US5258506A
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Photolabile reagents for incorporation into oligonucleotide chains
Assignee: Bayer Corp
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Filing: 1989-08-25
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Granted: 1993-11-02
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Expires: 2009-08-25
Abstract
Abstract Novel photolabile photochemical reagents are disclosed. The reagents are useful in a variety of biochemical and chemical contexts, including nucleic hybridization assays and chemical phosphorylation of hydroxyl-containing compounds. The reagents are particularly useful for introducing cleavable sites into oligonucleotide or polynucleotide chains, i.e., sites which are cleavable upon photolysis. The reagents are also useful in both 5'- and 3'-phosphorylation of oligonucleotide or polynucleotide chains.
Inventors
- 👤 Michael S. Urdea
- 👤 Thomas Horn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 23:36
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C07; C07H; C07H21/00; C07H21/04
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
46 words
A polynucleotide reagent having the structure ##STR7## wherein DNA 1 is a first segment of DNA; DNA 2 is a second segment of DNA; and one of x and y is zero while the other is an integer in the range of 1 to 12 inclusive.
Claim 2
Dependent Claim
46 words
A polynucleotide reagent having the structure ##STR7## wherein DNA 1 is a first segment of DNA; DNA 2 is a second segment of DNA; and one of x and y is zero while the other is an integer in the range of 1 to 12 inclusive.
Claim 3
Dependent Claim
10 words
The polynucleotide reagent of claim 1, wherein x is zero.
Claim 4
Dependent Claim
19 words
The polynucleotide reagent of claim 2, wherein y is an integer in the range of 1 to 4 inclusive.
Claim 5
Dependent Claim
10 words
The polynucleotide reagent of claim 3, wherein y is one.
Claim 6
Dependent Claim
10 words
The polynucleotide reagent of claim 1, wherein y is zero.
Claim 7
Dependent Claim
19 words
The polynucleotide reagent of claim 5, wherein x is an integer in the range of 1 to 4 inclusive.
Claim 8
Dependent Claim
10 words
The polynucleotide reagent of claim 6, wherein x is one.
Claim 9
Dependent Claim
118 words
A method for detecting the presence of an oligonucleotide sequence of interest in a nucleic acid analyte present in a nucleic acid sample, said method. comprising: combining under hybridizing conditions said nucleic acid sample with the polynucleotide reagent of claim 1, wherein one of said sample or said reagent is bound to a support and hybridization of said analyte and said polynucleotide reagent results in a label being bound to said support through the cleavage site ##STR8## substantially freeing said support of label bound to said support other than through said selectable cleavage site; cleaving said cleavage site via photolysis using light having a wavelength of at least about 350 nm; and detecting label free of said support.
Claim 10
Dependent Claim
150 words
A method for detecting the presence of an oligonucleotide sequence of interest in a nucleic acid analyte present in a nucleic acid sample, said method comprising: combining under hybridizing conditions in an aqueous medium, said nucleic acid sample with the polynucleotide reagent of claim 1, where one of said sample or a component of said reagent is bound to a support and hybridization of said analyte and said polynucleotide reagent results in a label being bound to said support through the cleavage site ##STR9## separating said support having bound polynucleotide reagent and nucleic acid analyte from said aqueous medium; washing said support with a medium of different hybridizing stringency from said aqueous medium to remove label bound to said support other than through said cleavage site; cleaving said cleavage site via photolysis using light having a wavelength of at least about 350 nm; and detecting label free of said support.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1996-11-01 | 1997-05-03 | 1997-11-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-11-01 | 2001-05-03 | 2001-11-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-11-01 | 2005-05-03 | 2005-11-02 | $7700.00 | Window Closed / Lapsed |