US5858801A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Patterning antibodies on a surface
Assignee: United States Department of the Navy
•
Filing: 1997-03-13
•
Granted: 1999-01-12
•
Expires: 2017-03-13
Abstract
Abstract A patterned multiple antibody substrate for use in biosensors or immunosensors is produced by (1) coating an antibody-adsorbent substrate with a material that resists antibody adsorption, (2) using ion beam sputtering, laser ablation, or mechanical scribing to remove the coating at specific sites on the substrate, and then (3) adsorbing specific antibodies at the sites. The substrate is capable of detecting multiple chemical species simultaneously.
Inventors
- 👤 Robert A. Brizzolara
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 09:12
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; Y; Y10; Y10S; Y10S435/00; Y10S435/973; Y10S977/00; Y10S977/84; Y10S977/849; Y10S977/855; Y10S977/856
Claims Analysis
Total Claims: 80
Claim 1
Independent Claim
127 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to adsorption of antibodies; B. removing a portion of the antibody-resistant material to produce a bare site on the antibody-adsorbent substrate having a precise size, shape, and location on the substrate; C. adsorbing molecules of a selected antibody on to the bare site on the antibody adsorbent-substrate; D. rinsing the substrate to remove unadsorbed antibody molecules; E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the newly adsorbed antibody molecules; and F. repeating steps B through E until each of the antibodies has been adsorbed at its specific site on the antibody adsorbent substrate.
Claim 2
Dependent Claim
127 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to adsorption of antibodies; B. removing a portion of the antibody-resistant material to produce a bare site on the antibody-adsorbent substrate having a precise size, shape, and location on the substrate; C. adsorbing molecules of a selected antibody on to the bare site on the antibody adsorbent-substrate; D. rinsing the substrate to remove unadsorbed antibody molecules; E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the newly adsorbed antibody molecules; and F. repeating steps B through E until each of the antibodies has been adsorbed at its specific site on the antibody adsorbent substrate.
Claim 3
Dependent Claim
15 words
The process of claim 1 wherein the antibody-adsorbent substrate is composed of a hydrophobic material.
Claim 4
Dependent Claim
16 words
The process of claim 2 wherein the antibody-adsorbent substrate is composed of a hydrophobic, polymeric material.
Claim 5
Dependent Claim
21 words
The process of claim 3 wherein the antibody-adsorbent substrate is composed of polystyrene, polypropylene, polyethylene, or copolymers of polypropylene and polyethylene.
Claim 6
Dependent Claim
15 words
The process of claim 4 wherein the antibody-adsorbent substrate is composed of polystyrene or polypropylene.
Claim 7
Dependent Claim
26 words
The process of claim 1 wherein the antibody-resistant material is bovine serum albumin, gelatin, lysozyme, octoxynol, polyoxyethylenesorbitan monolaurate, or a polyethylene oxide containing block copolymer surfactant.
Claim 8
Dependent Claim
16 words
The process of claim 6 wherein the antibody-resistant material is bovine serum albumin, gelatin, or lysozyme.
Claim 9
Dependent Claim
13 words
The process of claim 7 wherein the antibody-resistant material is bovine serum albumin.
Claim 10
Dependent Claim
108 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to the adsorption of antibodies; B. removing portions of the antibody-resistant material to produce bare sites on the antibody-adsorbent substrate having precise sizes and shapes and each site having a precise location which corresponds to a specific antibody; C. adsorbing molecules of each antibody to its specific bare site on the antibody-adsorbent substrate; D. rinsing the substrate to remove unadsorbed antibodies; and E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the adsorbed antibody molecules.
Claim 11
Dependent Claim
108 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to the adsorption of antibodies; B. removing portions of the antibody-resistant material to produce bare sites on the antibody-adsorbent substrate having precise sizes and shapes and each site having a precise location which corresponds to a specific antibody; C. adsorbing molecules of each antibody to its specific bare site on the antibody-adsorbent substrate; D. rinsing the substrate to remove unadsorbed antibodies; and E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the adsorbed antibody molecules.
