US4542102A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Coupling of nucleic acids to solid support by photochemical methods
Assignee: MILES INC. A CORP. OF INDIANA
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Filing: 1983-07-05
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Granted: 1985-09-17
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Expires: 2003-07-05
Abstract
Abstract A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a member selected from the group consisting of a furocoumarin, a phenanthridium halide, and photochemically reactive derivatives thereof, and (c) a divalent radical chemically linking the substrate and the member (b). Specifically, a hydroxy group-containing solid substrate such as nitrocellulose paper is linked via a bifunctional reagent such as cyanogen bromide or 1,4-butanediol diglycidyl ether to an amino-substituted angelicin or psoralen or phenanthridinium bromide which in turn is photochemically linked to a nucleic acid. This is capable of hybridizing with other nucleic acid fragments and is thereby useful in diagnostic assays.
Inventors
- 👤 Nanibhushan Dattagupta
- 👤 Donald M. Crothers
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:45
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/548; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
58 words
A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a member selected from the group consisting of (1) a furocoumarin, (2) a phenanthridinium halide, and (3) photochemically reactive derivatives of (1) and (2), and (c) a divalent radical chemically linking the solid substrate (a) and the member (b).
Claim 2
Dependent Claim
58 words
A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a member selected from the group consisting of (1) a furocoumarin, (2) a phenanthridinium halide, and (3) photochemically reactive derivatives of (1) and (2), and (c) a divalent radical chemically linking the solid substrate (a) and the member (b).
Claim 3
Dependent Claim
28 words
The support according to claim 1, wherein the solid substrate (a) in free state has free OH groups through which it is linked by the divalent radical (c).
Claim 4
Dependent Claim
17 words
The support according to claim 1, wherein the solid substrate (a) is cellulose or a cellulose ester.
Claim 5
Dependent Claim
16 words
The support according to claim 1, wherein the divalent radical (c) is derived from cyanogen bromide.
Claim 6
Dependent Claim
17 words
The support according to claim 1, wherein the divalent radical (c) is derived from 1,4-butanediol diglycidyl ether.
Claim 7
Dependent Claim
12 words
The support according to claim 1, wherein (b) is an aminomethyl psoralen.
Claim 8
Dependent Claim
12 words
The support according to claim 1, wherein (b) is an aminomethyl angelicin.
Claim 9
Dependent Claim
16 words
The support according to claim 1, wherein (b) is a phenanthridinium (ethidium bromide halide compound) azide.
Claim 10
Dependent Claim
64 words
A hybridization probe comprising (a) a solid substrate, (b) a member selected from the group consisting of (1) a furocoumarin, (2) ethidium bromide, and (3) photochemically reactive derivatives of (1) and (2), (c) a divalent radical chemically linking the solid substrate (a) and the member (b), and (d) a nucleic acid photochemically linked to member (b) and fixed thereby to the solid substrate (a).
Claim 11
Dependent Claim
64 words
A hybridization probe comprising (a) a solid substrate, (b) a member selected from the group consisting of (1) a furocoumarin, (2) ethidium bromide, and (3) photochemically reactive derivatives of (1) and (2), (c) a divalent radical chemically linking the solid substrate (a) and the member (b), and (d) a nucleic acid photochemically linked to member (b) and fixed thereby to the solid substrate (a).
Claim 12
Dependent Claim
29 words
The hybridization probe according to claim 9, wherein the solid substrate (a) in free state has free OH groups through which it is linked by the divalent radical (c).
Claim 13
Dependent Claim
19 words
The hybridization probe according to the claim 9, wherein the solid substrate (a) is cellulose or a cellulose ester.
Claim 14
Dependent Claim
20 words
The hybridization probe according to claim 9, wherein the divalent radical (c) is obtained by the activation by cyanogen bromide.
Claim 15
Dependent Claim
18 words
The hybridization probe according to claim 9, wherein the divalent radical (c) is derived from 1,4-butanediol diglycidyl ether.
Claim 16
Dependent Claim
16 words
The hybridization probe according to claim 9, wherein member (b) is psoralen or a derivative thereof.
Claim 17
Dependent Claim
16 words
The hybridization probe according to claim 9, wherein member (b) is angelicin or a derivative thereof.
Claim 18
Dependent Claim
17 words
The hybridization probe according to claim 9, wherein member (b) is phenanthridinium halide or a derivative thereof.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-09-16 | 1989-03-18 | 1989-09-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-09-16 | 1993-03-18 | 1993-09-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-09-16 | 1997-03-18 | 1997-09-17 | $7700.00 | Window Closed / Lapsed |