US5734587A
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US
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Method of analyzing clinically relevant liquids and suspensions
Assignee: Roche Diagnostics GmbH
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Filing: 1994-09-19
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Granted: 1998-03-31
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Expires: 2014-09-19
Abstract
Abstract A method of analyzing clinically relevant sample liquids and suspensions is such that where infrared spectra of dried samples are generated and evaluated using a multivariate evaluation procedure. In the evaluation procedure, the samples to be analyzed are assigned to classes. The evaluation procedure is trained with samples of known classes to adjust the parameters of the evaluation procedures such that samples of unknown classification can be assigned to known classes. In an advantageous manner, the infrared spectra can be generated by a transmittance measurement on a dried film of the sample.
Inventors
- 👤 Juergen Backhaus
- 👤 Dirk Boecker
- 👤 Reinhard Mischler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 15:56
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/508; B01L3/5085; G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/31; G01N21/35; G01N21/3577; G01N21/47; G01N1/00; G01N1/28; G01N1/40; G01N1/4022; G01N2001/4027; G01N2201/00; G01N2201/12; G01N2201/129; Y; Y10; Y10T; Y10T436/00; Y10T436/11; Y10T436/112499; Y10T436/115831
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
123 words
A method of analyzing clinically relevant liquids and/or suspensions, comprising a) measuring the infrared spectra of a plurality of samples belonging to known classes, b) implementing a multivariate evaluation procedure and training the multivariate evaluation procedure by selecting parameters ensuring a reliable assignment of the samples to the known classes, c) storing the parameters of the multivariate evaluation procedures obtained, d) providing a sample of the liquid and/or suspension to be analyzed, e) applying the sample to a carrier, f) drying the sample, g) exposing the sample to infrared radiation, h) generating an infrared spectrum of the sample, i) analyzing the infrared spectrum by means of a multivariate evaluation procedure, and j) assigning the sample to a class of the multivariate evaluation procedure.
Claim 2
Independent Claim
123 words
A method of analyzing clinically relevant liquids and/or suspensions, comprising a) measuring the infrared spectra of a plurality of samples belonging to known classes, b) implementing a multivariate evaluation procedure and training the multivariate evaluation procedure by selecting parameters ensuring a reliable assignment of the samples to the known classes, c) storing the parameters of the multivariate evaluation procedures obtained, d) providing a sample of the liquid and/or suspension to be analyzed, e) applying the sample to a carrier, f) drying the sample, g) exposing the sample to infrared radiation, h) generating an infrared spectrum of the sample, i) analyzing the infrared spectrum by means of a multivariate evaluation procedure, and j) assigning the sample to a class of the multivariate evaluation procedure.
Claim 3
Dependent Claim
18 words
Method of claim 1, wherein the carrier reflects infrared radiation to generate a reflection spectrum of the sample.
Claim 4
Dependent Claim
13 words
Method of claim 2 wherein the reflection spectrum is a diffuse reflection spectrum.
Claim 5
Dependent Claim
23 words
Method of claim 1, wherein the liquids and/or suspensions which are analyzed are body fluids which are whole blood, hemolysate, serum and/or plasma.
Claim 6
Dependent Claim
13 words
Method of claim 1, wherein a suspension of cell and/or bacteria is analyzed.
Claim 7
Dependent Claim
17 words
Method of claim 2, wherein in step (g), the entire sample is irradiated with the infrared radiation.
Claim 8
Dependent Claim
32 words
Method of claim 1, wherein steps (a), (b), and (c) are carried out one or more times while steps (d) to (j) are carried out in multiples and cyclically using different samples.
Claim 9
Dependent Claim
20 words
Method of claim 1, wherein the multivariate evaluation procedure is a discriminant analysis, a neuronal net, or a cluster analysis.
Claim 10
Dependent Claim
13 words
Method of claim 8, wherein the discriminant analysis is a regularized discriminant analysis.
Claim 11
Dependent Claim
16 words
Method of claim 1, wherein infrared spectrum ranging from 400 to 15000 cm -1 are generated.
Claim 12
Dependent Claim
16 words
Method of claim 10, wherein infrared spectrum ranging from 400 to 4000 cm -1 are generated.
Claim 13
Dependent Claim
23 words
Method of claim 1, wherein the samples of known classification are samples from which it is known that an internal disease is present.
Claim 14
Dependent Claim
25 words
Method of claim 1, wherein the samples of known classification are samples from which it is known that a liver or renal disease is present.
Claim 15
Dependent Claim
21 words
Method of claim 1, wherein the samples of known classification are samples of patients with type I or type II diabetes.
Claim 16
Dependent Claim
20 words
Method of claim 1, wherein additionally at least one sample is used which is a sample of a healthy patient.
Claim 17
Dependent Claim
90 words
Method of analyzing clinically relevant liquids and/or suspensions, comprising a) providing a sample of the liquid and/or suspension, b) placing the sample onto a carrier having at least one opening in which the sample forms a hanging film, c) drying the sample, d) allowing radiation to traverse the dried sample contained in the opening, e) detecting radiation transmitted across the dried sample to generate an infrared spectrum, f) evaluating the infrared spectrum to determine the concentration of at least one analyte contained in the sample, and/or to classify the sample.
Claim 18
Dependent Claim
90 words
Method of analyzing clinically relevant liquids and/or suspensions, comprising a) providing a sample of the liquid and/or suspension, b) placing the sample onto a carrier having at least one opening in which the sample forms a hanging film, c) drying the sample, d) allowing radiation to traverse the dried sample contained in the opening, e) detecting radiation transmitted across the dried sample to generate an infrared spectrum, f) evaluating the infrared spectrum to determine the concentration of at least one analyte contained in the sample, and/or to classify the sample.
Claim 19
Dependent Claim
18 words
Method of claim 16, wherein the at least one opening has a diameter of 200 to 1000 μm.
Claim 20
Dependent Claim
16 words
Method of claim 16, wherein the carrier is an eye, a net or a perforated foil.
Claim 21
Dependent Claim
38 words
Method of claim 16, wherein a defined quantity of a reference substance is added to the sample, and the amount of the sample material detected by the infrared beam is determined from the absorption of the reference substance.
Claim 22
Dependent Claim
33 words
Method of claim 16, wherein the thickness of the suspended film is interferometrically determined, and the thickness of the film is used to determine the amount of sample detected by the infrared beam.
Claim 23
Dependent Claim
21 words
Method of claim 16, wherein film-forming substances are added to the sample liquid to increase the stability of the hanging film.
Claim 24
Dependent Claim
21 words
Method of claim 16, wherein the infrared spectrum obtained in step (e) is analyzed by means of a multivariate evaluation procedure.
Claim 25
Dependent Claim
20 words
Method of claim 22, wherein the multivariate evaluation procedure is a discriminant analysis, a neuronal net, or a cluster analysis.
Claim 26
Dependent Claim
13 words
Method of claim 23, wherein the discriminant analysis is a regularized discriminant analysis.
Claim 27
Dependent Claim
16 words
Method of claim 16, wherein infrared spectrum ranging from 400 to 15000 cm -1 are generated.
Claim 28
Dependent Claim
16 words
Method of claim 25, wherein infrared spectrum ranging from 400 to 4000 cm -1 are generated.
Claim 29
Dependent Claim
12 words
Method of claim 16, wherein cell/bacteria suspensions are used as the samples.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-03-30 | 2001-09-29 | 2002-03-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-03-30 | 2005-09-29 | 2006-03-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-03-30 | 2009-09-29 | 2010-03-31 | $7700.00 | Window Closed / Lapsed |