US5230693A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Implantable prosthetic device for implantation into a human patient having a surface treated with microvascular endothelial cells
Assignee: Thomas Jefferson University
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Filing: 1991-06-27
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Granted: 1993-07-27
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Expires: 2011-06-27
Abstract
Abstract The present invention provides a synthetic or naturally occurring implant, such as a vascular graft, for implantation into a human patient. Uncultured, microvascular endothelial cells, isolated from microvascular endothelial cell rich tissue are disposed on at least one surface of the implant to provide at least about 50% confluence of the cells on the surface of the implant prior to implantation. In a preferred embodiment, the microvascular endothelial cells are obtained from fat tissue. Because of the large number of fresh microvascular endothelial cells that may be obtained from such tissue, sufficient cells may be placed on the implant so that they attach to provide at least 50% confluent coverage of the implant surface prior to the time of implantation.
Inventors
- 👤 Stuart K. Williams
- 👤 Bruce E. Jarrell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 06:19
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/50; A61L27/507; A61L27/28; A61L27/34; A61L27/36; A61L27/38
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
51 words
An implant for implantation into a human patient, having disposed on at least one surface thereof uncultured human microvascular endothelial cells obtained from human microvascular endothelial cell rich tissue, wherein said uncultured cells are at least about 50% confluent on the surface of said implant prior to the time of implantation.
Claim 2
Dependent Claim
51 words
An implant for implantation into a human patient, having disposed on at least one surface thereof uncultured human microvascular endothelial cells obtained from human microvascular endothelial cell rich tissue, wherein said uncultured cells are at least about 50% confluent on the surface of said implant prior to the time of implantation.
Claim 3
Dependent Claim
17 words
The implant of claim 1 wherein said uncultured cells are adhered to said surface prior to implantation.
Claim 4
Dependent Claim
12 words
The implant of claim 1 wherein said implant is a vascular graft.
Claim 5
Dependent Claim
13 words
The implant of claim 3 wherein said vascular graft is a polyester graft.
Claim 6
Dependent Claim
18 words
The implant of claim 4 wherein said polyester is a condensation product of methyl terephthalate and ethylene glycol.
Claim 7
Dependent Claim
15 words
The implant of claim 4 wherein said polyester has been surface modified to emulate protein.
Claim 8
Dependent Claim
23 words
The implant of claim 1 wherein said implant comprises a polyester surface coated with plasma protein and with uncultured human microvascular endothelial cells.
Claim 9
Dependent Claim
18 words
The implant of claim 1 wherein said uncultured human microvascular endothelial cells are preclotted in a protein gel.
Claim 10
Dependent Claim
13 words
The implant of claim 8 where said protein gel is a fibrin gel.
Claim 11
Dependent Claim
17 words
The implant of claim 1 wherein said uncultured human microvascular endothelial cells are preclotted in human plasma.
Claim 12
Dependent Claim
16 words
The implant of claim 1 wherein said human microvascular endothelial cell rich tissue is fat tissue.
Claim 13
Dependent Claim
28 words
The implant of claim 11 wherein said tissue is selected from the group consisting of fat associated with the thoracic or peritoneal cavities, perinephric fat and subcutaneous fat.
Claim 14
Dependent Claim
10 words
The implant of claim 1 wherein said tissue is omentum.
Claim 15
Dependent Claim
59 words
An implant for implantation into a human patient, having disposed on at least one surface thereof uncultured human microvascular cells obtained from human microvascular endothelial cell rich tissue from that patient, wherein said uncultured cells are applied onto said implant to provide at least about 50% confluence on the surface of said implant prior to the time of implantation.
Claim 16
Dependent Claim
59 words
An implant for implantation into a human patient, having disposed on at least one surface thereof uncultured human microvascular cells obtained from human microvascular endothelial cell rich tissue from that patient, wherein said uncultured cells are applied onto said implant to provide at least about 50% confluence on the surface of said implant prior to the time of implantation.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-07-26 | 1997-01-25 | 1997-07-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-07-26 | 2001-01-25 | 2001-07-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-07-26 | 2005-01-25 | 2005-07-27 | $7700.00 | Window Closed / Lapsed |