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US5965138A Expired / Lapsed US ⏱️ 0.0 years remaining

Recombinant chimeric virus and uses thereof

Assignee: Intervet Inc
•
Filing: 1994-12-22
•
Granted: 1999-10-12
•
Expires: 2014-12-22
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Abstract

Abstract This invention provides a recombinant herpesvirus of turkeys comprising a herpesvirus of turkeys viral genome which contains a foreign DNA sequence inserted within the EcoR1 #9 fragment of the herpesvirus of turkeys viral genome, and the foreign DNA sequence is capable of being expressed in a host cell infected with the herpesvirus of turkeys. This invention provides a recombinant herpesvirus of turkeys-Marek's disease virus chimera comprising a herpesvirus of turkeys unique long viral genome region and a Marek's disease virus unique short region. Lastly, this invention provides homology vectors for producing a recombinant herpesvirus of turkeys, host cells, and vaccines and methods for immunization.

Inventors

  • 👤 Mark D. Cochran
  • 👤 David E. Junker
  • 👤 Martha A. Wild
  • 👤 Phillip A. Singer

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-07 14:43
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16311; C12N2710/16334; C12N2710/16341; C12N2710/16343

Claims Analysis

Total Claims: 22
Claim 1
Independent Claim 34 words
A recombinant chimeric virus comprising a unique long viral genomic region which naturally occurs in a herpesvirus of turkeys and a unique short viral genomic region which naturally occurs in a Marek's disease virus.
Claim 2
Dependent Claim 34 words
A recombinant chimeric virus comprising a unique long viral genomic region which naturally occurs in a herpesvirus of turkeys and a unique short viral genomic region which naturally occurs in a Marek's disease virus.
Claim 3
Dependent Claim 32 words
The recombinant chimeric virus of claim 1, wherein a foreign DNA is inserted within a non essential region of the chimeric virus such that the foreign DNA expressed in a host cell.
Claim 4
Dependent Claim 31 words
The recombinant chimeric virus of claim 2, wherein the non-essential region is an EcoR1 #9 fragment of the unique long viral genomic region which naturally occurs in a herpesvirus of turkeys.
Claim 5
Dependent Claim 14 words
The recombinant chimeric virus of claim 2, wherein the foreign DNA encodes a polypeptide.
Claim 6
Dependent Claim 14 words
The recombinant chimeric virus of claim 2, wherein the foreign DNA encodes a cytokine.
Claim 7
Dependent Claim 22 words
The recombinant chimeric virus of claim 5, wherein the cytokine is a chicken mylemonocytic growth factor (cMGF) or a chicken interferon (cIFN).
Claim 8
Dependent Claim 15 words
The recombinant chimeric virus of claim 2, wherein the foreign DNA encodes E. coli beta-galactosidase.
Claim 9
Dependent Claim 30 words
The recombinant chimeric virus of claim 4, wherein the polypeptide is obtained from Marek's Disease Virus, Newcastle Disease Virus, Infectious Laryngotracheitis Virus, Infectious Bronchitis Virus and Infectious Bursal Disease Virus.
Claim 10
Dependent Claim 27 words
The recombinant chimeric virus of claim 8, wherein the polypeptide is Marek's Disease Virus glycoprotein A, Marek's Disease Virus glycoprotein B, or Marek's Disease Virus glycoprotein D.
Claim 11
Dependent Claim 22 words
The recombinant chimeric virus of claim 8, wherein the polypeptide is a Newcastle Disease virus fusion protein or Newcastle Disease virus hemagglutinin-neuraminidase.
Claim 12
Dependent Claim 27 words
The recombinant chimeric virus of claim 8, wherein the polypeptide is Infectious Laryngotracheitis Virus glycoprotein B, Infectious Laryngotracheitis Virus glycoprotein I, or Infectious Laryngotracheitis Virus glycoprotein D.
Claim 13
Dependent Claim 22 words
The recombinant chimeric virus of claim 8, wherein the polypeptide is Infectious Bronchitis Virus spike protein or Infectious Bronchitis Virus matrix protein.
Claim 14
Dependent Claim 27 words
The recombinant chimeric virus of claim 8, wherein the polypeptide is Infectious Bursal Disease Virus VP2, Infectious Bursal Disease Virus VP3 or Infectious Bursal Disease Virus VP4.
Claim 15
Dependent Claim 19 words
The recombinant chimeric virus of claim 2, wherein the foreign DNA is under the control of a herpesvirus promoter.
Claim 16
Dependent Claim 19 words
The recombinant chimeric virus of claim 2, wherein the foreign DNA is under the control of a heterologous promoter.
Claim 17
Dependent Claim 61 words
The recombinant chimeric virus of claim 14, wherein the promoter is obtained from an endogenous upstream of Pseudorabies Virus glycoprotein X, Herpesvirus Simplex Virus type--1 alpha 4, Human Cytomegalovirus Immediate Early, Marek's Disease Virus glycoprotein A, Marek's Disease Virus glycoprotein B. Marek's Disease Virus glycoprotein D, Infectious Laryngotracheitis Virus glycoprotein B, Bovine Herpesvirus 1.1 VP8, or Infectious Laryngotracheitis virus glycoprotein D.
Claim 18
Dependent Claim 63 words
The recombinant chimeric virus of claim 14, wherein the promoter is obtained from an endogenous upstream sequence of Pseudorabies Virus glycoprotein X, Herpesvirus Simplex Virus type--1 alpha 4, Human Cytomegalovirus Immediate Early, Marek's Disease Virus glycoprotein A, Marek's Disease Virus glycoprotein B, Marek's Disease Virus glycoprotein D, Infectious Laryngotracheitis Virus glycoprotein B, Bovine Herpresvirus 1.1 VP8, or Infectious Laryngotracheitis virus of glycoprotein D.
Claim 19
Dependent Claim 20 words
A vaccine which comprises an effective immunizing amount of the recombinant chimeric virus of claim 1 and a suitable carrier.
Claim 20
Dependent Claim 20 words
A vaccine which comprises an effective immunizing amount of the recombinant chimeric virus of claim 2 and a suitable carrier.
Claim 21
Dependent Claim 20 words
A vaccine which comprises an effective immunizing amount of the recombinant chimeric virus of claim 17 and a suitable carrier.
Claim 22
Dependent Claim 22 words
A method of immunizing a bird which comprises administering to the bird an effecting immunizing dose of the vaccine of claim 18.

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USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 2002-10-11 2003-04-12 2003-10-12 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 2006-10-11 2007-04-12 2007-10-12 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2010-10-11 2011-04-12 2011-10-12 $7700.00 Window Closed / Lapsed

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