US5744305A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Arrays of materials attached to a substrate
Assignee: Affymetrix Inc
•
Filing: 1995-06-06
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Granted: 1998-04-28
•
Expires: 2015-06-06
Abstract
Abstract A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed spatially-addressable parallel chemical synthesis. Binary masking techniques are utilized in one embodiment. A reactor system, photoremovable protective groups, and improved data collection and handling techniques are also disclosed. A technique for screening linker molecules is also provided.
Inventors
- 👤 Stephen P.A. Fodor
- 👤 Lubert Stryer
- 👤 J. Leighton Read
- 👤 Michael C. Pirrung
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 18:43
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/645; G01N21/6452; B; B01; B01J; B01J19/00; B01J19/0046; B82; B82Y; B82Y10/00; B82Y30/00; C; C07; C07C; C07C229/00; C07C229/02; C07C229/04; C07C229/06; C07C229/10; C07C229/14; C07C229/16; C07D; C07D263/00; C07D263/02; C07D263/30; C07D263/34; C07D263/44; C07D317/00; C07D317/08; C07D317/44; C07D317/46; C07D317/48; C07D317/62; C07H; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/073; C07H19/16; C07H19/173; C07H21/00; C07H21/04; C07K; C07K1/00; C07K1/04; C07K1/042; C07K1/045; C07K1/047; C07K1/06; C07K1/061; C07K1/062; C07K17/00; C07K17/02; C07K17/06; C07K17/14; C07K7/00; C07K7/04; C07K7/06; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6809; C12Q1/6813; C12Q1/6816; C12Q1/6834; C12Q1/6837; C12Q1/6869; C12Q1/6874; C40; C40B; C40B80/00; G01N21/17; G01N21/25; G01N21/251; G01N21/253; G01N21/6428; G03; G03F; G03F7/00; G03F7/004; G03F7/0045; G03F7/26; G03F7/265; G03F7/38; G11; G11C; G11C13/00; G11C13/0002; G11C13/0009; G11C13/0014; G11C13/0019; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00306; B01J2219/00313; B01J2219/00315; B01J2219/00351; B01J2219/00427; B01J2219/00432; B01J2219/00434; B01J2219/00436; B01J2219/00457; B01J2219/00459; B01J2219/00468; B01J2219/00475; B01J2219/00497; B01J2219/005; B01J2219/00527; B01J2219/00529; B01J2219/00531; B01J2219/00583; B01J2219/00585; B01J2219/0059; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/00608; B01J2219/00612; B01J2219/00614; B01J2219/00617; B01J2219/00623; B01J2219/00626; B01J2219/00632; B01J2219/00637; B01J2219/00646; B01J2219/00648; B01J2219/00659; B01J2219/0068; B01J2219/00686; B01J2219/00689; B01J2219/00695; B01J2219/00709; B01J2219/00711; B01J2219/00718; B01J2219/0072; B01J2219/00722; B01J2219/00725; C07B; C07B2200/00; C07B2200/11; C40B40/00; C40B40/04; C40B40/06; C40B40/10; C40B60/00; C40B60/14; G01N15/00; G01N15/10; G01N15/14; G01N15/1429; G01N15/1433; G01N21/6456; G01N21/6458; Y; Y02; Y02P; Y02P20/00; Y02P20/50; Y02P20/55; Y10; Y10S; Y10S435/00; Y10S435/969; Y10S435/973; Y10S436/00; Y10S436/807; Y10S436/809
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
75 words
An array of oligonucleotides, the array comprising: a planar, non-porous solid support having at least a first surface; and a plurality of different oligonucleotides attached to the first surface of the solid support at a density exceeding 400 different oligonucleotides/cm 2 , wherein each of the different oligonucleotides is attached to the surface of the solid support in a different predefined region, has a different determinable sequence, and is at least 4 nucleotides in length.
Claim 2
Dependent Claim
75 words
An array of oligonucleotides, the array comprising: a planar, non-porous solid support having at least a first surface; and a plurality of different oligonucleotides attached to the first surface of the solid support at a density exceeding 400 different oligonucleotides/cm 2 , wherein each of the different oligonucleotides is attached to the surface of the solid support in a different predefined region, has a different determinable sequence, and is at least 4 nucleotides in length.
Claim 3
Dependent Claim
19 words
The array of claim 1, wherein each different oligonucleotides is from about 4 to about 20 nucleotides in length.
Claim 4
Dependent Claim
16 words
The array of claim 1, wherein each different oligonucleotide is at least 10 nucleotides in length.
Claim 5
Dependent Claim
16 words
The array of claim 1, wherein each different oligonucleotide is at least 20 nucleotides in length.
Claim 6
Dependent Claim
23 words
The array of claim 1, wherein the array comprises at least 1,000 different oligonucleotides attached to the first surface of the solid support.
