US4589882A
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Enzymatically crosslinked bioelastomers
Assignee: UAB RESEARCH FOUNDATION NON-PROFIT CORP
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Filing: 1983-09-19
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Granted: 1986-05-20
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Expires: 2003-09-19
Abstract
Abstract A method of repairing a natural elastic system in a human or animal body, which comprises replacing a damaged portion of the system with a shaped artificial elastomeric copolymer comprising an elastomeric component selected from the group consisting of tetrapeptide and pentapeptide repeating units or mixtures thereof wherein the repeating units comprise amino acid residues selected from the group consisting of hydrophobic amino acid and glycine residues and the repeating units exist in a conformation having a β-turn and a crosslinking component selected from the group consisting of amino acid and peptide residues of the formula ##STR1## wherein α represents a covalent bond or a peptide fragment containing 1-10 α-helix-forming amino acid residues, B represents a covalent bond or a petide fragment containing 1-10 amino acid residues, and n is an integer from 2 to 6; wherein the copolymer optionally comprises a chemotactic component selected from the group consisting of -Ala-Pro-Gly-Val-Gly-Val-, -Pro-Gly-Val-Gly-Val-Ala-, -Gly-Val-Gly-Val-Ala-Pro-, -Val-Gly-Val-Ala-Pro-Gly-, -Gly-Val-Ala-Pro-Gly-Val-, and -Val-Ala-Pro-Gly-Val-Gly- and is essentially devoid of peptide fragments which occur in natural elastin other than these elastomeric, crosslinking, and chemotactic components, is disclosed along with elastomeric copolymers suitable for use in the method of the invention and methods of synthesizing such bioelastomers.
Inventors
- 👤 Dan W. Urry
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 05:42
CPC Classifications
D; D01; D01F; D01F8/00; A; A61; A61L; A61L17/00; A61L27/00; A61L27/14; A61L27/22; A61L27/227; A61L27/40; C; C08; C08G; C08G69/00; C08G69/02; C08G69/08; C08G69/10; C08L; C08L89/00; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/31
Claims Analysis
Total Claims: 31
Claim 1
Independent Claim
113 words
A synthetic elastomeric copolymer, which comprises: an elastomeric component selected, from the group consisting of tetrapeptide repeating units, pentapeptide repeating units and mixtures thereof wherein said repeating units comprise amino acid residues selected from the group consisting of hydrophobic amino acid and glycine residues and said repeating units exist in a conformation having a β-turn, and a crosslinking component selected from the group consisting of amino acid or peptide residues of the formula ##STR9## wherein α represents a covalent bond or a peptide fragment containing 1-10 α-helix-forming amino acid residues, B represents a covalent bond or a peptide fragment containing 1-10 amino acid residues, and n is an integer from 2 to 6.
Claim 2
Dependent Claim
113 words
A synthetic elastomeric copolymer, which comprises: an elastomeric component selected, from the group consisting of tetrapeptide repeating units, pentapeptide repeating units and mixtures thereof wherein said repeating units comprise amino acid residues selected from the group consisting of hydrophobic amino acid and glycine residues and said repeating units exist in a conformation having a β-turn, and a crosslinking component selected from the group consisting of amino acid or peptide residues of the formula ##STR9## wherein α represents a covalent bond or a peptide fragment containing 1-10 α-helix-forming amino acid residues, B represents a covalent bond or a peptide fragment containing 1-10 amino acid residues, and n is an integer from 2 to 6.
Claim 3
Dependent Claim
28 words
The elastomeric copolymer of claim 1, wherein a crosslinking component has the formula ##STR10## wherein A 1 represents a peptide fragment consisting of 2-10 α-helix-forming amino acid residues.
Claim 4
Dependent Claim
51 words
The elastomeric copolymer of claim 2, wherein B is A 2 B 1 wherein A 2 represents a peptide fragment consisting of 2 or 3 α-helix-forming amino acid residues and B 1 represents ##STR11## wherein B 2 represents a covalent bond or a peptide fragment consisting of 1-6 amino acid residues.
Claim 5
Dependent Claim
14 words
The elastomeric copolymer of claim 3, wherein B 2 represents 2-4 amino acid residues.
Claim 6
Dependent Claim
12 words
The elastomeric copolymer of claim 3, wherein B 2 represents -Tyr-Gly-Ala-or -Ala-Ala-.
Claim 7
Dependent Claim
23 words
The elastomeric copolymer of claim 1, wherein said α-helix-forming amino acid residues are alanine, leucine, methionine, glutamine, tryptophan, valine, phenylalanine, or isoleucine residues.
Claim 8
Dependent Claim
15 words
The elastomeric copolymer of claim 1, wherein said α-helix-forming residues are alanine or leucine residues.
Claim 9
Dependent Claim
13 words
The elastomeric copolymer of claim 1, wherein said α-helix-forming residues are alanine residues.
Claim 10
Dependent Claim
10 words
The elastomeric copolymer of claim 1, wherein n is 4.
