Abstract A recombinant Marek's disease is described which comprises a Marek's disease virus genome and a DNA fragment incorporated therein, said DNA fragment containing a promoter derived from an animal cell or an animal virus and a structural gene coding for an exogenous protein. Furthermore a process for preparing said recombinant virus is described which comprises preparing a gene fragment wherein a structural gene coding for an exogenous gene is located downstream of a promoter derived from an animal cell or an animal virus, incorporating said gene fragment into theBamHI - H fragment of a gene of a Marek's disease virus type I, and incorporating said fragment into a Marek's disease virus type I genome. Finally a multivalent live vaccine for birds is described which contains the above recombinant virus.
A process for preparing a recombinant Marek's disease virus which comprises preparing a gene fragment wherein a structural gene coding for an exogenous gene is linked to the downstream of a promoter derived from an animal cell or an animal virus, and incorporating said gene fragment into a BamHI - H region of a Marek's disease virus type I genome.
Claim 2
Dependent Claim
60 words
A process for preparing a recombinant Marek's disease virus which comprises preparing a gene fragment wherein a structural gene coding for an exogenous gene is linked to the downstream of a promoter derived from an animal cell or an animal virus, and incorporating said gene fragment into a BamHI - H region of a Marek's disease virus type I genome.
Claim 3
Dependent Claim
17 words
The process of claim 1 wherein said promoter is a β-actin gene promoter derived from a chicken.
Claim 4
Dependent Claim
47 words
The process of claim 1 wherein the gene fragment wherein the structural gene coding for the exogenous gene is linked to the downstream of the promoter derived from the animal cell or the animal virus is incorporated into a BglII site of the BamHI - H fragment.
Claim 5
Dependent Claim
53 words
A recombinant Marek's disease virus type I which comprises an insertion of heterologous DNA wherein said heterologous DNA is inserted into the BamHI-H fragment of said virus and wherein said heterologous DNA comprises a promoter from an animal cell or animal virus operably linked to a structural gene coding for an exogenous protein.
Claim 6
Dependent Claim
53 words
A recombinant Marek's disease virus type I which comprises an insertion of heterologous DNA wherein said heterologous DNA is inserted into the BamHI-H fragment of said virus and wherein said heterologous DNA comprises a promoter from an animal cell or animal virus operably linked to a structural gene coding for an exogenous protein.
Claim 7
Dependent Claim
20 words
The recombinant Marek's disease virus of claim 4 wherein said promoter is a β-actin gene promoter derived from a chicken.