Claim 7
Dependent Claim
23 words
The array of claim 1, wherein the array comprises at least 10,000 different oligonucleotides attached to the first surface of the solid support.
Claim 8
Dependent Claim
22 words
The array of claim 1, wherein each of the different predefined regions is physically separated from each other of the different regions.
Claim 9
Dependent Claim
13 words
The array of claim 1, wherein said planar non-porous solid support is glass.
Claim 10
Dependent Claim
22 words
The array of claim 1, wherein said oligonucleotides are attached to the first surface of the solid support through a linker group.
Claim 11
Dependent Claim
18 words
The array of claim 1, wherein the oligonucleotide in the different predefined regions are at least 20% pure.
Claim 12
Dependent Claim
18 words
The array of claim 1, wherein the oligonucleotides in the different predefined regions are at least 50% pure.
Claim 13
Dependent Claim
18 words
The array of claim 1, wherein the oligonucleotide in the different predefined regions are at least 80% pure.
Claim 14
Dependent Claim
18 words
The array of claim 1, wherein the oligonucleotide in the different predefined regions are at least 90% pure.
Claim 15
Dependent Claim
144 words
The array of claim 1, wherein said array is produced by a binary synthesis process, said process comprising the steps of: providing a planar, non-porous solid support, said solid support having a plurality of compounds immobilized on a surface thereof, said compounds having protecting groups coupled thereto; deprotecting a first portion of said plurality of compounds on said surface and not a second portion of said plurality of compounds; reacting said first portion of said plurality of compounds with a first component of said oligonucleotide; deprotecting at least a third portion of said plurality of compounds on said surface, said third portion comprising a fraction of said first portion of said plurality of compounds; reacting said at least third portion of said plurality of compounds with a second component of said oligonucleotide; and optionally repeating said binary synthesis steps to produce said oligonucleotide array.
Claim 16
Dependent Claim
75 words
An array of polynucleotides, the array comprising: a planar non-porous solid support having at least a first surface; and a plurality of different polynucleotides attached to the first surface of the solid support at a density exceeding 400 different polynucleotides/cm 2 , wherein each of the different polynucleotides is attached to the surface of the solid support in a different predefined region, has a different determinable sequence, and is at least 4 nucleotides in length.
Claim 17
Dependent Claim
75 words
An array of polynucleotides, the array comprising: a planar non-porous solid support having at least a first surface; and a plurality of different polynucleotides attached to the first surface of the solid support at a density exceeding 400 different polynucleotides/cm 2 , wherein each of the different polynucleotides is attached to the surface of the solid support in a different predefined region, has a different determinable sequence, and is at least 4 nucleotides in length.
Claim 18
Dependent Claim
16 words
The array of claim 15, wherein each different polynucleotide is at least 20 nucleotides in length.
Claim 19
Dependent Claim
23 words
The array of claim 15, wherein the array comprises at least 1,000 different polynucleotides attached to the first surface of the solid support.
Claim 20
Dependent Claim
23 words
The array of claim 15, wherein the array comprises at least 10,000 different polynucleotides attached to the first surface of the solid support.
Claim 21
Dependent Claim
22 words
The array of claim 15, wherein each of the different selected regions is physically separated from each of the other selected regions.
Claim 22
Dependent Claim
13 words
The array of claim 15, wherein said planar non-porous solid support is glass.
Claim 23
Dependent Claim
22 words
The array of claim 15, wherein said polynucleotides are attached to the first surface of the solid support through a linker group.
Claim 24
Dependent Claim
21 words
The array of claim 15, wherein the polynucleotides in the different predefined. regions comprise polynucleotides that are at least 20% pure.
Claim 25
Dependent Claim
21 words
The array of claim 15, wherein the polynucleotides in the different predefined regions comprise polynucleotides that are at least 50% pure.
Claim 26
Dependent Claim
18 words
The array of claim 15, wherein the polynucleotides in the different predefined regions are at least 80% pure.
Claim 27
Dependent Claim
17 words
The array of claim 15, the polynucleotides in the different predefined regions are at least 90% pure.
Claim 28
Dependent Claim
138 words
The array of claim 15, wherein said array is produced by a binary synthesis process, said process comprising the steps of: providing a planar, non-porous solid support, said solid support having a plurality of compounds immobilized on a surface thereof, said compounds having protecting groups coupled thereto; deprotecting a first portion of said plurality of compounds on said surface and not a second portion of said plurality of compounds; reacting said first portion of said plurality of compounds with a first reactant; deprotecting at least a third portion of said plurality of compounds on said surface, said third portion comprising a fraction of said first portion of said plurality of compounds; reacting said at least third portion of said plurality of compounds with a second reactant; and optionally repeating said binary synthesis steps to produce said polynucleotide array.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-04-27 | 2001-10-27 | 2002-04-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-04-27 | 2005-10-27 | 2006-04-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-04-27 | 2009-10-27 | 2010-04-28 | $7700.00 | Window Closed / Lapsed |