US5871777A
Expired / Lapsed
US
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Incorporation of biologically active molecules into bioactive glasses
Assignee: University of Pennsylvania
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Filing: 1997-05-02
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Granted: 1999-02-16
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Expires: 2017-05-02
Abstract
Abstract Carriers comprising silica-based glass providing for the controlled release of biologically active molecules, their methods of preparation, and methods of use are disclosed. The carriers are prepared using a sol-gel-derived process. Biologically active molecules are incorporated within the matrix of the glass during production.
Inventors
- 👤 Paul Ducheyne
- 👤 Shulamith Radin
- 👤 Erick Manuel Santos
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:22
CPC Classifications
A; A61; A61K; A61K38/00; A61K38/04; A61K38/14; A61F; A61F2/00; A61F2/02; A61F2/28; A61K38/16; A61K38/17; A61K38/18; A61K38/1841; A61K38/55; A61K38/57; A61K9/00; A61K9/14; A61K9/16; A61K9/1605; A61K9/1611; A61K9/20; A61K9/2004; A61K9/2009; A61L; A61L27/00; A61L27/28; A61L27/30; A61L27/306; A61L27/50; A61L27/54; C; C03; C03C; C03C4/00; C03C4/0007; A61F2/30; A61F2/30767; A61F2002/30001; A61F2002/30667; A61F2002/30677; A61L2300/00; A61L2300/20; A61L2300/252; A61L2300/40; A61L2300/402; A61L2300/404; A61L2300/406; A61L2300/41; A61L2300/412; A61L2300/414; A61L2300/60; A61L2300/602; A61L2430/00; A61L2430/02
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
187 words
A method for preparing a controlled-release carrier comprising silica-based glass having a porous matrix and biologically active molecules incorporated in said matrix comprising the steps of: a) combining a silicon alkoxide precursor with deionized water to form a first mixture; b) adding acid to form a second mixture having a pH in the range of from about 1 to about 4.5; c) adding said biologically active molecules to said second mixture while maintaining the pH within the range of from about 1 to about 4.5 to form a third mixture, said third mixture having a water/precursor molar ratio of from about 6:1 to about 20:1; d) allowing the third mixture to form a gel at a temperature of from about 0° C. to about 40° C.; e) aging the gel at a temperature of from about 0° C. to about 40° C. for from about one day to about four weeks; and f) drying the aged gel at a temperature of from about 15° C. to about 40° C. until a weight loss of from about 50 percent to about 80 percent is observed in said gel.
Claim 2
Independent Claim
187 words
A method for preparing a controlled-release carrier comprising silica-based glass having a porous matrix and biologically active molecules incorporated in said matrix comprising the steps of: a) combining a silicon alkoxide precursor with deionized water to form a first mixture; b) adding acid to form a second mixture having a pH in the range of from about 1 to about 4.5; c) adding said biologically active molecules to said second mixture while maintaining the pH within the range of from about 1 to about 4.5 to form a third mixture, said third mixture having a water/precursor molar ratio of from about 6:1 to about 20:1; d) allowing the third mixture to form a gel at a temperature of from about 0° C. to about 40° C.; e) aging the gel at a temperature of from about 0° C. to about 40° C. for from about one day to about four weeks; and f) drying the aged gel at a temperature of from about 15° C. to about 40° C. until a weight loss of from about 50 percent to about 80 percent is observed in said gel.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein said silicon alkoxide precursor is tetramethylorthosilane.
Claim 4
Dependent Claim
22 words
The method of claim 1 wherein said biologically active molecules comprise from about 0.0001 to about 10% of said carrier by weight.
Claim 5
Dependent Claim
13 words
The method of claim 1 wherein said biologically active molecules comprise a drug.
Claim 6
Dependent Claim
11 words
The method of claim 4 wherein said drug comprises an antibiotic.
Claim 7
Dependent Claim
10 words
The method of claim 5 wherein said antibiotic is vancomycin.
Claim 8
Dependent Claim
13 words
The method of claim 1 wherein said biologically active molecule comprises a protein.
Claim 9
Dependent Claim
12 words
The method of claim 4 wherein said drug is a growth factor.
Claim 10
Dependent Claim
11 words
The method of claim 8 wherein said growth factor is TGF-β.
Claim 11
Dependent Claim
12 words
The method of claim 4 wherein said drug is an anti-inflammatory agent.
Claim 12
Dependent Claim
11 words
The method of claim 4 wherein said drug is an analgesic.
Claim 13
Independent Claim
198 words
A method for preparing a controlled-release carrier comprising silica-based glass having a porous matrix and biologically active molecules incorporated in said matrix comprising the steps of: a) combining a silicon alkoxide precursor with deionized water and methanol in a molar ratio of methanol/precursor of about 1:1 to form a first mixture; b) adding acid to form a second mixture having a pH in the range of from about 1 to about 4.5; c) adding said biologically active molecules to said second mixture while maintaining the pH within the range of from about 1 to about 4.5 to form a third mixture, said third mixture having a water/precursor molar ratio of from about 6:1 to about 20:1; d) allowing the third mixture to form a gel at a temperature of from about 0° C. to about 40° C.; e) aging the gel at a temperature of from about 0° C. to about 40° C. for from about one day to about four weeks; and f) drying the aged gel at a temperature of from about 15° C. to about 40° C. until a weight loss of from about 50 percent to about 80 percent is observed in said gel.
Claim 14
Independent Claim
198 words
A method for preparing a controlled-release carrier comprising silica-based glass having a porous matrix and biologically active molecules incorporated in said matrix comprising the steps of: a) combining a silicon alkoxide precursor with deionized water and methanol in a molar ratio of methanol/precursor of about 1:1 to form a first mixture; b) adding acid to form a second mixture having a pH in the range of from about 1 to about 4.5; c) adding said biologically active molecules to said second mixture while maintaining the pH within the range of from about 1 to about 4.5 to form a third mixture, said third mixture having a water/precursor molar ratio of from about 6:1 to about 20:1; d) allowing the third mixture to form a gel at a temperature of from about 0° C. to about 40° C.; e) aging the gel at a temperature of from about 0° C. to about 40° C. for from about one day to about four weeks; and f) drying the aged gel at a temperature of from about 15° C. to about 40° C. until a weight loss of from about 50 percent to about 80 percent is observed in said gel.
Claim 15
Dependent Claim
12 words
The method of claim 12 wherein said silicon alkoxide precursor is tetramethylorthosilane.
Claim 16
Dependent Claim
22 words
The method of claim 12 wherein said biologically active molecules comprise from about 0.0001 to about 10% of said carrier by weight.
Claim 17
Dependent Claim
13 words
The method of claim 12 wherein said biologically active molecules comprise a drug.
Claim 18
Dependent Claim
11 words
The method of claim 15 wherein said drug comprises an antibiotic.
Claim 19
Dependent Claim
10 words
The method of claim 15 wherein said antibiotic is vancomycin.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-02-15 | 2002-08-17 | 2003-02-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-02-15 | 2006-08-17 | 2007-02-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-02-15 | 2010-08-17 | 2011-02-16 | $7700.00 | Window Closed / Lapsed |