Claim 11
Dependent Claim
24 words
The elastomeric copolymer of claim 3, wherein said α-helix forming amino acid residues are alanine, leucine, methionine, glutamine, tryptophan, valine, phenylalanine, or isoleucine residues.
Claim 12
Dependent Claim
10 words
The elastomeric copolymer of claim 10, wherein n is 4.
Claim 13
Dependent Claim
38 words
The elastromeric copolymer of claim 11, wherein said crosslinking component comprises the formula -α.sup.1 -α.sup.2 -Lys-α.sup.3 -α.sup.4 -Lys-B.sup.2 - or -α.sup.1 -α.sup.2 -Lys-α.sup.3 -α.sup.4 -α.sup.5 -Lys-B.sup.2 - wherein α 1 -α 5 independently represent α-helix-forming amino acid residues.
Claim 14
Dependent Claim
14 words
The elastomeric copolymer of claim 12, wherein α 1 -α 5 each represents alanine.
Claim 15
Dependent Claim
13 words
The elastomeric copolymer of claim 13, wherein B 2 represents -Tyr-Gly-Ala- or Ala-Ala.
Claim 16
Dependent Claim
22 words
The elastomeric copolymer of claim 1, wherein said hydrophobic amino acid residues are selected from the group consisting of hydrophobic α-amino acids.
Claim 17
Dependent Claim
28 words
The elastomeric copolymer of claim 15, wherein said hydrophobic amino acid residues are selected from the group consisting of alanine, valine, leucine, isoleucine, proline, phenylalanine, tryptophan, and methionine.
Claim 18
Dependent Claim
24 words
The elastomeric copolymer of claim 16, wherein the first amino acid residue of said repeating unit is a residue of valine, leucine, or isoleucine.
Claim 19
Dependent Claim
21 words
The elastomeric copolymer of claim 16, wherein the second amino acid residue of said repeating unit is a residue of proline.
Claim 20
Dependent Claim
43 words
The elastomeric copolymer of claim 16, wherein the third amino acid residue of said repeating unit is a residue of glycine or a hydrophobic amino acid of opposite chirality having no more than 10 carbon atoms in the side chain of said residue.
Claim 21
Dependent Claim
21 words
The elastomeric copolymer of claim 16, wherein the fourth amino acid residue of said repeating unit is a residue of valine.
Claim 22
Dependent Claim
21 words
The elastomeric copolymer of claim 16, wherein the fifth amino acid residue of said repeating unit is a residue of glycine.
Claim 23
Dependent Claim
17 words
The elastomeric copolymer of claim 1, wherein said repeating unit is L-Val-L-Pro-D-Ala-L-Val-Gly, L-Val-L-Pro-Gly-L-Val-Gly, or L-Val-L-Pro-Gly-Gly, or L-Val-L-Pro-D-Ala-Gly.
Claim 24
Dependent Claim
18 words
The elastomeric copolymer of claim 1, wherein the molecules of said elastomeric polypeptide are crosslinked by covalent bonding.
Claim 25
Dependent Claim
18 words
The elastomeric material of claim 23, wherein said molecules are crosslinked by irradiating said polypeptide with ionizing radiation.
Claim 26
Dependent Claim
52 words
The elastomeric material of claim 23, wherein said molecules are crosslinked by reacting a first chemically reactive side group of a modified amino acid residue of a first molecule of said polypeptide with a second chemically reactive side group of a modified amino acid residue of a second molecule of said polypeptide.
Claim 27
Dependent Claim
25 words
An elastomeric composite material, comprising: an artifical core fiber, and the elastomeric material of claim 1 chemically bonded to the surface of said core fiber.
Claim 28
Dependent Claim
22 words
The elastomeric material of claim 26, wherein the weight ratio of said core fiber to said polypeptide is from 10:1 to 1:10.
Claim 29
Dependent Claim
35 words
An elastomeric composite material comprising an essentially non-elastic first artificial fiber, and a second fiber chemically attached to the surface of said first fiber, wherein said second fiber comprises the elastomeric material of claim 1.
Claim 30
Dependent Claim
19 words
A blood vessel prosthesis comprising the material of claim 1 in the form of a patch or hollow tube.
Claim 31
Dependent Claim
27 words
The elastomeric copolymer of claim 1, wherein said copolymer further comprises a chemotactic component selected from the group consisting of -Ala-Pro-Gly-Val-Gly-Val-, -Pro-Gly-Val-Gly-Val-Ala-, -Gly-Val-Gly-Val-Ala-Pro-, -Val-Gly-Val-Ala-Pro-Gly-, -Gly-Val-Ala-Pro-Gly-Val-, and -Val-Ala-Pro-Gly-Val-Gly-.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-05-19 | 1989-11-18 | 1990-05-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-05-19 | 1993-11-18 | 1994-05-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-05-19 | 1997-11-18 | 1998-05-20 | $7700.00 | Window Closed / Lapsed |