EP0419222A2
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EP
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Method for the prediction of properties of biological matter by analysis of the near-infrared spectrum thereof
Assignee: University of Washington
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Filing: 1990-09-18
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Granted: —
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Expires: 2010-09-18
Abstract
Abstract A method is provided for predicting a property of a matter of biological origin, such as biological fluid, containing water, where the biological matter may be approximated to contain two compartments where one compartment has a proportionally larger or smaller amount of water than the other compartment having the property of interest. The method involves establishing a training set in the near-infrared (NIR) region with independent quantification of the property of the matter using known techniques. The training set is mathematically analyzed according to a correlation developed by regression analysis after employment of a ratio pre-processing technique. The result is a mathematical transformation equation which quantitatively relates spectral intensities at specific wavelengths to the property of interest. This transformation equation may be applied to unknown samples so as to predict their properties, thereby eliminating need for the reference method except for validation or recalibration. The method provides rapid and accurate prediction of the property of the unknown sample, which may be the property of hematocrit or hemoglobin concentration in whole animal blood.
Inventors
- 👤 David W. C/O Minnesota Mining And Osten
- 👤 James B. C/O Board Of Regents Callis
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 18:48
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/31; G01N21/35; G01N21/359; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/49; G01N33/492
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
191 words
A method for analyzing a property of biological matter having a water content, the biological matter comprising a first compartment related to the property to be analyzed and a second compartment having a proportionally larger or smaller amount of water than the first compartment, the method comprising: (a) obtaining multiple samples of biological matter from at least one known organism of a given species; (b) irradiating with near infrared light said multiple samples; (c) detecting the near infrared spectrum of each of said multiple samples; (d) applying a ratio pre-processing technique to the spectrum of each of said multiple samples; (e) independently quantifying the property to be analyzed for each of said multiple samples; (f) establishing a training set from said near infrared spectra of said multiple samples; (g) statistically identifying the nature of a mathematical correlation between the property to be analyzed in the first compartment and the water content in the biological matter; wherein said ratio pre-processing technique comprises applying a ratio of a near infrared wavelength absorbance peak of the water content in said training set to another near infrared wavelength absorbance measuring point in said training set.
Claim 2
Independent Claim
191 words
A method for analyzing a property of biological matter having a water content, the biological matter comprising a first compartment related to the property to be analyzed and a second compartment having a proportionally larger or smaller amount of water than the first compartment, the method comprising: (a) obtaining multiple samples of biological matter from at least one known organism of a given species; (b) irradiating with near infrared light said multiple samples; (c) detecting the near infrared spectrum of each of said multiple samples; (d) applying a ratio pre-processing technique to the spectrum of each of said multiple samples; (e) independently quantifying the property to be analyzed for each of said multiple samples; (f) establishing a training set from said near infrared spectra of said multiple samples; (g) statistically identifying the nature of a mathematical correlation between the property to be analyzed in the first compartment and the water content in the biological matter; wherein said ratio pre-processing technique comprises applying a ratio of a near infrared wavelength absorbance peak of the water content in said training set to another near infrared wavelength absorbance measuring point in said training set.
Claim 3
Dependent Claim
82 words
A method according to Claim 1, further comprising the steps of: (h) obtaining an unknown sample of biological matter from an organism of said given species; (i) irradiating said unknown sample with near infrared light; (j) detecting the near infrared spectrum of said unknown sample; (k) applying said ratio pre-processing technique to said spectrum of said unknown sample; and (l) predicting the property to be analyzed in said unknown sample by utilizing said mathematical correlation obtained in said statistically identifying step (g).
Claim 4
Dependent Claim
29 words
A method according to Claim 1, wherein said statistically identifying step (g) uses linear regression analysis, multiple linear regression analysis, stepwise regression analysis, or partial least squares regression analysis.
Claim 5
Dependent Claim
36 words
A method according to Claim 1 or Claim 2, wherein the biological matter is whole blood and the property of the first compartment to be analyzed is hematocrit or hemoglobin in whole blood of the organism.
Claim 6
Dependent Claim
51 words
A method according to Claim 1 or Claim 2, wherein the biological matter is whole blood and said absorbance peak of water occurs in the near infrared spectra from about 1150 to about 1190 nanometers and said another absorbance measuring point is the isosbestic point of absorbance of oxyhemoglobin and deoxyhemoglobin.
Claim 7
Dependent Claim
37 words
A method according to Claim 2, wherein said detecting step (c) and said detecting step (j) use spectral analysis instrumentation which records said absorbance spectra of said multiple samples and said unknown sample in a static condition.
Claim 8
Dependent Claim
78 words
A method according to Claim 2, wherein the property to analyzed is hematocrit and said mathematical correlation solves the equation: Y = b + m * (Absorbance at an Isosbestic Point of Deoxyhemoglobin and Oxyhemoglobin/Absorbance of said Absorbance Peak of Water) where Y is the value of hematocrit, b ranges from about -150 to about -183, m ranges from about 153 to about 181, and said Absorbance Peak of Water ranges from about 1150 to about 1170 nm.
Claim 9
Dependent Claim
78 words
A method according to Claim 2, wherein the property to be analyzed is hemoglobin concentration and said mathematical correlation solves the equation: Y = b + m * (Absorbance at an Isosbestic Point of Deoxyhemoglobin and Oxyhemoglobin/Absorbance of said Absorbance Peak of Water) where Y is the concentration of hemoglobin, b ranges from about-50 to about -65, m ranges from about 51 to about 64, and said Absorbance Peak of Water ranges from about 1150 to 1170 nm.
Claim 10
Dependent Claim
83 words
A method according to Claim 1, further comprising the steps of: (1) obtaining additional samples of biological matter from an organism of said given species; (2) performing steps (b), (c), (d), (e) with respect to said additional samples; (3) predicting the property to be analyzed in said additional samples by utilizing said mathematical correlation obtained in said statistically identifying step (g); and (4) validating said mathematical correlation by comparing the property predicted in step (3) to the property independently quantified in step (e).
Claim 11
Independent Claim
152 words
A method for the analysis of a biological fluid of an organism, comprising: obtaining multiple samples of a biological fluid from at least one least one organism, where the biological fluid may be approximated to comprise two compartments where one compartment has a proportionally different amount of water than the other compartment which has a property of interest; determining the two best absorbance measuring points of the near infrared spectrum of each of said multiple samples; independently measuring the property of interest in each of said multiple samples; applying a ratio of said two best absorbance measuring points; identifying a mathematical correlation of the property to be analyzed and water using the ratio of said two best absorbance measuring points; and analyzing an unknown sample of the biological fluid to predict the property of interest in said unknown sample by applying said mathematical correlation to near infrared spectrum of said unknown sample.
Claim 12
Independent Claim
152 words
A method for the analysis of a biological fluid of an organism, comprising: obtaining multiple samples of a biological fluid from at least one least one organism, where the biological fluid may be approximated to comprise two compartments where one compartment has a proportionally different amount of water than the other compartment which has a property of interest; determining the two best absorbance measuring points of the near infrared spectrum of each of said multiple samples; independently measuring the property of interest in each of said multiple samples; applying a ratio of said two best absorbance measuring points; identifying a mathematical correlation of the property to be analyzed and water using the ratio of said two best absorbance measuring points; and analyzing an unknown sample of the biological fluid to predict the property of interest in said unknown sample by applying said mathematical correlation to near infrared spectrum of said unknown sample.
References Cited (Backward Citations)
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