Claim 12
Dependent Claim
25 words
The process of claim 9 wherein each antibody adsorbed in step C is delivered to its precise bare site on the antibody-adsorbent substrate by micropipetting.
Claim 13
Dependent Claim
28 words
The process of claim 9 wherein each antibody adsorbed in step C is delivered to its precise bare site on the antibody-absorbent substrate by an ink jet printer.
Claim 14
Dependent Claim
15 words
The process of claim 9 wherein the antibody-adsorbent substrate is composed of a hydrophobic material.
Claim 15
Dependent Claim
16 words
The process of claim 12 wherein the antibody-adsorbent substrate is composed of a hydrophobic, polymeric material.
Claim 16
Dependent Claim
22 words
The process of claim 13 wherein the antibody adsorbent substrate is composed of polystyrene, polypropylene, polyethylene, or copolymers of polypropylene and polyethylene.
Claim 17
Dependent Claim
15 words
The process of claim 14 wherein the antibody-adsorbent substrate is composed of polystyrene or polypropylene.
Claim 18
Dependent Claim
26 words
The process of claim 9 wherein the antibody-resistant material is bovine serum albumin, gelatin, lysozyme, octoxynol, polyoxyethylenesorbitan monolaurate, or a polyethylene oxide containing block copolymer surfactant.
Claim 19
Dependent Claim
16 words
The process of claim 16 wherein the antibody-resistant material is bovine serum albumin, gelatin, or lysozyme.
Claim 20
Dependent Claim
13 words
The process of claim 17 wherein the antibody-resistant material is bovine serum albumin.
Claim 21
Dependent Claim
131 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to adsorption of antibodies; B. removing a portion of the antibody-resistant material by ion beam sputtering to produce a bare site on the antibody-adsorbent substrate having a precise size, shape, and location on the substrate; C. adsorbing molecules of a selected antibody on to the bare site on the antibody adsorbent-substrate; D. rinsing the substrate to remove unadsorbed antibody molecules; E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the newly adsorbed antibody molecules; and F. repeating steps B through E until each of the antibodies has been adsorbed at its specific site on the antibody adsorbent substrate.
Claim 22
Dependent Claim
131 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to adsorption of antibodies; B. removing a portion of the antibody-resistant material by ion beam sputtering to produce a bare site on the antibody-adsorbent substrate having a precise size, shape, and location on the substrate; C. adsorbing molecules of a selected antibody on to the bare site on the antibody adsorbent-substrate; D. rinsing the substrate to remove unadsorbed antibody molecules; E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the newly adsorbed antibody molecules; and F. repeating steps B through E until each of the antibodies has been adsorbed at its specific site on the antibody adsorbent substrate.
Claim 23
Dependent Claim
15 words
The process of claim 19 wherein the antibody-adsorbent substrate is composed of a hydrophobic material.
Claim 24
Dependent Claim
16 words
The process of claim 20 wherein the antibody-adsorbent substrate is composed of a hydrophobic, polymeric material.
Claim 25
Dependent Claim
21 words
The process of claim 21 wherein the antibody-adsorbent substrate is composed of polystyrene, polypropylene, polyethylene, or copolymers of polypropylene and polyethylene.
Claim 26
Dependent Claim
15 words
The process of claim 22 wherein the antibody-adsorbent substrate is composed of polystyrene or polypropylene.
Claim 27
Dependent Claim
26 words
The process of claim 19 wherein the antibody-resistant material is bovine serum albumin, gelatin, lysozyme, octoxynol, polyoxyethylenesorbitan monolaurate, or a polyethylene oxide containing block copolymer surfactant.
Claim 28
Dependent Claim
16 words
The process of claim 24 wherein the antibody-resistant material is bovine serum albumin, gelatin, or lysozyme.
Claim 29
Dependent Claim
13 words
The process of claim 25 wherein the antibody-resistant material is bovine serum albumin.
Claim 30
Dependent Claim
130 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to adsorption of antibodies; B. removing a portion of the antibody-resistant material by laser ablation to produce a bare site on the antibody-adsorbent substrate having a precise size, shape, and location on the substrate; C. adsorbing molecules of a selected antibody on to the bare site on the antibody adsorbent-substrate; D. rinsing the substrate to remove unadsorbed antibody molecules; E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the newly adsorbed antibody molecules; and F. repeating steps B through E until each of the antibodies has been adsorbed at its specific site on the antibody adsorbent substrate.
