Patent US3298766A successfully refreshed from Google Patents.
EP0473210A2
Expired / Lapsed
EP
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Recombinant Marek's disease virus
Assignee: Intervet International BV
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Filing: 1991-07-10
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Granted: —
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Expires: 2011-07-10
Abstract
Abstract The present invention relates to the use of Marek's Disease Virus Type I as a viral vector capable of expressing foreign genes in some poultry. Claimed are a nonessential insertion region on the DNA genome of the Virus; a plasmid comprising this region; a host cell containing the plasmid; and a vaccine comprising the recombinant Virus.
Inventors
- 👤 Robin Wilson Morgan
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 08:40
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/63; C12N15/79; C12N15/85; C12N15/86; A; A61; A61P; A61P31/00; A61P31/12; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16311; C12N2710/16341; C12N2710/16343
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
48 words
An insertion region of the DNA genome of Marek's disease virus serotype 1 ("MDV-1") comprising an open reading frame in the terminal 4.3 kb EcoRI-BamHI subfragment of BamHI-A in the unique short region of the genome, having a restriction enzyme site map essentially as illustrated by Figure 1.
Claim 2
Dependent Claim
22 words
An insertion region according to claim 1 comprising the DNA sequence from nucleotide position 238 to 1050 as illustrated in Figure 2.
Claim 3
Dependent Claim
23 words
An insertion region according to claim 2 wherein said region comprises an insertion site containing the recognition sequence for the BglII restriction enzyme.
Claim 4
Dependent Claim
10 words
A plasmid comprising the insertion region according to claim 1.
Claim 5
Dependent Claim
11 words
A plasmid according to claim 4, wherein the plasmid is pMD100.
Claim 6
Dependent Claim
22 words
A plasmid according to claim 4, wherein a foreign gene derived from poultry disease causing agent is inserted into said insertion region.
Claim 7
Dependent Claim
68 words
A plasmid according to claim 6, wherein said foreign gene is selected from the group consisting essentially of Marek's Disease Virus, Infectious Bronchitis Virus, Newcastle Disease Virus, Infectious Bursal Disease Virus, Chicken Anemia Agent, Reovirus, Avian Retrovirus, Fowl Adenovirus, Turkey Rhinotracheitis, Infectious Laryngotracheitis Virus, Eimeria, Salmonella, E. Coli and Mycoplasma gallisepticum, wherein said foreign gene is under control of a promoter capable of driving expression of said sequence.
Claim 8
Dependent Claim
23 words
A plasmid according to claim 6, wherein said foreign gene is under control of a promoter capable of driving expression of said sequence.
Claim 9
Dependent Claim
14 words
A plasmid according to claim 8, wherein the said promoter is SV40 early promoter.
Claim 10
Dependent Claim
10 words
A host cell comprising a plasmid according to claim 4.
Claim 11
Dependent Claim
40 words
A recombinant MDV-1 comprising the genomic DNA of MDV-1 and at least one foreign gene derived from a poultry disease causing agent, wherein said foreign gene is inserted into said genomic DNA in the insertion region according to claim 1.
Claim 12
Dependent Claim
27 words
A recombinant MDV-1 according to claim 11 comprising a promoter for the insertion region, which promoter is functional in a host cell for expressing said foreign gene.
Claim 13
Dependent Claim
26 words
A recombinant MDV-1 according to claim 11, wherein said foreign gene is incorporated in the BglII restriction site of the 4.3 kb EcoRI-BamHI subfragment of BamHI-A.
Claim 14
Dependent Claim
32 words
A recombinant MDV-1 according to claim 12, wherein at least one polypeptide encoded by said foreign gene and at least one polypeptide encoded by said genomic DNA of the MDV-1 is expressed.
Claim 15
Dependent Claim
15 words
A vaccine comprising the polypeptide expressed by the recombinant MDV-1 vector according to claim 12.
Claim 16
Dependent Claim
10 words
A vaccine comprising a recombinant MDV-1 according to claim 12.
Claim 17
Dependent Claim
15 words
A vaccine according to claim 16, comprising at least one polypeptide expressed by said MDV-1.
References Cited (Backward Citations)
No citation records indexed.
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