US6004782A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Hyperexpression of bioelastic polypeptides
Assignee: UAB Research Foundation
•
Filing: 1995-10-13
•
Granted: 1999-12-21
•
Expires: 2015-10-13
Abstract
Abstract A method for overexpressing a bioelastic polypeptide in a host cell is provided. A nucleic acid encoding a bioelastic polypeptide having pentapeptide, tetrapeptide, hexapeptide or nonapeptide repeating units is introduced into a host cell. The host cell is grown under conditions that provide for expression of the polypeptide as at least 40% of the total cellular protein of the host cell.
Inventors
- 👤 Henry Daniell
- 👤 David T. McPherson
- 👤 Dan W. Urry
- 👤 Jie Xu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 12:12
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/001
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
130 words
A method for overexpressing a bioelastic polypeptide in a prokaryotic host cell, said method comprising: (a) introducing into said host cell, a vector comprising a promoter operably linked to a nucleic acid encoding a bioelastic polypeptide comprising pentapeptide, tetrapeptide, hexapeptide or nonapeptide repeating units; and (b) growing said host cell to provide for expression of said polypeptide in the inclusion bodies of said host cells, where the volume of said inclusion bodies comprises 40-90% (v/v) of the total cellular volume of said host cell and the amount of inclusion bodies correlates to the amount of polypeptide expressed; with the proviso that when said promoter is an inducible promoter said host cells are grown and said polypeptide is expressed in the absence of an inducer normally associated with said inducible promoter.
Claim 2
Independent Claim
130 words
A method for overexpressing a bioelastic polypeptide in a prokaryotic host cell, said method comprising: (a) introducing into said host cell, a vector comprising a promoter operably linked to a nucleic acid encoding a bioelastic polypeptide comprising pentapeptide, tetrapeptide, hexapeptide or nonapeptide repeating units; and (b) growing said host cell to provide for expression of said polypeptide in the inclusion bodies of said host cells, where the volume of said inclusion bodies comprises 40-90% (v/v) of the total cellular volume of said host cell and the amount of inclusion bodies correlates to the amount of polypeptide expressed; with the proviso that when said promoter is an inducible promoter said host cells are grown and said polypeptide is expressed in the absence of an inducer normally associated with said inducible promoter.
Claim 3
Dependent Claim
13 words
The method of claim 1 where said prokaryotic host cell is E. coli.
Claim 4
Dependent Claim
26 words
The method of claim 1 wherein the volume of said inclusion bodies comprises at least 60% (v/v) of the total cellular volume of said host cell.
Claim 5
Dependent Claim
26 words
The method of claim 1 wherein the volume of said inclusion bodies comprises at least 80% (v/v) of the total cellular volume of said host cell.
Claim 6
Dependent Claim
24 words
The method of claim 1 wherein the volume of said inclusion bodies comprises 90% (v/v) of the total cellular volume of said host cell.
Claim 7
Dependent Claim
12 words
The method of claim 1 wherein said promoter is an inducible promoter.
References Cited (Backward Citations)
US4187852A
Cited
US4132746A
Cited
US4474851A
Cited
US4500700A
Cited
US4589882A
Cited
US4605413A
Cited
US4952496A
Cited
US4693718A
Cited
US5064430A
Cited
US4976734A
Cited
US4870055A
Cited
US5250516A
Cited
US4783523A
Cited
US5255518A
Cited
US4898926A
Cited
US5032271A
Cited
US5085055A
Cited
US5226292A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-12-20 | 2003-06-21 | 2003-12-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-12-20 | 2007-06-21 | 2007-12-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-12-20 | 2011-06-21 | 2011-12-21 | $7700.00 | Window Closed / Lapsed |