Patent US2357686A successfully refreshed from Google Patents.
Patent US5618718A successfully refreshed from Google Patents.
US5618718A
Expired / Lapsed
US
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Production of a contractile smooth muscle
Assignee: Université Laval
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Filing: 1994-12-30
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Granted: 1997-04-08
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Expires: 2014-12-30
Abstract
Abstract A contractile smooth muscle cell construct and a method for preparing the smooth muscle cell construct are described. The smooth muscle cell construct is comprised of (i) smooth muscle cells that have been cultured in vitro under conditions to allow the formation of a sheet of smooth muscle cells and (ii) an endogenous fibrous matrix formed by the smooth muscle cells, wherein the smooth muscle cell construct retains the ability to contract in response to vasoactive agonists. The smooth muscle cell construct may be prepared in planar or tubular form and can be used to study or evaluate the contractile responses of smooth muscle cells.
Inventors
- 👤 François A. Auger
- 👤 Nicolas L'Heureux
- 👤 Lucie Germain
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 19:58
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/069; C12N5/0691; C12N2503/00
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
60 words
A contractile smooth muscle cell construct comprising: i) sub-cultured human smooth muscle cells that have been cultured in vitro under conditions to allow the formation of a sheet of smooth muscle cells; and ii) an endogenous fibrous matrix formed by the smooth muscle cells; wherein the smooth muscle cell construct retains the ability to contract in response to vasoactive agonists.
Claim 2
Dependent Claim
60 words
A contractile smooth muscle cell construct comprising: i) sub-cultured human smooth muscle cells that have been cultured in vitro under conditions to allow the formation of a sheet of smooth muscle cells; and ii) an endogenous fibrous matrix formed by the smooth muscle cells; wherein the smooth muscle cell construct retains the ability to contract in response to vasoactive agonists.
Claim 3
Dependent Claim
20 words
A contractile smooth muscle cell construct according to claim 1 wherein said smooth muscle cell construct is in tubular form.
Claim 4
Dependent Claim
84 words
A method for producing the contractile smooth muscle cell construct of claim 1 which comprises: a) culturing sub-cultured human smooth muscle cells in a culture vessel with a culture medium that promotes the synthesis of a fibrous matrix by the cells; b) maintaining the cells in culture until the cells adhere to the culture vessel, become confluent and the cells and the matrix form a sheet; and c) allowing the cell sheet to spontaneously detach or detaching the cell sheet from the culture vessel.
Claim 5
Dependent Claim
13 words
The method according to claim 3, wherein said culture medium contains ascorbic acid.
Claim 6
Dependent Claim
23 words
The method according to claim 3 further comprising rolling said cell sheet around a tubular support to produce a tubular living tissue construct.
Claim 7
Dependent Claim
26 words
The method according to claim 5, further comprising: removing the tubular living tissue construct from the support and maintaining said tubular living tissue construct in culture.
Claim 8
Dependent Claim
21 words
The method according to claim 5 further comprising seeding endothelial cells on the inner surface of said tubular living tissue construct.
Claim 9
Dependent Claim
36 words
A method of producing a multilayered tubular living tissue construct said method comprising: a) creating a tubular living tissue construct according to claim 4; b) rolling a second cell sheet around said tubular living tissue construct.
Claim 10
Dependent Claim
12 words
The method according to claim 8 wherein said second sheet comprises fibroblasts.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-04-07 | 2000-10-07 | 2001-04-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-04-07 | 2004-10-07 | 2005-04-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-04-07 | 2008-10-07 | 2009-04-08 | $7700.00 | Window Closed / Lapsed |