US5254471A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Carrier for cell culture
Assignee: Toray Industries Inc
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Filing: 1992-01-13
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Granted: 1993-10-19
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Expires: 2012-01-13
Abstract
Abstract A carrier for cell culture comprising ultra fine fibers with a fineness of not more than 1.0 denier. The carrier makes it possible for the cells to retain their differentiation and proliferation ability for a long time. It also enables a large scale culturing.
Inventors
- 👤 Yuuichi Mori
- 👤 Shigeyasu Kobayashi
- 👤 Miyoshi Okamoto
- 👤 Kouji Watanabe
- 👤 Shouji Nagaoka
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 13:40
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N2533/00; C12N2533/30
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
45 words
In a method for culturing animal and plant cells in which the cells differentiate and proliferate on a carrier, the improvement which comprises producing, as a carrier for the cell culture, a composite of ultra fine fibers having a fineness of less than 0.5 denier.
Claim 2
Dependent Claim
45 words
In a method for culturing animal and plant cells in which the cells differentiate and proliferate on a carrier, the improvement which comprises producing, as a carrier for the cell culture, a composite of ultra fine fibers having a fineness of less than 0.5 denier.
Claim 3
Dependent Claim
28 words
The method of claim 1, wherein the ultra fine fibers are formed into configurations selected from the group consisting of films, rods, spheres, flat rectangles, bellows, and spirals.
Claim 4
Dependent Claim
24 words
The method of claim 1, wherein the ultra fine fibers are in the form of a texture which is impregnated with a collagen gel.
Claim 5
Dependent Claim
43 words
The method of claim 1, wherein the cells to be cultured are endothelial or epithelial cells selected from the group consisting of vascular endothelial cells, smooth muscle cells, hepatic parenchyma cells, pancreas β cells, epithelium cells, intestine cells, fibroblasts and other parenchyma cells.
Claim 6
Dependent Claim
31 words
The method of claim 1, wherein the ultra fine fibers are made of one or more polymers selected from the group consisting of polyesters, polyamides, polyolefins, cellulose, polyamino acids, and collagen.
Claim 7
Dependent Claim
18 words
The method of claim 5, wherein one of said polymers contains a diethylaminoethyl group with a positive charge.
Claim 8
Dependent Claim
40 words
The method of claim 5, wherein additional fiber components are combined therewith, said additional fibers being made from polymers selected from the group consisting of polystyrenes, polyethylenes, water soluble polyamides, polyesters soluble in aqueous alkaline solutions, and water-soluble polyvinyl alcohols.
Claim 9
Dependent Claim
23 words
The method of claim 1, wherein said fibers are in the form of a textured woven or non-woven fabric, knit, or spun yarn.
Claim 10
Dependent Claim
16 words
The method of claim 8, wherein the interval between the fibers is at least ten micrometers.
Claim 11
Dependent Claim
18 words
The method of claim 8, wherein the ultra fine fibers are napped to increase the surface contact area.
Claim 12
Dependent Claim
17 words
The method of claim 1, wherein said fibers are made by removing a component from multi-component fibers.
Claim 13
Dependent Claim
10 words
The method of claim 11, wherein said fibers are polyester.
References Cited (Backward Citations)
US3418200A
Cited
US3516239A
Cited
US4087327A
Cited
US4368227A
Cited
US4189534A
Cited
US4610962A
Cited
US4957868A
Cited
GB2178447A
Cited
US5043278A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-10-18 | 1997-04-19 | 1997-10-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-10-18 | 2001-04-19 | 2001-10-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-10-18 | 2005-04-19 | 2005-10-19 | $7700.00 | Window Closed / Lapsed |