Claim 31
Dependent Claim
130 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to adsorption of antibodies; B. removing a portion of the antibody-resistant material by laser ablation to produce a bare site on the antibody-adsorbent substrate having a precise size, shape, and location on the substrate; C. adsorbing molecules of a selected antibody on to the bare site on the antibody adsorbent-substrate; D. rinsing the substrate to remove unadsorbed antibody molecules; E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the newly adsorbed antibody molecules; and F. repeating steps B through E until each of the antibodies has been adsorbed at its specific site on the antibody adsorbent substrate.
Claim 32
Dependent Claim
15 words
The process of claim 27 wherein the antibody-adsorbent substrate is composed of a hydrophobic material.
Claim 33
Dependent Claim
16 words
The process of claim 28 wherein the antibody-adsorbent substrate is composed of a hydrophobic, polymeric material.
Claim 34
Dependent Claim
21 words
The process of claim 29 wherein the antibody-adsorbent substrate is composed of polystyrene, polypropylene, polyethylene, or copolymers of polypropylene and polyethylene.
Claim 35
Dependent Claim
15 words
The process of claim 30 wherein the antibody-adsorbent substrate is composed of polystyrene or polypropylene.
Claim 36
Dependent Claim
26 words
The process of claim 27 wherein the antibody-resistant material is bovine serum albumin, gelatin, lysozyme, octoxynol, polyoxyethylenesorbitan monolaurate, or a polyethylene oxide containing block copolymer surfactant.
Claim 37
Dependent Claim
16 words
The process of claim 32 wherein the antibody-resistant material is bovine serum albumin, gelatin, or lysozyme.
Claim 38
Dependent Claim
13 words
The process of claim 33 wherein the antibody-resistant material is bovine serum albumin.
Claim 39
Dependent Claim
135 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to adsorption of antibodies; B. removing a portion of the antibody-resistant material by laser mechanical scribing using atomic force microscopy to produce a bare site on the antibody-adsorbent substrate having a precise size, shape, and location on the substrate; C. adsorbing molecules of a selected antibody on to the bare site on the antibody adsorbent-substrate; D. rinsing the substrate to remove unadsorbed antibody molecules; E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the newly adsorbed antibody molecules; and F. repeating steps B through E until each of the antibodies has been adsorbed at its specific site on the antibody adsorbent substrate.
Claim 40
Dependent Claim
135 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to adsorption of antibodies; B. removing a portion of the antibody-resistant material by laser mechanical scribing using atomic force microscopy to produce a bare site on the antibody-adsorbent substrate having a precise size, shape, and location on the substrate; C. adsorbing molecules of a selected antibody on to the bare site on the antibody adsorbent-substrate; D. rinsing the substrate to remove unadsorbed antibody molecules; E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the newly adsorbed antibody molecules; and F. repeating steps B through E until each of the antibodies has been adsorbed at its specific site on the antibody adsorbent substrate.
Claim 41
Dependent Claim
15 words
The process of claim 35 wherein the antibody-adsorbent substrate is composed of a hydrophobic material.
Claim 42
Dependent Claim
16 words
The process of claim 36 wherein the antibody-adsorbent substrate is composed of a hydrophobic, polymeric material.
Claim 43
Dependent Claim
21 words
The process of claim 37 wherein the antibody-adsorbent substrate is composed of polystyrene, polypropylene, polyethylene, or copolymers of polypropylene and polyethylene.
Claim 44
Dependent Claim
15 words
The process of claim 38 wherein the antibody-adsorbent substrate is composed of polystyrene or polypropylene.
Claim 45
Dependent Claim
26 words
The process of claim 35 wherein the antibody-resistant material is bovine serum albumin, gelatin, lysozyme, octoxynol, polyoxyethylenesorbitan monolaurate, or a polyethylene oxide containing block copolymer surfactant.
