.
Claim 7
Dependent Claim
13 words
The method of claim 5 wherein the periodate comprises an alkali metal periodate.
Claim 8
Dependent Claim
26 words
The method of claim 5 wherein the biomolecule comprises heparin and the periodate is present in a sufficient amount to form aldehyde groups on the heparin.
Claim 9
Dependent Claim
22 words
The method of claim 7 wherein the buffered aqueous solution has a pH in a range of about 4.5 to about 8.
Claim 10
Independent Claim
63 words
The method of claim I wherein the biomolecule is selected from the group of an antibacterial agent, an antimicrobial agent, an anticoagulant, an antithrombotic agent, a platelet agent, an anti-inflammatory, an enzyme, a catalyst, a hormone, a growth factor, a drugs, a vitamin, an antibody, an antigen, a nucleic acid, a dye, a DNA segment, an RNA segment, a protein, and a peptide.
Claim 11
Dependent Claim
14 words
The method of claim 1 wherein the biomolecule is synthetically derived or naturally occurring.
Claim 12
Dependent Claim
15 words
The method of claim 1 wherein the substrate is a metal, polymer, ceramic, or glass.
Claim 13
Dependent Claim
11 words
The method of claim 1 wherein the surface formed is biocompatible.
Claim 14
Dependent Claim
12 words
The method of claim 1 wherein the surface formed is blood compatible.
Claim 15
Dependent Claim
49 words
The method of claim 13 wherein the substrate to which there is a biomolecule attached through an amino-functional polysiloxane demonstrates at least a 20% reduction in the amount of elastase formed relative to the same substrate without the biomolecule and the polysiloxane attached thereto when contacted with human blood.
Claim 16
Dependent Claim
50 words
The method of claim 13 wherein the substrate to which there is a biomolecule attached through an amino-functional polysiloxane demonstrates at least a 10% reduction in the amount of thrombin-antithrombin complex formed relative to the same substrate without the biomolecule and the polysiloxane attached thereto when contacted with human blood.
Claim 17
Dependent Claim
51 words
The method of claim 13 wherein the substrate to which there is a biomolecule attached through an amino-functional polysiloxane demonstrates at least a 15% reduction in the amount of Platelet Factor 4 formed relative to the same substrate without the biomolecule and the polysiloxane attached thereto when contacted with human blood.
Claim 18
Dependent Claim
51 words
The method of claim 13 wherein the substrate to which there is a biomolecule attached through an amino-functional polysiloxane demonstrates at least a 40% reduction in the amount of terminal complement complex formed relative to the same substrate without the biomolecule and the polysiloxane attached thereto when contacted with human blood.
Claim 19
Dependent Claim
33 words
The method of claim 13 wherein the substrate to which there is a biomolecule attached through an amino-functional polysiloxane has less than about 1% of the surface of the substrate covered by platelets.
Claim 20
Dependent Claim
12 words
The method of claim 1 wherein the medical device is a stent.
Claim 21
Dependent Claim
13 words
The method of claim 1 wherein the medical device is a blood oxygenator.
Claim 22
Independent Claim
58 words
A method for making a medical device having a biomolecule immobilized on a substrate surface, the method comprising: coating the substrate surface with a solution of an amino-functional polysiloxane; drying the amino-functional polysiloxane solution to form a coated surface having amine functionality; contacting the coated surface with the amine functionality with a biomolecule to form a biocompatible surface.
Claim 23
Independent Claim
58 words
A method for making a medical device having a biomolecule immobilized on a substrate surface, the method comprising: coating the substrate surface with a solution of an amino-functional polysiloxane; drying the amino-functional polysiloxane solution to form a coated surface having amine functionality; contacting the coated surface with the amine functionality with a biomolecule to form a biocompatible surface.
Claim 24
Dependent Claim
31 words
The method of claim 21 wherein prior to the step of contacting the coated surface the method includes a step of combining heparin with a periodate to form an aldehyde-functional heparin.
Claim 25
Dependent Claim
21 words
The method of claim 1 wherein the medical device is a blood oxygenator and the substrate being coated includes hollow fibers.
References Cited (Backward Citations)
US3574673A
Cited
US4239729A
Cited
US4600613A
Cited
US4975247A
Cited
US4872867A
Cited
US5013717A
Cited
US5061738A
Cited
US5342693A
Cited
US5053048A
Cited
US5077372A
Cited
US5272012A
Cited
US5039430A
Cited
US5163952A
Cited
US5458616A
Cited
US5541167A
Cited
US5395468A
Cited
US5536582A
Cited
US5403341A
Cited
US5702823A
Cited
US5589120A
Cited
US5643580A
Cited
US5604288A
Cited
US5599576A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-02-14 | 2003-08-16 | 2004-02-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-02-14 | 2007-08-16 | 2008-02-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-02-14 | 2011-08-16 | 2012-02-15 | $7700.00 | Window Closed / Lapsed |