US4011350A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of making microscope slide system
Assignee: Clinical Sciences Inc
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Filing: 1973-05-29
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Granted: 1977-03-08
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Expires: 1993-05-29
Abstract
Abstract A microscope slide assembly and a method of preparing such which includes an optically clear glass slide having a controlled thickness fluorocarbon layer coated on one planar surface. The layer is provided with a multiplicity of openings to form wells on the glass slide. An agar solution may be incorporated within the wells to provide an adhesive layer. In addition, a particular antigen may be layered on top of the agar composition to provide a completed assembly wherein only a patient's serum is needed to process a microscopic examination. For use in fluorescent microscopy, a film body grade Teflon containing an adhesive is used to provide the uniform fluorocarbon layer. The microscopic assembly may be enclosed within an environment isolation container to prevent contamination of the assembly from the ambient surroundings when the antigen is combined with the agar solution.
Inventors
- 👤 Arthur L. Markovits
- 👤 Stanley W. Wolfson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 09:06
CPC Classifications
G; G01; G01N; G01N1/00; G01N1/28; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/548; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/807; Y10S436/808; Y10S436/809; Y10S436/825
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
90 words
A method of preparing a microscope slide system including the steps of: a. spraying a layer of fluorocarbon on one surface of a microscope slide, said layer having an approximate thickness range between 7.5 × 10 - 5 and 1.5 × 10 - 4 inch, said fluorocarbon layer having a plurality of openings passing therethrough for forming reactant regions on said surface of said microscope slide; and, b. applying a layer of 0.02% agarose solution to said surface of said microscope slide only within and completely covering said reactant region.
Claim 2
Independent Claim
90 words
A method of preparing a microscope slide system including the steps of: a. spraying a layer of fluorocarbon on one surface of a microscope slide, said layer having an approximate thickness range between 7.5 × 10 - 5 and 1.5 × 10 - 4 inch, said fluorocarbon layer having a plurality of openings passing therethrough for forming reactant regions on said surface of said microscope slide; and, b. applying a layer of 0.02% agarose solution to said surface of said microscope slide only within and completely covering said reactant region.
Claim 3
Dependent Claim
74 words
The method of preparing a microscope slide system as recited in claim 1 wherein the step of applying said layer of agarose includes the steps of: a. forming an agarose solution with distilled water; b. maintaining said agarose solution within a temperature range of approximately 55° to 60° C; c. inserting a predetermined quantity of solution within said reactant regions of said microscope slide; and, d. drying said agarose solution within said reactant regions.
Claim 4
Dependent Claim
79 words
The method of preparing a microscopic slide system as recited in claim 2 wherein the step of forming an agarose solution includes the steps of: a. mixing a predetermined quantity of agarose with a predetermined amount of distilled water within a flask to form an agarose suspension; b. warming said suspension to a temperature approximating 100° C. to form an agarose solution; and c. tempering said warmed agarose solution until a temperature between 55° and 60° C. is reached.
Claim 5
Dependent Claim
39 words
The method of preparing a microscopic slide system as recited in claim 3 wherein the step of warming said agarose suspension includes the step of placing said suspension containing flask into boiling water until said suspension forms a solution.
Claim 6
Dependent Claim
44 words
The method of preparing a microscope slide system as recited in claim 2 wherein the step of inserting said agarose solution into said reactant regions includes the step of dropping one one hundred twentieth mm. of agarose solution into each of said reactant regions.
Claim 7
Dependent Claim
36 words
The method of preparing a microscope slide system as recited in claim 1 including the additional step of adding a predetermined quantity of a specific antigen within said reactant regions after application of said agarose solution.
Claim 8
Dependent Claim
79 words
The method of preparing a microscope slide system as recited in claim 6 wherein the step of adding antigen includes the steps of: a. removing surface proteins and other interfering debris from said antigen; b. adjusting said antigen in buffering solution to provide a predetermined concentration of antigen cells; c. inserting said antigen into said reactant regions in a substantially uniform thickness layer; and, d. placing said antigen coated slide into a fixative for a predetermined length of time.
Claim 9
Dependent Claim
43 words
The method of preparing a microscope slide system as recited in claim 7 wherein the step of adjusting said antigen includes the step of establishing a concentration of 15 to 30 observable antigen cells within each of said reactant regions of said slide.
Claim 10
Dependent Claim
65 words
The method of preparing a microscope slide system as recited in claim 8 wherein the step of inserting said antigen includes the steps of: a. placing a one lambda volume of each of said antigens into each of said reactant regions; and, b. drying said layer of antigens placed within said reactant regions, said antigen layers being contiguous with said 0.02% solution of dried agarose.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-03-07 | 1980-09-06 | 1981-03-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-03-07 | 1984-09-06 | 1985-03-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-03-07 | 1988-09-06 | 1989-03-08 | $7700.00 | Window Closed / Lapsed |