Claim 46
Dependent Claim
16 words
The process of claim 40 wherein the antibody-resistant material is bovine serum albumin, gelatin, or lysozyme.
Claim 47
Dependent Claim
13 words
The process of claim 41 wherein the antibody-resistant material is bovine serum albumin.
Claim 48
Dependent Claim
112 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to the adsorption of antibodies; B. removing portions of the antibody-resistant material by ion beam sputtering to produce bare sites on the antibody-adsorbent substrate having precise sizes and shapes and each site having a precise location which corresponds to a specific antibody; C. adsorbing molecules of each antibody to its specific bare site on the antibody-adsorbent substrate; D. rinsing the substrate to remove unadsorbed antibodies; and E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the adsorbed antibody molecules.
Claim 49
Dependent Claim
112 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to the adsorption of antibodies; B. removing portions of the antibody-resistant material by ion beam sputtering to produce bare sites on the antibody-adsorbent substrate having precise sizes and shapes and each site having a precise location which corresponds to a specific antibody; C. adsorbing molecules of each antibody to its specific bare site on the antibody-adsorbent substrate; D. rinsing the substrate to remove unadsorbed antibodies; and E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the adsorbed antibody molecules.
Claim 50
Dependent Claim
25 words
The process of claim 43 wherein each antibody adsorbed in step C is delivered to its precise bare site on the antibody-adsorbent substrate by micropipetting.
Claim 51
Dependent Claim
28 words
The process of claim 43 wherein each antibody adsorbed in step C is delivered to its precise bare site on the antibody-absorbent substrate by an ink jet printer.
Claim 52
Dependent Claim
15 words
The process of claim 43 wherein the antibody-adsorbent substrate is composed of a hydrophobic material.
Claim 53
Dependent Claim
16 words
The process of claim 46 wherein the antibody-adsorbent substrate is composed of a hydrophobic, polymeric material.
Claim 54
Dependent Claim
22 words
The process of claim 47 wherein the antibody adsorbent substrate is composed of polystyrene, polypropylene, polyethylene, or copolymers of polypropylene and polyethylene.
Claim 55
Dependent Claim
15 words
The process of claim 48 wherein the antibody-adsorbent substrate is composed of polystyrene or polypropylene.
Claim 56
Dependent Claim
26 words
The process of claim 43 wherein the antibody-resistant material is bovine serum albumin, gelatin, lysozyme, octoxynol, polyoxyethylenesorbitan monolaurate, or a polyethylene oxide containing block copolymer surfactant.
Claim 57
Dependent Claim
16 words
The process of claim 50 wherein the antibody-resistant material is bovine serum albumin, gelatin, or lysozyme.
Claim 58
Dependent Claim
13 words
The process of claim 51 wherein the antibody-resistant material is bovine serum albumin.
Claim 59
Dependent Claim
111 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to the adsorption of antibodies; B. removing portions of the antibody-resistant material by laser ablation to produce bare sites on the antibody-adsorbent substrate having precise sizes and shapes and each site having a precise location which corresponds to a specific antibody; C. adsorbing molecules of each antibody to its specific bare site on the antibody-adsorbent substrate; D. rinsing the substrate to remove unadsorbed antibodies; and E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the adsorbed antibody molecules.
Claim 60
Dependent Claim
111 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to the adsorption of antibodies; B. removing portions of the antibody-resistant material by laser ablation to produce bare sites on the antibody-adsorbent substrate having precise sizes and shapes and each site having a precise location which corresponds to a specific antibody; C. adsorbing molecules of each antibody to its specific bare site on the antibody-adsorbent substrate; D. rinsing the substrate to remove unadsorbed antibodies; and E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the adsorbed antibody molecules.
Claim 61
Dependent Claim
25 words
The process of claim 53 wherein each antibody adsorbed in step C is delivered to its precise bare site on the antibody-adsorbent substrate by micropipetting.
Claim 62
Dependent Claim
28 words
The process of claim 53 wherein each antibody adsorbed in step C is delivered to its precise bare site on the antibody-absorbent substrate by an ink jet printer.
Claim 63
Dependent Claim
15 words
The process of claim 53 wherein the antibody-adsorbent substrate is composed of a hydrophobic material.
