US4713326A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Coupling of nucleic acids to solid support by photochemical methods
Assignee: MILES INC. A CORP. OF INDIANA
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Filing: 1984-05-18
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Granted: 1987-12-15
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Expires: 2004-05-18
Abstract
Abstract A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a photochemically reactive intercalator compound or other nucleic acid-binding ligands, and (c) divalent radical chemically linking the substrate and the ligand (b). Specifically, a hydroxy group-containing solid substrate such as nitrocellulose paper is linked via a bifunctional reagent such as cyanogen bromide or 1,4-butanedioldiglycidyl ether to an amino-substituted angelicin or psoralen or ethidium bromide which in turn is photochemically linked to a nucleic acid. The resulting immobilized nucleic acid probe is capable of hybridizing with complementary nucleic acid fragments and is thereby useful in diagnostic assays.
Inventors
- 👤 Nanibhushan Dattagupta
- 👤 Donald M. Crothers
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 21:16
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/548; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
39 words
A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a photochemically reactive nucleic acid-binding ligand, and (c) a divalent radical chemically linking the substrate and the nucleic acid-binding ligand.
Claim 2
Dependent Claim
39 words
A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a photochemically reactive nucleic acid-binding ligand, and (c) a divalent radical chemically linking the substrate and the nucleic acid-binding ligand.
Claim 3
Dependent Claim
27 words
A support according to claim 1, wherein the nucleic acid-binding ligand is an intercalator compound selected from the group consisting of acridine dyes, phenazines, phenothiazines, and quinolines.
Claim 4
Dependent Claim
27 words
A support according to claim 1, wherein the substrate (a) in free state has free OH groups through which it is linked by the divalent radical (c).
Claim 5
Dependent Claim
16 words
A support according to claim 1, wherein the substrate (a) is cellulose or a cellulose ester.
Claim 6
Dependent Claim
16 words
A support according to claim 1, wherein the divalent radical (c) is derived from cyanogen bromide.
Claim 7
Dependent Claim
16 words
A support according to claim 1, wherein the divalent radical (c) is derived from 1,4-butanediol-diglycidyl ether.
Claim 8
Dependent Claim
38 words
An immobilized nucleic acid probe comprising (a) a nucleic acid, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid, and (c) a solid substrate chemically linked through a divalent radical to the nucleic acid-binding ligand (b).
Claim 9
Dependent Claim
38 words
An immobilized nucleic acid probe comprising (a) a nucleic acid, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid, and (c) a solid substrate chemically linked through a divalent radical to the nucleic acid-binding ligand (b).
Claim 10
Dependent Claim
28 words
An immobilized probe according to claim 7, wherein the nucleic acid-binding ligand is an intercalator compound selected from the group consisting of acridine dyes, phenazines, phenothiazines, and quinolines.
Claim 11
Dependent Claim
27 words
An immobilized probe according to claim 7, wherein the substrate (c) in free state has free OH groups through which it is linked by the divalent radical.
Claim 12
Dependent Claim
17 words
An immobilized probe according to claim 7, wherein the substrate (c) is cellulose or a cellulose ester.
Claim 13
Dependent Claim
16 words
An immobilized probe according to claim 7, wherein the divalent radical is derived from cyanogen bromide.
Claim 14
Dependent Claim
16 words
An immobilized probe according to claim 7, wherein the divalent radical is derived from 1,4-butanediol-diglycidyl ether.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-12-14 | 1991-06-15 | 1991-12-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-12-14 | 1995-06-15 | 1995-12-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-12-14 | 1999-06-15 | 1999-12-15 | $7700.00 | Window Closed / Lapsed |