Claim 64
Dependent Claim
16 words
The process of claim 56 wherein the antibody-adsorbent substrate is composed of a hydrophobic, polymeric material.
Claim 65
Dependent Claim
22 words
The process of claim 57 wherein the antibody adsorbent substrate is composed of polystyrene, polypropylene, polyethylene, or copolymers of polypropylene and polyethylene.
Claim 66
Dependent Claim
15 words
The process of claim 58 wherein the antibody-adsorbent substrate is composed of polystyrene or polypropylene.
Claim 67
Dependent Claim
26 words
The process of claim 53 wherein the antibody-resistant material is bovine serum albumin, gelatin, lysozyme, octoxynol, polyoxyethylenesorbitan monolaurate, or a polyethylene oxide containing block copolymer surfactant.
Claim 68
Dependent Claim
16 words
The process of claim 60 wherein the antibody-resistant material is bovine serum albumin, gelatin, or lysozyme.
Claim 69
Dependent Claim
13 words
The process of claim 61 wherein the antibody-resistant material is bovine serum albumin.
Claim 70
Dependent Claim
115 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to the adsorption of antibodies; B. removing portions of the antibody-resistant material by mechanical scribing using atomic force microscopy to produce bare sites on the antibody-adsorbent substrate having precise sizes and shapes and each site having a precise location which corresponds to a specific antibody; C. adsorbing molecules of each antibody to its specific bare site on the antibody-adsorbent substrate; D. rinsing the substrate to remove unadsorbed antibodies; and E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the adsorbed antibody molecules.
Claim 71
Dependent Claim
115 words
A process for producing a multiple antibody patterned substrate comprising: A. coating an antibody-adsorbent substrate with an antibody-resistant material that is resistant to the adsorption of antibodies; B. removing portions of the antibody-resistant material by mechanical scribing using atomic force microscopy to produce bare sites on the antibody-adsorbent substrate having precise sizes and shapes and each site having a precise location which corresponds to a specific antibody; C. adsorbing molecules of each antibody to its specific bare site on the antibody-adsorbent substrate; D. rinsing the substrate to remove unadsorbed antibodies; and E. coating the antibody-adsorbent substrate with more of the antibody-resistant material to cover the bare surface of the substrate between the adsorbed antibody molecules.
Claim 72
Dependent Claim
25 words
The process of claim 63 wherein each antibody adsorbed in step C is delivered to its precise bare site on the antibody-adsorbent substrate by micropipetting.
Claim 73
Dependent Claim
28 words
The process of claim 63 wherein each antibody adsorbed in step C is delivered to its precise bare site on the antibody-absorbent substrate by an ink jet printer.
Claim 74
Dependent Claim
15 words
The process of claim 63 wherein the antibody-adsorbent substrate is composed of a hydrophobic material.
Claim 75
Dependent Claim
16 words
The process of claim 66 wherein the antibody-adsorbent substrate is composed of a hydrophobic, polymeric material.
Claim 76
Dependent Claim
22 words
The process of claim 67 wherein the antibody adsorbent substrate is composed of polystyrene, polypropylene, polyethylene, or copolymers of polypropylene and polyethylene.
Claim 77
Dependent Claim
15 words
The process of claim 68 wherein the antibody-adsorbent substrate is composed of polystyrene or polypropylene.
Claim 78
Dependent Claim
26 words
The process of claim 63 wherein the antibody-resistant material is bovine serum albumin, gelatin, lysozyme, octoxynol, polyoxyethylenesorbitan monolaurate, or a polyethylene oxide containing block copolymer surfactant.
Claim 79
Dependent Claim
16 words
The process of claim 70 wherein the antibody-resistant material is bovine serum albumin, gelatin, or lysozyme.
Claim 80
Dependent Claim
13 words
The process of claim 71 wherein the antibody-resistant material is bovine serum albumin.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2002-01-11 | 2002-07-13 | 2003-01-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-01-11 | 2006-07-13 | 2007-01-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-01-11 | 2010-07-13 | 2011-01-12 | $7700.00 | Window Closed / Lapsed |